1.Recent advances in surface modification of magnetic iron oxide nanoparticles and their applications for CTCs separation
Chinese Journal of Clinical Oncology 2014;(1):56-59
Magnetic iron oxide nanoparticles (MIONs) have been widely used in biomedical fields, including targeted drug deliv-ery, magnetic resonance imaging, separation and enrichment of cancer cells, and tumor-targeted therapy. However, MIONs are increas-ingly necessary to ensure reduced toxicity, more stable colloidal dispersion, better biocompatibility, and higher magnetic responsive-ness. Thus, apart from the continuous improvement of material synthesis, specific surface modification of MIONs is essential to choose appropriate materials. This paper reviews the available methods and materials and their function in MION surface modification as well as their applications for the separation of circulating tumor cells.
2.Antibacterial mechanisms of common encountered metals:research progress
Mayu SUN ; Yupeng JIN ; Hengyi XU
Chinese Journal of Pharmacology and Toxicology 2016;30(4):415-420
Metals,due to their unique and excellent antibacterial properties,have been widely used in biomedical fields. However,their antibacterial mechanisms are not yet completely clear,so that studies on metal toxicity to bacteria have been a frontier in recent years. There are two main antibacterial mechanisms that have been reported so far. One is based on oxidative damage to bacte?ria induced by the reactive oxygen species,and the other is based on structural changes induced by the Coulomb attraction. The results of bacterial damage could be due to the combination of the two mechanisms. In this paper,current research progress in antibacterial mechanisms of metal has been discussed,which will technically facilitate further development and applications of antimicrobial metal?lic materials.
3.Research progress on enrichment and isolation of circulating tumor cells using immunomagnetic separation based microfluidic technology
Fulai LI ; Pingfeng SUN ; Xiaolin HUANG ; Fan LI ; Hengyi XU
Chinese Journal of Laboratory Medicine 2016;(1):64-67
Circulating tumor cells ( CTCs ) exists in peripheral blood of patients with a variety of malignant tumors , the accurate detection of CTCs helps early detection of cancer , and can be used to guide individualized treatment of cancer patients , rapid assessment of tumor chemotherapy drugs and detection of tumor recurrence .However , the CTCs in peripheral blood of cancer patients were extremely low , their detection requires efficient and specific enrichment and separation methods .The new microfluidic technology can accurately control the microliquid preciously , combined with the immunomagnetic separation technology , has been applied in detecting CTCs , which can save the blood samples of CTCs , has the advantages of simple operation , fast sorting speed , high specificity and high purity .
4.Research advances in characteristics of biotransport and biotransformation and toxicities of quantum dots in vivo
Pengfei YANG ; Lin YANG ; Huijuan KUANG ; Hengyi XU
Chinese Journal of Pharmacology and Toxicology 2015;(6):1007-1013
As a novel fluorescent nanomaterial,quantum dots (QDs) have a prospect for wide application. However , the adverse effects of QDs have become a concern among and more researchers. The toxic actions of QDs in vivo are closely associated with the biotransport and bio?transformation of QDs,which can be affected by the exposure pathways,exposure dose,surface modification and the particle size. Among them,the exposure pathways can affect the absorption and distribution of QDs in vivo,the exposure dose can affect the metabolism and excretion,thus influencing the distribution of QDs in vivo ,the surface modification can affect the distribution ,metabolism and excretion of QDs in vivo,the particle size can affect the absorption,distribution and excretion of QDs in vivo,and larger QDs are more likely to remain in the body and difficult to remove. QDs can enter the body through the circulatory system,get accumulated and degraded in the liver,kidney and other organs. The degraded products can be excerted through excrement and urine under the metabolism in the liver,spleen and kidneys. In addition,QDs can interact with biological macromolecules in the body,causing DNA damage,affecting the function and gene expression level of the liver,kidney,nervous system and other organs,and resulting in pathological and functional damage to tissues and organs.
5.Application of propofol combined with small dose fentanyl in manual reduction anesthesia in orthopedics
Qianrong WANG ; Hengyi XU ; Huanzhong HE ; Bin GAO
Chinese Journal of Biochemical Pharmaceutics 2017;37(6):254-256
Objective To explore the application of propofol combined with small dose fentanyl in manual reduction anesthesia in orthopedics.MethodsOctober 2014 to August 2016 in Huzhou central hospital treated 120 cases of acute long bone closed fracture and ankle, shoulder dislocation and other patients, efficient analysis anesthesia, surgery time, patient recovery time, propofol is added volume and total volume, and detection 3min, 1min, 3min, 5min and waking SpO2, HR, MAP, RR values.After administration before it is administered.ResultsAnesthetic effect experimental group total efficiency is higher, the difference was statistically significant (P<0.05);after simultaneous administration of all patients 1min, 3min, 5min signs were lower than prodrug 3min signs, the difference statistically significant (P<0.05), after clear signs of its former administration 3min with no significant difference;experimental group recovery time, propofol bolus of propofol than with total control were lower, the difference was statistically significant (P<0.05);and the two groups were similar to the operation time, the difference was not statistically significant.ConclusionIn summary, propofol combined with entanyl anesthesia for patients to take the treatment of orthopedic manual reduction obviously.
