As the new health care reform have been gradually deepened, hospital internal audit staffs should change ideas, change work strategies and methods, expand the area of audit business. Combining the working practices and experiences of the internal auditing, discuss the area of hospital internal auditing business under the new situation, then put forward suggestions for the internal audit function.

Small cell lung cancer (SCLC) are divided into limited and extensive stage diseases. Limited-stage SCLC (LS-SCLC) is conifned to the ipsilateral hemithorax and within a single radiotherapy region, 30%-40%of patients with SCLC are limited stage. The median ranges of survival for patients with LS-SCLC is from 15 to 20 months. Approximately 20%-40%of patients with LS-SCLC can survive for two years. According to the guidelines, patients with LS-SCLC should be treated with combined concurrent chemoradiotherapy and patients with LS-SCLC achieving a complete remission or patient with stage I disease who have had resection should be offered prophylactic cranial irradiation (PCI). Whereas in SCLC, targeted therapies is still in the early stage and few clinical trial data is available to support its effect.

Objective To determine the relationship between serum vitamin B_(12)(VB_(12))level and multiple sclerosis(MS).Methods The pertinent articles of the VB12 and the case control studies of MS were retrieved comprehensively by manual retrieval and computer retrieval.The methodology quality of the retrieved artilces were evaluated,and the arcb'cles were screened.Heterogeneity test was performed.The valid data was extracted and analyzed by Stata 10.1.Results In this study,9 studies were included.A total of 807 subjects were enrolled,including 414 patients and 393 controls.The Meta-analysis showed that the serum level of VB_(12) in patients with MS was lower than that in controls(standardized mean difference-0.24,95% CI-0.39 to-0.10).The sensitivity analysis indicated that the result of meta-analysis was reliable.Conclusion The serum level of VB_(12) might be associated with MS,and the deficiency of VB_(12) may contribute to the development of MS.

OBJECTIVE: To study the effect of a novel naphthalimide-polyamine conjugate on apoptosis of human hepatocellular carcinoma HepG2 cell line. METHODS: MTT assay was used to evaluate the cell inhibition rate. The cellular morphous was detected with AO/EB/Hoechst staining, the ROS was detected with DCFH-DA staining, the mitochondrial membrane potential (ΔΨm) was detected with Rh123 staining, the expression of caspase-9 and PARP-1 was detected by immunofluorescence method. The cell cycle was detected by HCS. Apoptosis of HepG2 cells was quantified by flow cytometry using Annexin V/PI stain. RESULTS: Proliferation of HepG2 cells was inhibited significantly by the naphthalimide-polyamine conjugate in a dosage dependant manner. Under HCS, some HepG2 cells underwent a typical apoptotic morphologic change and the expression of ROS and caspase-9 was increased. The expression of PARP-1 and the mitochondrial membrane potential (ΔΨm) was detected decreased. Flow cytometry indicates 64.12% HepG2 cells were induced apoptosis after 48 h incubation with 25 μmol·L-1 naphthalimide-polyamine conjugate. CONCLUSION: The naphthalimide-polyamine conjugate could significantly induce the apoptosis of HepG2 cells by the mitochondrial pathway. The mechanism is concerned with increasing ROS, decreasing the mitochondrial membrane potential, upgrading caspase-9 and decreasing the expression of PARP-1.

OBJECTIVE: To explore the masking efficiency and regularity of bitterness suppressants (BS) to a representative bitterness substance berberine hydrochloride (BBR). METHODS: Some quantitative evaluation methods of BS efficiency were established based on relevant literature and our previous research. Efficiency of respectively added into eight kinds of BS such as acesulfame, on bitter degree 3 BBR solution (0.05 mg•mL-1) were evaluated using the established methods. Bitterness masking regularity of BS on BBR solution were explained by respectively establishing the relationships between tongue tasted bitter decreased value (ΔI), electronic tongue tasted bitter decreased value (ΔIe) with BS concentration (ρ). RESULTS: ①Five tongue taste bitter masking efficiency (calibrated masking rate, bitterness revised standard half effect index and so on), two electronic tongue bitter masking efficiency(first taste reduce and aftertaste reduce) were established. ②Seven kinds of bitterness suppressants efficacy indicators can be used to compare the bitterness suppressants efficacy of BS to BBR solution, and the maximum bitterness reduction of 8 kinds of BS to BBR solution is above 1.8. ③ΔI-ρ Weibull regularity models of eight BS and nine ΔIe-ρ models of six BS were brlilt.HP-β-CD, glycine and crystalline fructose had good response to two sensor, stevioside, soluble soybean polysaccharide and acesulfame had a good response to one sensor and xanthan gum and aspartame had no response to any sensor. CONCLUSION: Seven evaluation indexes and methods are built based on human tongue and electronic tongue taste method. Max standard capability index of BS is suitable for the development of bitterness suppressants in taste method and two indicators of electronic tongue are also can be used as indicative indicators. Masking regularity of BS to BBR solution is exposed which could provide reference for the development and application of more BS.

