Objective To assess the performance capability of syphilis serology among STI laborato-ries in Guangdong Province. Methods Positive and negative sera samples were prepared and delivered toSTI laboratories. Non-treponemal and treponemal quantitative and qualitative tests were performed with these samples based on standardized procedures. Quality and proficiency of syphilis serology among these labora-tories were evaluated. Results A total of 225 STI laboratories in Guangdong Province participated in this survey, 3696 serological tests for syphilis were performed from 2004-2006. The outcomes showed that the total reproducibility among participating laboratories increased from 82.5% in 2004 to 90.0% in 2006 (x2 =16.8, P ＜ 0.05), and the percentage of laboratories with a performance level higher than the provincial average level rose from 64.7% in 2004 to 76.8% in 2006. The total reproducibility was 95.5% for non-treponemal qualitative tests,95.0% for treponemal qualitative tests, 82.1% for non-treponemal quantitative tests, and 81.1% for treponemal quantitative tests. The false-negativity and false-positivity rates were 4.7% and 4.0%,respectively, for non-treponemal qualitative tests, and 7.2% and 0.4%, respectively, for treponemal qualitative tests. Conclusions It is important to do capability building and improve the performance of STI laborato-ries, in order to prevent and control syphilis.

0 05).CONCLUSION:Na +-K +-ATPase is not sensitive to ischemic damage,but sensitive to reperfusion damage.Like hypothermia,scopolamine can protect Na +-K +-ATPase activity in the cerebral cortex during postischemic reperfusion,but hypothermia has no cooperating effect on scopolamine.

0.05).The total treatment costs were(1084.43?247.38)yuan for the treatment group and(1211.23?275.95)yuan for the controlled group,which had significant difference(P0.05).The total costs were(1270.45?218.12)yuan for the treatment group and(1407.51?51.01)yuan for the controlled group(P

In order to reinforce the development and sharing of elaborate educational resources and improve the educational quality, the course team completed the transformation and upgrading of the original fine course for the construction of national high-quality resource sharing class. In the con-struction of courses the organic combination of course content, method, skills, quality was focused on, and the transformation and upgrading of the course achieved further optimization of the excellent resources and full sharing.

Objective To detect Pneumocystis carinii (Pc) DNA by loop-mediated isothermal amplification (LAMP). Methods After injected with hydrocortisone acetate for 8 weeks, the bronchoalveolar lavage fluid (BALF) of Wistar rats were collected and a portion of BALF were examined for identifying Pc organisms using microscope. Then Pc DNA was extracted by phenol-chloroform extraction. Four primers which recognized 6 distinct regions on the mtrRNA gene of Pc were designed and used for LAMP assay. To evaluate the specificity of the assay, M. tuberculosis, M. pneumoniae, C. pneumoniae, P. gondii and rat leucocyte were used as negative controls. To compare the sensitivity of the LAMP to that of conventional PCR, Pc DNA were 10-fold serially diluted and was amplified by LAMP and PCR. LAMP results were judged by naked eye, electrophoretic analysis and restriction digestion. Results Pc organisms were detected from BALF of rats injected with hydrocortisone acetate. After LAMP reaction, positive signal was observef rats injected with hydrocortisone acetate. By contrast, no positive signal was observed for the negative controls in the study. The amplified product digested by restriction enzyme demonstrated 3 bands (82, 135, 189 lip) upon agarose gel electrophoresis, in good agreement with the predicted sizes. The detection limit of LAMP assay was 1 pg/μl of Pc DNA per reaction and that of PCR was 10 pg/μ1 of Pc DNA per reaction. Conclusion LAMP assay has usefulness for rapid detection of Pc.

