Objective: To study the protective effects of L-Carnitine(LC) against the kidney damage induced by cisplatin(DDP).Methods: 36 Female adult Wistar rats were randomly divided into Control group,DDP group and LC+DDP group.Control group and DDP group were fed a basic diet,while LC+DDP group were added 1%LC.After 7 days,DDP group and LC+DDP group received cisplatin i.p.(5 mg/kg body weight),while Control group received normal saline i.p.After 4 days,rats were killed,the change of serum Cr and BUN,and tissue MDA and GSH in kidney were measured and the kidney structure was also observed.Results: Compared with DDP group,the contents of serum Cr、BUN and kidney tissue MDA of LC+DDP group declined very significantly,while the contents of kidney tissue GSH increased significantly.The pathological slice indicated that the renal structure of LC+DDP group was significantly improved.Conclusion: Dietary supplementation with LC might protect kidney against the damage induced by cisplatin in some extent,which might be partially correlative with the antioxidative effects of LC.

Objective To compare the distribution patterns and analyze the antimicrobial resistance of pathogens of different clinical samples .Methods All pathogens were isolated from clinical specimens(blood ,urine and wound) ,the distribution of patho-gens and drug resistance were monitored continuously from October 2012 to October 2013 in Daling hospital .Results 279 strains weredetectedfrombloodculture.Thedistributionincidenceof Escherichiacoli,Klebsiellapneumoniae,coagulasenegativeStaphy-lococci(CNS) ,Staphylococcus aureus ,Enterococcus f aecalis were 28 .67% ,12 .19% ,13 .62% ,9 .68% and 8 .60% respectively .574 strains were detected from urine culture .The distribution incidence of Escherichia coli ,Candida ,Pseudomonas aeruginosa ,K leb-siella pneumoniae ,Enterococcus f aecalis were 38 .68% ,18 .64% ,7 .84% ,7 .49% and 3 .66% respectively .292 strains were detected from wound secretion .The distribution incidence of Staphylococcus aureus ,Escherichia coli ,CNS ,Pseudomonas aeruginosa ,En-terococcus f aecalis were 17 .12% ,14 .38% ,10 .27% ,7 .53% ,5 .48% respectively .Conclusion Clinical using of antimicrobial agents should monitor drug resistance and consider various factors .

Chimeric antigen receptor T cells (CAR-T) therapy is progressing rapidly, and its safety has been widely concerned. At the 62nd American Society of Hematology Annual Meeting, a series of reports on the mechanism, predictive indicators and treatment strategies of major adverse reactions were carried out. These reports have certain guiding significance for comprehensively improving the safety of CAR-T therapy.

Objective To assess the effect of chloramine disinfection on the formation of drinking water disinfection by-products(DBPs )and their mutagenicity.Methods The bacterial indices,chloroform and carbon tetrachloride content in the finished water samples using liquid chlorine and chloramine disinfection respectively were determined in August,2001(plentiful water season)and in March,2002(low water season).The mutagenicities of organic extracts from the water samples were tested using Ames test.The levels of DBPs and the mutagenic activities of water samples treated with different disinfection methods were compared.Results The total count of bateria and coliform bacteria were0/ml and0/L respectively at free chlorine con-centration of about 1mg /L in the chloraminated drinking water,which decreased81%-84%compared with those of the chlori-nated drinking water samples.The carbon tetrachloride concetration were all

[Objective]To investagate the anatomic features in the approach to the brachial plexus in neck in order to make the exposure of brachial plexus more convenience and safer.[Method]Neck of six embalmed adult cadavers were made serial sectional specimens,which was observed to identify the border,composition and anatomic relation of fat layer between the superficial and deep layer of cervical fascia in the posterior triangle of neck with the brachial plexus.[Result]There was no branches of superficial cervical plexus in the fat layer between the superficial layer and deep layer of cervical fascia below the middle neck,in which there was no important structures except omohyoid muscle and transverse cervical artery.The deep layer of cervical fascia was dense and was directly superficial to the brachial plexus.[Conclusion]Exposure of brachial plexus in the neck would be more safe and convenience upon the understanding of anatomic relatiowship between cervical fascia and brachial plexus.

AIM: To investigate the role of adrenomedullin (AM) in diabetic nephropathy. METHODS: We observed the changes in the expression and secretion of AM, TGF-? 1 in the cultured human mesangial cells under high glucose condition and the contents of the laminin and type IV collagen in the supernatants. The effect of intervention with AM was also observed. RESULTS: High glucose condition resulted in increase in the expression and secretion of AM TGF-? 1 laminin and type IV collagen. AM reversed the influence of high glucose on the cultured human mesangial cells. CONCLUSION: These results showed that high glucose condition is one of stimulating factors of AM and the renal protective action of AM may be associated with suppression of TGF-? 1 and reducing excessive accumulation of laminin and type IV collagen.

