Objective:To investigate the anti-myeloma cells specific immune activity induced by myeloma protein-pusled dendritic cells(DCs).Methods: DCs were obtained from peripheral blood(PB) of multiple myeloma(MM) patients after co-culture with GM-CSF,IL-4 and TNF-?.The phenotype of DCs was examined by flow cytometry.Host lymphocytes were stimulated with DCs pulsed with myeloma protein acquired from serum of MM patients on DEAE-celluose;lymphocytes treated with unpulsed-DC served as control.[~(3)H]-thymidine incorporation and 4 h ~(51)Cr-release assay were used to detect the proliferation and cytotoxicity against different targets(auto-myeloma cells,U266 cells) by T lymphocytes.Results: In vitro proliferation and cytotoxicity of T lymphocytes activated by myeloma protein pulsed-DCs were greater than those of T lymphocytes in the control group(P