Objective: To construct a lentiviral expression vector that inhibits enhanced green fluorescent proteins(EGFP).Methods: The EGFP shRNA was cloned into the pLL3.7 lentivirus vector digested with XbaⅠ/XhoⅠ.J558L and Hela cells were infected by lentiviruses,and the EGFP expression was observed with the fluorescence microscope.Results: The EGFP RNAi sequence was correctly cloned into the lentivirus vector with the U6 promoter,and the EGFP expressions of Hela and J558L cells were suppressed after infected by lentivirus containing EGFP shRNA. Conclusion: The lentivirus vector effectively inhibited EGFP expression,which could be used to mediate further researches on gene function.

Objective To investigate the type of gene mutation and its distribution in patients with thalassemia in Chongqing ,in order to guide prenatal and postnatal care .Methods PCR and membrane hybridization technology for α-thalassemia andβ-thalasse-mia gene detection .Results From September 2012 to September 2013 ,349 thalassemia patients were detected ,including 125 α-thalassemia patients and 211β-thalassemia patients .Based on genotypes ,gene deletions were the most common type of gene muta-tion of α-thalassemia patients .Southeast Asia deletion - -SEA/αα(accounted for 73 .60 % ) and the right deletion -3 .7/αα(ac-counted for 19 .20% ) were the major types of gene deletion .Forβ-thalassemia patinets ,the hot spots of mutation were CD17(A→T) ,CD41-42(-TCTT) and IVS-Ⅱ-654(C→ T) ,which accounted for 29 .38% ,28 .91% and 27 .49% respectively .α-thalassemia combined with β-thalassemia mutations were detected in 13 patients .Conclusion Studing the gene mutation types of thalassemia and its distribution provides valuable information for genetic counseling and clinical therapy in Chongqing .

Objective To study the asymmetric dimethylarginine(ADMA) concentrations in plasma of acute coronary syndrome (ACS) and its various subgroups ,and analyze the relationship between ADMA and traditional risk factors of ACS .Methods 101 ACS patients were enrolled in the study ,which were divided into several subgroups according to clinical diagnosis ,ECG and coro‐nary angiography .ACS group were divided into unstable angina(UA) group(n=70) and acute myocardial infarction(AMI) group (n=31) ,then AMI group was divided into non‐ST segment elevation myocardial infarction(NSTEMI) group(n=19) and ST seg‐ment elevation myocardial infarction (STEMI) group(n=12) .The control group(n= 40) were recruited in the study during the same period ,who showed normal coronary arteries in coronary angiography .All the patients received coronary attery angiography . The plasma ADMA concentration was measured by using enzyme‐linked immunosorbent assay (ELISA) ,and serum contents of lip‐id(TC ,TG ,HDL‐C ,LDL‐C) ,C‐reactive protein(CRP) ,troponin were detected by automatic biochemic analysis apparatus .Results Compared with the control group ,plasma ADMA concentration of ACS group increased ,the difference was statistically significant (P<0 .05) .The concentrations of ADMA in AMI group and UA group were higher than those in control group ,and the difference was statistically significant(P< 0 .05) .The concentrations of ADMA in AMI group were significantly higher than those in UA group(P<0 .05) .Plasma ADMA negatively correlated with HDL‐C concentrations(r= -0 .222 ,P<0 .05) ,positively correlated with LDL‐C concentration(r=0 .357 ,P<0 .05) ,and positively correlated with CRP(r=0 .619 ,P<0 .05) .Conclusion The occur‐rence of ACS and some of its related risk factors are correlated with plasma ADMA concentration .