6.Biotin-streptavidin system-mediated folate receptor-targeted quantum dot in vitro imaging of ovarian cancer SKOV3 cells
Liju NIE ; Hengyi XU ; Chenlian YE ; Xiaolin HUANG ; Wenting LIU ; Fen FU
Chinese Journal of Clinical Oncology 2014;(19):1254-1258
Objective:To develop a biotin-streptavidin system (BAS)-mediated folate receptor (FR)-targeted quantum dot (QD) fluorescent probe and preliminarily validate the targeting ability and signal amplification effect of the probe. Methods: Streptavidin (SA) was covalently coupled with QD through the active ester method;the physical characteristics of the prepared QD-SA were veri-fied. Biotinylated folate was synthesized through the carrier bovine serum albumin using the same method and then reacted with QD-SA to form the special probe. The probe was used to identify SKOV3 cells and FR-negative A549 cells to verify its targeting speci-ficity. QD-SA was used as the contrast. SKOV3 cells were imaged using the BAS-mediated FR-targeted QD probe with a biotinylated folate incubation time of 1 or 4 h. Various reaction times were also tested between the probe and the QD-FA that was formed without BAS mediation. Results:The BAS-mediated FR-targeted QD probe specifically recognized FR-positive SKOV3 cells. The probe ob-tained higher fluorescent intensity after 4 h than after 1 h of biotinylated folate incubation. The BAS-mediated FR-targeted QD probe al-so had a stronger fluorescent signal than the QD-FA probe. Conclusion:The proposed probe presents a great potential in the early diag-nosis of ovarian cancer because of its high specificity and sensitivity.
7.Construction of C-shaped cartilage with electrospun gelatin/polycaprolactone nanofibrous membranes and 3D printed supporter
Hengyi ZHANG ; Bei FENG ; Yang GE ; Manchen GAO ; Jie BAI ; Wei FU ; Zhiwei XU
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(7):896-900
Objective·To construct C-shaped cartilage rings by rabbit auricular cartilage-derived chondrocytes combing with both electrospun gelatin/ polycaprolactone(GT/PCL) nanofibrous membranes and 3D printed supporters for repairing tracheal cartilage defects.Methods·Primary chondrocytes were isolated from rabbit auricular cartilage with methods of trypsin enzyme digestion and collagenase enzyme digestion.After proliferation in vitro,the chondrocytes of passage 2 were harvested for further experiments.Ultrafine composite fibers of GT/PCL were fabricated via electrospinning.The electrospun GT/PCL membranes were tailored into rectangle shape,the length of which is 12 cm and the width is 2.5 cm.Chondrocytes were seeded on membrane at a density of 1 × 108 cells/mL.Then the membrane were rolled onto a 3D printed supporter of poly(DL-lactide-ε-caprolactone) (PLCL) material to construct a C-shaped cartilage-like complex.After 8 weeks of subcutaneous incubation in vivo,gross inspection and paraffin section staining were applied for evaluation.Results·After 8 weeks of culture in vivo,mature cartilage-like tissue were formed with open-cylindrical bellow appearance and pecific mechanical property.C-shaped rings arranged at regular intervals on the inner surface of tissue,which were similar to the normal structure of tracheal cartilages.Histological and immunohistological staining showed a large number of typical lacunar structures and extracellular matrix secretions.Conclusion·It is feasible to construct tissue engineered C-shaped cartilage tissue by combing chondrocytes with GT/PCL membrane and 3D printed PLCL supporter for tracheal cartilage repair.
8.Cloning, expression and functional analysis of the genes in TPS/TPP trehalose synthetic pathway of Meiothermus ruber.
Yueming ZHU ; Yichen TANG ; Hengyi XU ; Juan ZHANG ; Dongsheng WEI ; Laijun XING ; Mingchun LI
Chinese Journal of Biotechnology 2009;25(3):399-405
By constructing the genomic DNA library of Meiothermus ruber CBS-01, the genes of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) involved in trehalose synthesis were cloned. The genes were cloned into the plasmid pET21a, and expressed in Escherichia coli Rosetta gami (DE3). The activities of these two purified enzymes were confirmed by thin layer chromatography (TLC). Meanwhile, we tested the cellular compatible solutes of M. ruber CBS-01 under different environmental pressure, and found that under hyperosmotic pressure, this strain can accumulate trhalose-6-phosphate, but not trehalose. These results can give more insight to future research in the roles of TPS/TPP and TreS pathway.
