1.Application of quantum dots immune fluorescent labelling in colorectal cancer tissues
Fengping HE ; Xin XU ; Qingsong WU ; Dingyun LI ; Zhanzhong MA ; Sheng TANG ; Weidong YIN ; Haitao GONG ; Hengxian LIN
International Journal of Laboratory Medicine 2016;37(18):2531-2533
Objective To investigate different antigens detected by a novel labelled reagent‐quantum dots(QDs) in the colorectal cancer tissues microarray(TMA) .Methods Depend on QDs streptavidin conjugate(QDs‐SA) combined specially with biotinylation IgG ,immune of luorescent histochemistry was utilized to examine expression of K‐ras ,matrix‐remodeling associated 5(MXRA5) proteins in the colorectal cancer TMA ,where the protein accurate location was observed .Results K‐ras ,matrix‐remodeling associ‐ated 5(MXRA5) proteins were high expressed in colorectal cancer tissue and located accurately in the cell membrane and nucleus of colorectal cancer cells ,respectively .Conclusion QDs exhibit excellent photostability ,broad emission spectrum and long fluorescence lifetime .Modified with streptavidin could accurately detect different protein locations in the colorectal cancer TMA .This is a novel approach for studying targeted imaging of colorectal cancer in vivo and vitro clinical diagnosis .
2.Clinical application of real-time fluorescence quantitative PCR for the detection of fecal tumor M2-pyruvate kinase in colorectal cancer patients
Yulan LIU ; Fengping HE ; Xin XU ; Qingsong WU ; Dingyun LI ; Zhanzhong MA ; Yanle GUO ; Sheng TANG ; Weidong YIN ; Haitao GONG ; Yi LIU ; Hengxian LIN
International Journal of Laboratory Medicine 2017;38(11):1444-1446
Objective To investigate the application valve of real-time fluorescence quantitative polymerase chain reaction(RT-PCR) for the detection of tumor M2-pyruvate kinase(tM2-PK) DNA in patients with colorectal cancer(CRC).Methods Fragment of tM2-PK DNA(162 bp) was amplified and inserted into PGM-T vector to construct recombinant plasmid,which was used to develop RT-PCR method.Sensitivity,specificity and repeatability of RT-PCR for the detection of tM2-PK were analyzed.From Jan.2014 to Jun.2016,200 CRC patients and 100 healthy subjects were enrolled and detected for fecal and serum tM2-PK DNA by using RT-PCR,and the detected results were compared with those detected by using enzyme linked immunosorbent assay(ELISA).Results Recombinant plasmid was successfully constructed,which was certified by sequencing.The sensitivity of RT-PCR for the detection of tM2-PK DNA was 10 copy/mL,with high specificity and 0.3%-2.9% of coefficient of variation.In patients,the positive rate of fecal tM2-PK DNA,detected by RT-PCR,was 92.50%,and that of ELISA to detect tM2-PK was 80.00%.Fecal and serum levels of tM2-PK were correlated with the pathologic stages of tumour.Conclusion Self-established RT-PCR could be specificity and sensitivity for the detection of fecal tM2-PK,which could be used for the early diagnosis of CRC.
3.Investigation of a family clustering of human infection with avian influenza A (H7N9) virus in Nanning, Guangxi.
Hai LI ; Mei LIN ; Email: GXLINMEI@126.COM. ; Zhenzhu TANG ; Xinqin LIN ; Yi TAN ; Minmei CHEN ; Haojie ZHONG ; Haiyan LIU ; Fuyin BI ; Jianyan LIN ; Shuwu ZHOU ; Zhuoxin HUANG
Chinese Journal of Epidemiology 2015;36(5):481-483
OBJECTIVETo understand the transmission mode of human infection with avian influenza A (H7N9) virus.
METHODSField epidemiological investigation was conducted for a family clustering of human infection with H7N9 virus in Hengxian county, Guangxi Zhuang Autonomous Region in February 2014. Two patients and their 82 close contacts were surveyed. The samples collected from the patients, environments and poultry were tested by using real time reverse transcriptase-polymerase chain reaction (rRT-PCR), and the samples from patients were used for virus isolation. The samples from 5 close contacts were tested with RT-PCR. The clinical data, exposure histories of the patients and the detection results of the isolates and their homology were analyzed.
RESULTSPatient A became ill 4 days after her last exposure to poultry in Zhongshan, Guangdong province, and returned to her hometown in Hengxian 2 days after onset. Patient B was patient A's 5 years old son, who had no known exposure to poultry but slept with patient A for 4 days. He developed symptoms 4 days after last contact with his mother. Two strains of H7N9 virus were isolated from the two patients. The 2 isolates were highly homogenous (almost 100%) indicated by gene sequencing and phylogenetic tree. None of the other 81 close contacts developed symptoms of H7N9 virus infection.
CONCLUSIONPatients B was infected through close contact with patient A, indicating that avian H7N9 virus can spread from person to person, but the transmissibility is limited and non-sustainable.
Animals ; Child, Preschool ; China ; Cluster Analysis ; Contact Tracing ; Family ; Female ; Homozygote ; Humans ; Influenza A Virus, H7N9 Subtype ; genetics ; isolation & purification ; Influenza, Human ; transmission ; virology ; Male ; Phylogeny ; Poultry ; virology ; Real-Time Polymerase Chain Reaction ; Sleep