Animals ; Dibutyl Phthalate ; toxicity ; Female ; Hippocampus ; cytology ; growth & development ; Male ; Mitochondrial Proton-Translocating ATPases ; metabolism ; Neurons ; drug effects ; metabolism ; Pregnancy ; Rats ; Rats, Wistar

Objective To evaluate the home-made occluder in the treatment of VSD and its mid-term and long-term results. Methods From Jan. 2004 to May 2007, percutaneous VSD closure therapy under X-ray monitoring was performed in 78 consecutive VSDpatients, including 43 males and 35 females with an average age of (16.5±8.6) years (ranged 3-37 years). TTE, ECG and Holter examinations were performed in 1 week, 3 months, 1 year and 2 years after the procedure. Results Seventy-three home-made occluder devices with a diameter of 5-16 mm (10.3±3.2 mm) were implanted successfully, with a technical successful rate of 93.6% (73/78). The whole course follow-up were carried out in all 73 successful cases (100%). One week after the procedure ITE detected residual shunt in 8 cases, and three months later the residual shunt was observed in 5 cases. Follow-up check at one and two years after the treatment the residual shunt disappeared completely. For the observation of arrhythmia, occasional atrial premature beats or ventricular premature beats occurred in 28 cases (38.4%) within one week after the procedure, and two patients developed grade Ⅲ complete atrioventricular block at the third day after the surgery. The arrhythmia disappeared after medication of prednisone and nutrient drugs for 4-10 days. Bundle branch blocks were observed in 8 cases (10.1%) at one-year and two-year follow-up. Conclusion For the treatment of VSD, the home-made Amplatzer occluder device is reliable and effective, although a close foUow-up is required after the surgery.

Objective To study the expression and clinical significance of thyroid transcription factor-1 (TTF-1) in stage Ⅰ non-small cell lung cancer (NSCLC) after operation and to 1 investigate the values in identification of the prognosis of stage Ⅰ NSCLC.Methods The expression of TTF-1 in 129 specimens of stage Ⅰ NSCLC was detected by immunohistochemistry.Results The positive rate of TTF-1 in stage Ⅰ NSCLC was 64.3％.There were significant differences in TTF-1 expression rate among pathological subtypes (x2 =25.231,P ＜ 0.001),different ages (x2 =4.581,P =0.032),sex (x2 =4.900,P =0.027) and differentiation degrees(x2 =11.519,P =0.019).Univariate analysis suggested that in the stage Ⅰ NSCLC patients,the median disease-free survival and overall survival of those positive for TTF-1 expression were superior to those negative:38.9 months versus 27.8 months (P =0.023) and 64.10 months versus 50.68months (P =0.013).Cox regression analysis suggested that TTF-1 expression,tumor differentiation degrees were independent factors affecting the prognosis of stage Ⅰ NSCLC.Conclusion Patients with TTF-1 positive expression often had better survival.Positive expression of TTF-1 and differentiation degree of tumor were independent prognostic factors to affect the prognosis of NSCLC.

OBJECTIVE: To explore the therapeutic methods and effect of pingyangmycin injection in huge hemangioma at Hypopharynx and laryngeal under suspension laryngoscopy. METHOD: To ananysize the medical records of 12 Patients with giant hemangioma at hypopharynx and laryngeal. Patients were underwent polysomnography before operation. Inseted the suspension laryngoscopy to pars laryngea by mouth to exposing the hemangioma under general anaesthesia, then injected Pingyangmycin by a long-tube needling (one time per three weeks, accumulated dose less than 50 mg). RESULT: Among all patients, ten were cured and two showed obvious improvement. The data of PSG after operation indicates that the symptom of anoxia of post-operation were significantly ameliorated than that of pre-operation, no recurrence occurred during one year follow-up of the hemangioma. CONCLUSION The methods of pingyangmycin injection under suspension laryngoscopic surgery is significantly enhanced clinical effect and improved the life quality of patients with huge hemangioma, which with low risk, high security, can ameliorating anoxia, complete cure, and is a ideal therapeutic measure.
Adult ; Bleomycin ; administration & dosage ; analogs & derivatives ; Female ; Hemangioma ; therapy ; Humans ; Hypopharynx ; pathology ; Laryngoscopy ; Larynx ; pathology ; Male ; Middle Aged ; Treatment Outcome ; Young Adult