Four bdellovibrio bacteriovorus of Rhizobium japonicum were isolated in our country from field soils cropped to soybeans. The culture character, morphology, host range and the other biological properties of these bdellovibrio bacteriovorus of R. japonicum had a wide host range and a strong ability that lyse R. japonicum in field soils, and were one of main biological factors that lower the yield of soybeans.

Objective:To discuss the management and maintenance of medical equipment in hospital, the department of biomedical and engineering should develop a reasonable management plan to meet the needs of using safety management of medical equipment based on the risk analysis.Methods: Make up a strategy based on the analysis of application risk according to the clinical function, physical risk, fault avoidable probability and the adverse events recorded, and conduct the preventative maintenance and safety performance inspection. Results: The focus of clinical engineering professionals change from passive acceptance to repair equipment to preventive equipment maintenance, and carry out safety performance inspection to reduce the risk caused by the failure of equipment which is harmful to the patients.Conclusion: The clinical engineering professionals need to pay attention to the application risk and improve the management strategy while they improve the technical level.

To observe the patients with type 2 diabetes who were injected premixed insulin analogues after short time continuous subcutaneous insulin infusion( CSII ) treatment.It was found that the patients needed premixed insulin analogue injection 2 times daily when the dosage of CSII was less than 45 U/d,and 3 times daily when the dosage was greater than 45 U/d.The relationship of dosages between premixed insulin analogue treatment ( Y ) and CSII treatment(X) was calculated by following equations:2 times daily premixed insulin analogue treatment:Y =13.093 +0.395X,3 times daily premixed insulin analogue treatment:Y =23.114+0.405X.

Objective To investigate the interaction between glucose transporter 4(GLUT4) and the(14-3-3) proteins in rat adipocytes.Methods Isolated primary adipocytes were obtained from the epididymal fat pads of male Sprague-Dawley rats by collagenase Ⅰ digestion.Total cell extract as well as three fractions,referred to as Fraction T,H and L,were obtained from rat adipocytes using hypotonic lysis and glycerol gradient velocity sedimentation.Immunoadsorption of GLUT4 with Trisacryl beads cross-linked with 1F8,a specific monoclonal antibody to GLUT4,was conducted in total cell extract and the three fractions and followed by elution and Western blot analysis.Co-immunoprecipitation of(14-3-3) proteins and GLUT4 was demonstrated by detecting(14-3-3) in the eluates.Results(14-3-3) proteins were co-immunoprecipitated with GLUT4 either in the total adipocyte extract, or in each of the three fractions mentioned above.The co-precipitated(14-3-3)s were identified as two bands in Western blot analysis,the molecular weights of which were approximately 29kDa and 60kDa respectively,indicating that they might be in the state of homo-or hetero-dimers while interacting with GLUT4.Conclusion There is a physiological interaction between(143-3) proteins and GLUT4 in rat adipocyte.

Objective: To analysis the key factors of quality control for high frequency surgical unit to provide reference to ensure accurate and standard. Methods:To analysis the details of quality control on protective earth impendence, resistor-capacitor network, test error on definition, structure, magnitude and other aspects. Results:To master the key factors of quality control is important for the promotion of operability of the quality control work and decrease the using risk, and then to improve the quality of medical services ensure the safety of patients and medical staff. Conclusion: To analysis the each key factor is important significance to the quality control for high frequency surgical unit.

Objective:To master the methods of reducing electromagnetic interference by RFID system, and presents the feasible technical advices for hospital’s implementation to ensure the safety of applications of electronic medical devices. Methods:According to analysis the causes of electromagnetic interference by RFID system, the methods of suppressing the electromagnetic interference are put forward for practical application. Results:The clinical engineers could refer to the methods of suppressing the electromagnetic interference and then conduct and use the RFID system, taking account of the management efficiency and the safety of applications of the medical devices. Conclusion:To determine the proper strategy for RFID system application in medical management of hospital may reduce the electromagnetic interference to the electrical devices of hospital and ensure its safety of application.

Objective Valsartan, the angiotensin Ⅱ type 1 receptor blocker, is recently proved to reduce urinary albumin at the microalbuminuria stage in human diabetic nephropathy without altering glucose metabolism. But the pathway is still uncertain. In present study, we examined the changes of adrenomedullin receptor (ADMR) mRNA and protein expressions in the renal cortex of diabetic rats to investigate the protective effects of valsartan on an experimental model of diabetic renal injury. Method The SD rats were randomly divided into following groups: normal rats, STZ-induced diabetic rats, and diabetic rats treated with valsartan. STZ-induced diabetic rats were treated with valsartan (10mg/kg body weight) or vehicle for 8 weeks. The expressions of ADMR mRNA in renal cortex were analyzed by RT-PCR, as well as ADMR protein expressions were detected through western blot. Results We found (1) Valsartan treatments reduced urinary albumin excretion in 24h, compared with the untreated. But no notable difference was seen in HbA1c and blood sugar of diabetic rats between the two groups. (2) Valsartan treatments increased the expressions of ADMR mRNA and protein in diabetic rats renal cortex. Conclusion These results indicate that valsartan treatment can upgrade the expressions of ADMR in the renal cortex of diabetic rats. It may be one of renal protective pathways of Ang Ⅱ type 1 receptor blocker.

Within the KABP model as a framework,the authors studied these behaviors at the following three levels,namely knowledge and learning,beliefs and attitude,and behavior.Such aspects as the education background and training,drug expertise,drug infonmation sources,personal experience and habits,attitudes,needs of physicians and patients',socio-demographic characteristics were discussed among others to probe into how physicians' personal behavior affects their prescription behavior.Recommendations raised include education and training,provision of correct drug information,greater publicity,better compensation mechanism of public hospitals,strengthened supervision,establishment of the system of prescription audit,etc.

Purpose:To study the effectiveness of surgical treatment of 15 tracheoesophageal fistulas secondary to carcinoma of esophagus a fter radiotherapy. Methods:clinical records of 15 tracheoesophageal fistulas secon dary to carcinoma of esophagus after radiotherapy were analysed.several surgical techniques were analyged for different cases. Results:In all 15 cases, only two of them died,the postoperativ e death rate is 15.38%, and the total complication rate is 30.76%.1-year survi val is 50%, and the median-life span is 12 months. Conclusions:Approriate surgical treatment together with active preoperative preparation, elaborative postoperative nursing care, can improve t he quality of life, reduce postoperative complication rate, and prolong survival .

Ultraviolet (UV) is a kind of important environmental factor that can cause dermatitis,photoaging and skin cancerization.The UV-induced production of inflammatory factors by epidermal keratinocytes has been deemed an important molecular event in occurrence of the above diseases.Various receptors,intracellular signal transduction-related regulatory molecules and nuclear transcription factors are involved in UV-induced inflammatory responses in keratinocytes,which mainly include epidermal growth factor receptor,aryl hydrocarbon receptor,peroxisome proliferatoractivated receptor,protein kinase C family,protein kinase B,mitogen-activated protein kinase family,nuclear factor-κB and transcription factor activator protein-l,etc,and constitute a complex inflammatory signal transduction network.