Objective To discuss clinical application of descending genicular artery perforator flap.Methods According to the anatomic features of direction,branches and anastomosis of the descending genicular artery,the descending genicular genicular artery perforator flap at medial superior aspect of knee joint was designed to reconstruct the soft tissue defects at the anterior medial 1/3 of the calf and the anterior medial part and popliteal fossa of the knee,with the axis based on the anterior border of the Sartorius and with the pivot point on the site where the cutaneous branches from the superior medial genicular artery pierced out within the triangle concave surface bounded by the vastus medialis,the tendon of adductor magnus and the condylus medialis.Results All flaps survived well in five patients,with primary healing.After a follow-up of 1-12 months,all flaps turned out to be with good texture,near-normal color and good appearance.Conclusion With a constant anatomic location,excellent blood supply and easy surgical procedure,the descending genicular artery perforator flap is one of feasible ways for repair of soft tissue defects around the knee.

BACKGROUND:Intervertebral disc can bear load but lack vessels. Nucleus pulposus cel s have the problem of phenotype loss during in vitro culture that can lead to degenerative changes. The mechanism of intervertebral disc degeneration remains unclear.
OBJECTIVE:To explore the approaches of isolation, adherence culture, amplification and identification of the rabbit nucleus pulposus cel s in vitro, to observe the growth characteristics of nucleus pulposus cel s in different generations.
METHODS:Type Ⅱ col agenase digestion method plus explants culture method was used to isolate and purify nucleus pulposus cel s and then amplify in vitro. The morphology and growth of primary and passaged cel s was observed under the inverted microscope, the number of cel s was counted and the growth curve was draw. The morphology of the cel s was observed under light microscope after hematoxylin-eosin staining, and the expressions of col agen type Ⅱ and aggrecan were examined by immunocytochemistry.
RESULTS AND CONCLUSION:Nucleus pulposus cel s of rabbit were isolated, cultured and amplified in vitro successful y. Growth activity was observed, and found that the 1-3 generation nucleus pulposus cel s proliferated more rapidly and vigorously. The proliferation of nucleus pulposus cel s was decreased while the cel passaged more generations. These isolated and cultured nucleus pulposus cel s could positively express the col agen type Ⅱ and aggrecan. In vitro combination of type Ⅱ col agenase digestion method and explants culture method could obtain high purity nucleus pulposus cel s, and the cultured nucleus pulposus cel s were grew in round or polygonal. The 1-3 generation of cel s had strong activity.

Objective To explore the clinical application of perforating branch flap of medial vastus muscle. Methods Perforating branch flap (muscle branch) of medial vastus muscle was designed by using the surface projection of the medial vastus muscle artery as flap anxial line and the given point of direct cutaneous artery as flap center to repair skin and soft tissue defects of the knee in seven patients.Results All flaps survived well with primary healing in all patients, with one stage healing. After a follow-up for 1-18 months, all flaps turned out to be with good texture and satisfactory appearance and function of the flaps. Conclusion The surgery of perforating branch flap of medial vastus muscle is simple,safe and easy handling and provides a new feasible surgical procedure to repair skin and soft tissue defects of medial femur and around the knee.

0.05).Histopathologic examination showed no changes in the right gastrocnemius muscles injected with BTXA gel,but ultrastructurally the myopathic changes were clearly visible,like diffuse sarcomere disruption and saroplasmic reticulum expanding.The myofibre degeneration showed no remission 12 months after BTXA gel injection.Conclusion BTXA is dissolved in gel evenly.The long-lasting myofibre degeneration in BTXA gel paralyzed muscles may reflect that the paralyzed muscles fail to regain their unique function and recovery of muscle contraction.

Objective:To explore the change of serum interleukin 16 (IL-16) level and the effects of highly-active antiretroviral therapy (HAART) on IL-16 in patients with HIV infection.Methods:77 patients with HIV infection were studied,with fifteen normal subjects studied as controls.The patients were subdivided into stages according to the standards by US Centers for Disease Control and Prevention (CDC) and the World Health Organization (WHO).Of all the individuals,the CD4+T cells and CD8+T cells and the amout of IL-16 were measured at each stage to find the difference among normal and groups of the patests,and between the groups with and without HAART.Results:The level of CD4+ T cells in the experimental group were lower than that of the control group in of the patients (P