Bacterial Proteins
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genetics
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metabolism
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Cloning, Molecular
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Escherichia coli
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genetics
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metabolism
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Glucosyltransferases
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genetics
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metabolism
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Phosphoric Monoester Hydrolases
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genetics
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metabolism
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Recombinant Fusion Proteins
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genetics
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isolation & purification
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metabolism
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Thermus
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enzymology
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genetics
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Trehalose
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biosynthesis
9.Provincial distribution differences and influencing factors of self-assessed health among elderly population in China
Hengyi XU ; Qin YANG ; Ting CHEN
Journal of Environmental and Occupational Medicine 2024;41(2):193-199
Background Regional differences in economic development, natural environment, health care level, and social structure may lead to differences in the provincial distribution of the health status of the elderly population. Objective To explore the provincial distribution characteristics, regional differences, and influencing factors of the self-assessed health of the elderly population, with the aim of providing a policy basis for improving the health of the elderly population and promoting healthy aging according to local conditions. Methods Using 31 provinces (municipalities and autonomous regions) in China as the basicstudy unit and based on the method of Wagstaff, the self-rated health data of the elderly population (aged 60 years and above) in each province from the 2010 and 2020 national censuses and the 2015 1% National Population Sample Survey were converted into ill-health scores as a measure of self-assessed health, and higher scores represented worse health status perception. Global Moran's I was used to evaluate spatial autocorrelation, range [−1, 1], with a value of 1 as a perfect clustered pattern. Local Moran's I was used to evaluate the tendency of local autocorrelation, and high-high aggregation/low-low aggregation indicated that both target province and its neighboring provinces showed higher/lower ill-health scores. Spatial econometric models were selected by Lagrange multiplier test and Hausman test to explore influencing factors of the self-assessed health of the elderly population. Results In 2010, 2015, and 2020, the national ill-health scores of the elderly population were 1.831, 1.873, and 1.547, respectively, and the corresponding Global Moran's I statistics were 0.347, 0.482, and 0.511, respectively (P<0.01), indicating that the ill-health scores of the elderly population showed a significant spatial positive autocorrelation, and the degree of spatial aggregation was increasing gradually. From 2010 to 2020, the high-high aggregation of ill-health scores among the elderly population was concentrated in the inland northwest, while the low-low aggregation was concentrated in the southeast coast, gradually showing a "southeast-central-northwest" stepped incremental pattern of differentiation. The Lagrange multiplier test and Hausman test suggested that the fixed-effects spatial lagged model was a better choice, and the regression model showed a spatial autocorrelation in the ill-health scores of the elderly population, with an autocorrelation coefficient of 0.3969 (P<0.001); the ill-health scores of the elderly population were negatively correlated with the natural logarithms of gross regional product per capita, and the number of beds in health care facilities per 1000 population, with regression coefficients of −0.8297 and −0.0454 (P<0.05) respectively, and positively correlated with the annual average concentration of PM2.5, illiteracy rate, and the number of health technicians per 1000 population, with regression coefficients of 0.0033, 0.0297, and 0.0765 (P<0.05), respectively. Conclusion From 2010 to 2020, the overall self-assessed health level of China's elderly population showed an upward trend and a spatial positive autocorrelation, with better self-assessed health in the southeast coast and poorer ratings in the northwestern inland. Additionally, there was a gradual decline in self-assessed health of the elderly population from the southeast to the central regions and further to the northwest in terms of spatial distribution. Economic development level, environmental pollution, health resource allocation, and education level are important factors influencing the self-assessed health of the elderly population.
10.Research progress of biosensors in the detection of foodborne pathogens.
Fangbin XIAO ; Rui LIU ; Zhongxu ZHAN ; Gan ZHANG ; Xin WU ; Hengyi XU
Chinese Journal of Biotechnology 2019;35(9):1581-1589
As the main factor leading to foodborne illnesses, foodborne pathogens have been attached great importance by people. The development of simple, rapid, high-sensitivity and low-cost food-borne pathogen detection methods is of great significance in reducing the incidence of foodborne diseases. Biosensor technology is a new micro-analysis technology developed by multi-disciplinary cross-infiltration. It has the characteristics of high sensitivity and fast analysis speed, and is widely used in the detection of food-borne pathogens. This paper introduces the basic principles of biosensors, summarizes the application of common biosensors in the detection of foodborne pathogens, and prospects for future development.
Biosensing Techniques
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Food Microbiology
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Foodborne Diseases
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Humans