This study was aimed to explore the compatible mechanism of Shanyao, Fuling and Baishu in traditional Chinese medicine (TCM) cosmetology. Based on compatible principles, combination characteristics and modern relat-ed researches, the authors made an in-depth analysis on the modern scientific meaning and clinical application of Shanyao, Fuling and Baishu. The results showed that the compatibility of Shanyao, Fuling and Baishu was mainly used as a compatible group in TCM cosmetology. It was concluded that the compatible mechanism of Shanyao, Fuling and Baishu was flexible, which can be widely used in the clinical practice.

This study was aimed to analyze the influence of Dioscorea opposita Thunb. by processing methods from the aspect of formula. The search was made on formulae containing Dioscorea opposita Thunb. from the classic book For-mula Dictionary of Traditional Chinese Medicine. Processing methods were analyzed and shown with charts by EXCEL. The results showed that the most frequently used processing methods for Dioscorea opposita Thunb. were stir-heating, dry and crude in the clinical practice. Among them, the stir-heating Dioscorea opposita Thunb. was good at reinforcing the spleen and the kidneys. The crude produce was good at tonifying the lungs. And the dry product was good at rein-forcing the kidneys. It was concluded that the processing methods had obvious influence on the clinical function of Dioscorea opposita Thunb., which can be used as reference of more value for the clinics and health cultivation.

Medicine, Chinese Traditional ; Reproductive Techniques, Assisted

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; Reproductive Techniques, Assisted

Objective To explore the pattern and quantify the heat shock protein HSP)70 and HSP70 mRNA in Vero-E6 cells after infection with Hantann virus(HTNV).Methods The expres- sion of HSP70 and change of its mRNA level were detected by immunocytochemical staining,nucleic acid hybridization in situ and RT-PCR.Results In situ hybridization and RT-PCR were used to eval- uate the level of HSP70 mRNA during Hantaan 76-118 infection.HSP70 mRNA increased 0.5 h after infection,reached its peak by 12 h and gradually declined to steady state level by 72 h(vs.sham infec- ted group,P＜0.05).The expression of HSP70 protein induced by Hantaan 76-118 infection was e- valuated by quantitative immunocytochemical staining.HSP70 increased 0.5 h after infection,reached its peak by 12 h and decreased at 72 h after infection(vs.sham infected group,P＜0.05).Conclu- sions HSP70 can be induced directly by HTNV infection at both mRNA and protein levels,It pro- vides a basis for the further study of the pathogenesis,prevention and treatment of hemorrhagic fever with renal syndrome(HFRS).

Objective:To study heat shock proteins(HSP) and their association with Hantavirus nucleocapsid protein(HV-NP) in Vero-E6 cells infected with Hantaan76-118.Methods: The HV-NP was identified by immunocytochemical staining after infected with Hantaan76-118.The expression of HSP was detected by Western-blot and analyzed by double specific antibody sandwich ELISA.Results: Western-blot exhibited that the expressions of HSP27,HSP70 and Grp94 in the Vero-E6 cells infected with Hantaan 76-118 were significantly higher than those in the control cells(P

Purpose:To discuss which is the better mode of operation for esophageal carcinoma after radiation therapy. Methods:We divided 232 esophageal carcinoma patients into two groups (group A 116 patients and group B 116 patients) random. Operation mode of group A was posterolateral thoracotomy through the bed of sixth rib in right thorax, retrosternal replacement esophagus with stomach and left neck anastomosis or esophagus exteriorization. Correspondingly operation mode of group B was anterolateral thoracotomy through the intercostal space of fourth rib in right thorax, esophageal pouch replacement esophagus with stomach and left neck anastomosis or esophagus exteriorization. We analyzed the resection rate, the mortality rate, the duration of operation, the volume of bleeding and total incidence of complication in each group. Results:The resection rate was 100% in group A and 92.24% in group B ( P 0.05). Conclusions:Operation mode of group A is a better mode of operation for esophageal carcinoma after radiation therapy which is based on the resection rate, which is higher in group A than in group B and total incidence of complication which is lower in group A than in group B.

