1.Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction
Yimei DU ; Ming TANG ; Changjin LIU ; Zhigang HONG ; Qinmei KE ; Jiufang DI ; Hongyan LUO ; Mouxian HU ; Xinwu HU ; Jiaoya XI ; Bi TANG ; Heschele JURGEN
Chinese Journal of Pathophysiology 1999;0(09):-
AIM:To determine the role of Kv1 2, Kv1 5, Kv2 1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1 2/Kv1 5/Kv2 1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells [from (-51 8?0 8) mV to (-47 2? 0 7) mV, P
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