Objective To investigate whether preemptive intrathecal administration of CRH has an antinociceptive efficacy on inflammatory pain model rats. Methods The antinociceptive efficacy of preemptive intrathecal administration of CRH was evaluated by the flinch-licking time and the tail flicking latency in formalin-subjected pain model rats. The c-fos gene expression in spinal cord dorsal horn was analyzed by immunohistochemical technique and computer image technique. Results Comparing with NS control group, preemptive intrathecal administration of 0.5 ?g CRH could markedly inhibit the phase Ⅱpain reaction and meanwhile decrease c-fos gene expression by 25.02% in the superficial lamella of spinal cord dorsal horn of the model rats. The antinociceptive efficacy could be counteracted by preemptive intraperitoneal injection of CP-154526 (the specific antibody of CRHR1, 10 mg/kg). Conclusion CRH could significantly inhibit pain reaction and spinal cord c-fos gene expression, and thereby preemptive intrathecal administration of CRH will contribute to the antinociceptive efficacy because of its potency of inhibiting hyperalgesia.

Objective To study the influence and mechanism of seawater immersion on endothelial cell injury sustained by burn firearm combined injury to improve the early therapeutic efficacy. Methods The dogs with burn firearm combined injury were randomly divided into two groups: immersion group and control group. In immersion group, the dogs were immersed in seawater for 4 hours, then taken out from seawater. Blood samples were collected from central vein at 4 h, 7 h, 10 h, 20 h and 28 h following wound for the detection of changes of the circulating endothelial cells (CEC) and von Willebrand Factor (vWF). The same procedures except immersion were performed in the control group. Results The levels of CEC and vWF elevated at 4 h and 7 h following wound in control group( P

Objective To investigate the effect of seawater immersion on the hemostatic function of endothelial cells in dogs sustained burn firearm combined injury and the mechanisms. Methods Dogs with burn firearm combined injury were randomly divided into two groups: immersion group and control group. Dogs in immersion group were immersed in seawater for 4 h, and then taken out from seawater. Blood samples were collected from central vein before wound, immediately after immersion, at 4, 7, 10, 20, and 28 h after immersion to detect the changes in circulating endothelial cells (CEC), tissue type plasminogen activator (t PA), plasminogen activator inhibitor (PAI), and thromboxane B 2/6 keto prostaglandin F 1? (TXB 2/6 K PGF 1? ). Dogs were sacrificed at 28 h after wound for the purpose of pathological examination of the lung tissue. The indices detected except seawater immersion in the control group were the same as those in the immersion group. Results In the control group, the levels of CEC, PAI 1, and TXB 2/6 K PGF 1? increased, but the level of t PA decreased at 4 and 7 h after wound. However, in immersion group, the levels of CEC, PAI 1 and TXB2/6 K PGF1? kept increasing, but the level of t PA kept decreasing at 4, 7, 10, 20, and 28 h. Each index in immersion group from 4 h to 28 h after wound was significantly different from that in the control group ( P

0 01) in the two groups were significantly different Conclusion After CPR in non hemorrhagic CA, quick infusion of polyglucose and sodium chloride solution is beneficial to the recovery of erythrocyte rheological parameters before CPR

Objective　To investigate the effect of cardiopulmonary bypass (CPB) on vascular endothelial cell injury and plasma endothelin-1 and nitric oxide equilibrium in patients undergoing cardiovascular operation with CPB. Methods　A total of 20 patients with congenital heart disease (Group Ⅰ) and 20 with valvular problem (group Ⅱ) were operated on under CPB respectively. Blood samples were collected from central vein before skin incision, before CPB, 30 min after CPB, at the end of CPB, and end of operation, the first morning and third morning after operation. The levels of plasma thrombomodulin(TM), endothelin-1(ET-1) and nitric oxide(NO) were measured. Results　The plasma TM level was significantly elevated during CPB (P<0.01, P<0.05) and 1 d after operation, reached its peak as (4.88±1.12) ng/ml in Group Ⅰand (8.34±1.84) ng/ml in group Ⅱ at the end of surgery and came back to the level as before operation. The plasma level of ET-1 was also increased significantly after CPB and reached peak as (129.04±22.29) in Group Ⅰ and (156.62±29.66) in Group Ⅱ at the end of operation. And the level was still higher than before operation in 2 groups 3 d after operation. No change was found on the level of NO in 2 groups. Conclusion　CPB may cause extensive acute endothelial cells damage for about 24-48 h and recovered about 72 h and it may also cause an imbalance of ET-1 and NO.

