Objective To investigate the influence of systematic nursing intervention on the quality of life of patients with radical cystectomy and bricker operation.Methods 67 patients with radical cystectomy and bricker operation were randomly divided into control group ( n = 33) and observation group ( n = 34).Control group was routinely cared, while the observation group was cared with systematic nursing intervention by the nurse in charge of work.Then all the patients were surveyed with the core questionnaire of quality of life of cancer patients (QLQ-C30) in 6 months after the operation.Results The scores of global health status, physical function, role function,emotional function,cognitive function and social function of the observation group (64.8 ± 8.7,80.1 ±9.2,67.5 ± 10.1,82.1 ± 11.5,84.6 ± 14.2,66.4 ± 12.5 ) were higher than those of the control group ( 53.3 ±9.4,68.3 ± 8.8,56.5 ± 10.3,68.3 ± 12.2,67.1 ± 10.5,55.3 ± 12.3 ), respectively.The scores of fatigue, nausea and vomiting, insomnia, and appetite loss of the observation group (34.6 ± 8.9,19.4 ± 6.9,27.7 ± 7.6,25.7± 8.4) were lower than those of the control group (44.4 ± 10.3,30.5 ± 7.6,39.8 ± 7.8,34.8 ± 10.1 ), respectively.And the difference was significant (P ＜ 0.05 ).Conclusion The systematic intervention can enhance patients' psychological, physical, and social adaptability, and improve patients' self-care skills and quality of life.

OBJECTIVETo develop a method for detecting the topical concentration of hydrocortisone (HC) in the subcutaneous adipose of rats using microdialysis sampling technique and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS).

METHODSTopical samples were collected by applying the probe into the subcutaneous adipose of rats, with alcohol (5%)-ringers solution as the perfusion solution. A LC-MS/MS method was established for detecting the HC concentration in the dialysates.

RESULTSThe protonated precursor to produce ion transitions monitored for HC was m/z 363.2→121.1. The calibration curve was linear over the range of 0.5-1000 ng/ml, with the intra- and inter-day precision and accuracy within ∓15%, and no significant matrix effect was noted. The in vivo recovery of the probe was about 59%.

CONCLUSIONA selective and sensitive method has been successfully established for the on-line HC detection in the subcutaneous adipose of rats.

Administration, Cutaneous ; Animals ; Chromatography, Liquid ; methods ; Hydrocortisone ; administration & dosage ; pharmacokinetics ; Male ; Microdialysis ; methods ; Rats ; Rats, Wistar ; Subcutaneous Fat ; chemistry ; metabolism ; Tandem Mass Spectrometry ; methods