Background:Ulcerative colitis(UC)is a chronic and nonspecific intestinal inflammatory disease and its pathogenic mechanism has not yet been clarified. Intestinal mucosal immune function disorder may play a key role in the pathogenesis of UC. Aims:To investigate the effects of Wumeiwan on expressions of δ-opioid receptor(DOR),β-arrestin1 and Bcl-2 in rats with colitis. Methods:Fifty-six Sprague-Dawley rats were randomly divided into model group,Wumeiwan group, mesalazine group and blank group. Rats in model group,Wumeiwan group and mesalazine group were administered intrarectally with 5% TNBS and 50% ethanol to induce experimental colitis. After colitis models were established,rats in Wumeiwan group and mesalazine group were administered intragastrically with Wumeiwan and mesalazine suspension, respectively,and rats in model group and blank group were given intragastrically with 0. 9% NaCl solution,all for 15 days. On day 16,all the rats were sacrificed and colon samples were obtained. Protein and mRNA expressions of DOR,β-arrestin1 and Bcl-2 in colonic tissue were determined by immunohistochemistry and real-time PCR,respectively. Results:The inflammatory injury in colonic tissue of rats with experimental colitis was significantly attenuated when treated with Wumeiwan,Protein and mRNA expressions of DOR,β-arrestin1 and Bcl-2 in colonic tissue of model group were significantly higher than those of blank group(P < 0. 05). Protein and mRNA expressions of DOR,β-arrestin1 and Bcl-2 in colonic tissue of Wumeiwan group and mesalazine group were significantly lower than those of model group(P < 0. 05), however,no significant differences were found between the two groups(P > 0. 05). Conclusions:DOR-β-arrestin1-Bcl-2 signal transduction pathway may play a central role in the pathogenesis of UC. Intervening this signaling pathway may be one of the mechanisms of attenuating UC by Wumeiwan.

AIM: To study the inhibitive effects of valsartan and spironolactone on expression of insulin like growth factor 1 in cardiacmyocytes of spontaneously hypertensive rats. METHODS: 18 SHRs were randomly divided into 3 groups (n=6 in each): valsartan group treated with 30 mg?kg -1 ?d -1 valsartan, spironolactone group treated with 20 mg?kg -1 ?d -1 spironolactone, and control group treated with placebo. All were administrated by gastric perfusion. 6 WKY rats were served as control. After 13 weeks administration, the expressions of IGF 1 in cardiacmycytes were measured by immunohistochemistry and the IGF 1 concentrations in myocardium were measured by radiommunoassay. RESULTS: The expression of IGF 1 was higher in SHR group than that in the WKY group (P

Objective To investigate the relationship between thyroid hormone and uric acid (UA) and body mass index (BMI) in patients with type Ⅱ diabetes and normal thyroid function.Methods Total of 313 patients with type Ⅱ diabetes and normal thyroid function were selected.BMI,fasting blood glucose (FBG),the metabolism of blood lipid,thyroid hormones and UA indicators were examined and the correlations of thyroid hormone,BMI and UA were analyzed.Results (1) The patients were divided into two groups according to gender,and FT3,FT4,and UA of male were found to be significantly higher than those in female (P＜0.01).TSH,SBP,HDL-C in female were significantly higher than those in male (P＜0.01);(2) The patients were divided into three groups according to BMI Level.Thyroid-stimulating hormone (TSH),three iodine armour gland original glycine (TT3),free three iodine thyroid glycine (FT3),UA,and FBG in overweight and obesity groups were found to be higher than those in normal weight group (P ＜ 0.05);(3) The patients were divided into two groups according to the TSH level.Serum uric acid,TT3,FT3,fasting insulin in the group with TSH above 2.5 uIU/L were found to be higher than those in the group with TSH under 2.5 uIU/L (P ＜ 0.05);(4) Patients were divided into two groups according to the UA level.TSH,FT3 in male with high uric acid were found to be higher than those in male with normal uric acid (P ＜ 0.05);TSH was in female with high uric acid was found to be higher than that in female with normal uric acid (P ＜ 0.05).Conclusion Thyroid hormone in patients with type Ⅱ diabetes can be used to assess the body weight and uric acid,which is of great clinical importance.