ObjectTo study the application of ultrasonic circulation technique for the extraction of triterpenoids from Ganoderma lucidum (Leyss.ex Fr.) Karst. Methods On the basis of conventional method, the processing steps of ultrasonic circulation treatment were added. Results The comparative experiments showed that less amounts of various solvents and shorter extraction time were needed for ultrasonic circulation extraction, with the product ratio of about 40 percent higher than that of conventional method. Furthermore, a good consistency of the target product analyzed by HPLC was found between two different extraction methods. Conclusion The ultrasonic circulation technique has a potential application to the extraction of triterpenoids from G. lucidum.

Eguus asinus is one of the rare Chinese drugs famous for promoting blood circulation. In this experiment, it was employed to modify the silk fibroin (SF) by physical blending. Mouse embryonic fibroblasts NIH-3T3 were seeded on pure and modified SF surfaces. The morphological changes of cell on SF surfaces were characterized by optical microscope images. The cell adhesion diameter, the attachment force (F) of a single cell and cell proliferation on SF surfaces were measured by micropipette aspiration system and MTT assay. The results indicated that the fibroblasts seeded on modified SF had larger cell adhesion area, stronger initial attachment force and higher cell proliferation than did the pure SF. In conclusion, eguus asinus improved the adhesion and proliferation of fibroblasts on SF. The research promoted the further use of Chinese drug eguus asinus in tissue engineering.
Animals ; Cell Adhesion ; drug effects ; Cell Proliferation ; drug effects ; Drugs, Chinese Herbal ; chemistry ; Fibroblasts ; cytology ; Fibroins ; chemistry ; Mice ; NIH 3T3 Cells ; Surface Properties

The micropipette aspiration technique was improved by selecting the uniform cells in round shape (UCR) from a number of adhered cells on the testing surface so as to measure the adhesion forces during the initial adhesion stage. Silk fibroin film and silk fibroin/fucoidan film were used as testing substrates. The testing results revealed that the modified micropipette method was more rapid for testing. The tendency of cell adhesion force on different materials surface under different conditions remained unchanged. The testing results showed good reproducibility. It was easy to distinguish small differences in the adhesiveness of endothelial cell on the silk fibroin based materials, thus exhibiting the high sensitivity of the improved method.
Cell Adhesion ; physiology ; Cells, Cultured ; Endothelial Cells ; cytology ; Fibroins ; Humans ; Umbilical Veins ; cytology