Objective To assess the changes of coagulation and fibinolysis in patients undergoing thoracotomy. Methods Forty patients undergoing thoracotomy were enrolled into this trial. The venous blood were collected for normal coagulable function test at the following time points: before surgery, at 3 h after the beginning of surgery, at 1 d and 3 d after surgery. Results PT, APTT levels had no obvious change. Compared with before and during surgery, FG levels decreased obviously at 1 d and 3 d after surgery. TEG showed the value of R and K increased during and after surgery and value of MA decreased during and 1 d after surgery, but they had no significance in statistics. CI decreased significantly during and after surgery as compared with before surgery(P

Objective To approach the relationship between visceral histopathology and blood bacteriology changes of projectile-burn combined wound jointed with seawater soak. Methods After model creation in dogs of projectile-burn combined wound jointed with seawater soak, fifteen dogs were randomly assigned randomized into two groups: projectile-burn-only(unsoaked group, US), projectile-burn combined wound with seawater immersion(soaked group,S). 0, 4,7,10,20,28h after injury the blood samples were taken for bacterial culture. Tissue samples of heart, liver, kidneys were taken for pathological examination with light microscope (LM) 28h after injury or in the moribund stage. Results Bacteriemia was found in soaked group earlier and severer.The bacteriemia has a multiple sources. There were not only intestinal bacteria, but also surface bacteria and seawater bacteria found in soaked group. Different degrees of circulation disturbance, degeneration changes and severe inflammation reaction were found in viscera. Conclusion After seawater soak, bacteriemia aggravated the injury of animals′ viscera, and thus led to a higher mortality.

Objective To investigate the effect of ondansetron on the development of neuropathic pain in rats following chronic constriction injury (CCI) on sciatic nerve. Methods This research consisted of 3 parts. ①A single bolus of ondansetron was given intraperitoneally 2 h before nerve injury; ②A single bolus of ondansetron was given intraperitoneally on the 7th day after nerve injury; ③Continuous administration of ondansetron was given daily for 35 d; In each part, male SD rats were randomly divided into ondansetron group (0.5, 2, 8 mg/kg), saline group and sham operated group. The paw withdrawal response to thermal stimulation was tested throughout the 35 d following nerve injury. Results Ondansetron administration before nerve injury had no effect on thermal pain threshold in rats of experimental neuropathic pain. ondansetron administration on the 7th day after nerve injury attenuated the descending of thermal pain threshold but did not alter its descending trend in subsequent time period. Daily administration of ondansetron for 35 d attenuated and further delayed the descending of thermal pain threshold significantly in a dose-dependent manner. Conclusion Continuous administration of ondansetron can prevent or at least delay the descending of thermal pain threshold of rats with experimental neuropathic pain after constriction injury.

Objective To study vascular endothelial cell injury/activation associated with CPB in order to understand the pathophysiology of the complications of patients undergoing cardiovascular operationsMethods Twenty-six patients scheduled for cardiac operations with CPB, were divided into two grourps, group Ⅰ: 14 patients with acyanotic congenital heart diseases undergoing corrective surgical procedures; group Ⅱ: 12 patients undergoing selective cardiac operation for valvular replacement Blood samples were withdrawn from central vein before skin incision,before CPB, 30 min following CPB, at end of CPB, at end of operation, on the first postoperative day and the third postoperative day to measure the levels of circulating endothelial cells(CEC), thrombomodulin(TM) and von Willebrand Factor(vWF).Results The levels of CEC,TM and vWF significantly elevated during CPB and on the first postoperative day in the two groups,as compared with those before operation (P

Objective To investigate the changes in counts of T-lymphocyte subsets, natural killer cell (NK cell) and the levels of serum soluble interleukin-2 receptor(sIL-2R) after perioperative allogeneic whole-blood transfusion or erythrocyte suspention (SAG-M blood) blood transfusion. Methods Thirty patients undergoing elective refection for rectal cancer, were randomly allocated to receiving 400 ml of allogeneic whole-blood (n = 15 ) or 400 ml of SAG-M blood (n = 15 ) during perioperative period, respectively. Indirect immunofluorescence technique and enzyme-linked immunosorbent assay were used to determine the counts of T--lymphocyte subsets, NK cells and to detect levels of serum sIL-2R 1 day and 5 day after operation.Results The counts of CD3 +, CD. + and NK cells in both groups decreased significantly after operation (P