The establishment of fingerprint system and the study of spectrum-effect relationship have become a very important development trend in quality evaluation of Chinese materia medica (CMM). It is based on the research of index components and can be used to evaluate the authenticity, quality and stability of crude drug, pieces and patent medicines of CMM. However, it is affected by many unstable factors and lacks of relevance to traditional Chinese medicine theories. Quality marker, in due course, provides an enlightening thought and way for the fingerprint system and spectrum-effect relationship studies. Taking Lonicerae Japonicae Flos as an example, this paper explores the researching ideas and establishes methods for fingerprinting research on CMM based on the characteristics of Q-markers.

ArcScan Insight 100 very high-frequency(VHF)digital ultrasound scanner is a new ocular ultrasonic measuring instrument, which can detect and measure the anterior segment. It can be used for screening before corneal refractive surgery and follow-up after corneal refractive surgery, measuring anterior segment parameters before implantable collamer lens(ICL)implantation, predicting preoperative vault, measuring postoperative vault, early screening keratoconus, and diagnosing glaucoma, cataract and eye injuries, etc. Taking the advantages of a wide range examination of ultrasound biomicroscope(UBM)and simple operation of optical coherence tomography(OCT), it has a broad prospect for clinical application. In this paper, the measurement principle, application method, parameters and clinical application progress of ArcScan Insight 100 VHF digital ultrasound scanner are reviewed in detail.

OsRhoGDI2 was isolated as a putative partner of Rho protein family member OsRacD from rice panicles by yeast two-hybrid, but its function remains unknown. In order to identify the function of OsRhoGDI2, OsRhoGDI2 knockout mutants were created by CRISPR/Cas9 technology. The results showed that two different homozygous mutants were obtained in T0 generation, and eight kinds homozygous mutants were identified in T1 generation. Sequence analysis revealed that the base substitution or base deletion occurred near the editing targets of the gene in knockout rice, and it could be expected that the truncated OsRhoGDI2 proteins lacking the RhoGDI conserved domain would be generated. Phenotype analysis showed that the OsRhoGDI2 knockout rice plants were significantly lower than the control plants. Statistical analysis confirmed that the significant decrease of plant height was due to the shortening of the second and third internodes, suggesting that OsRhoGDI2 gene may be related with rice height control.
CRISPR-Cas Systems ; Genes, Plant ; genetics ; Oryza ; genetics ; growth & development ; Plants, Genetically Modified ; rho Guanine Nucleotide Dissociation Inhibitor beta ; genetics

Objective To investigate the predictive value of serum fibroblast growth factor 23 (FGF23) and S100A12 for atherosclerosis cardiovascular disease (ASCVD) in elderly patients with diabetes. Methods A total of 133 elderly patients with diabetes were selected as the study subjects and divided into diabetes group (n=59) and ASCVD group (n=74) according to whether they had ASCVD or not. Healthy subjects who underwent physical examination at the same time were selected as the control group (n=56). The expression levels of serum FGF23 and S100A12 were detected and compared. Multivariate Logistic regression analysis was used to analyze the influencing factors of ASCVD in elderly diabetic patients. The predictive value of serum FGF23 and S100A12 levels for the occurrence of ASCVD was evaluated. Results The proportion of hypertension, fasting blood glucose and diabetes course in the ASCVD group were significantly higher or longer than those in the diabetes group (P < 0.05). The expression levels of serum FGF23 and S100A12 in the ASCVD group were significantly higher than those in the diabetes group and control group (P < 0.05). The expression levels of hypertension, serum FGF23 and S100A12 were the independent influencing factors for ASCVD in elderly patients with diabetes (P < 0.05). The area under the curve (AUC) of serum FGF23 and S100A12 to predict ASCVD in elderly diabetic patients were 0.755 and 0.874, respectively, and the AUC of serum FGF23 and S100A12 combined to predict ASCVD was 0.934, and the sensitivity and specificity were 82.43% and 89.83%, respectively. The combined prediction of serum FGF23 and S100A12 was better than the single prediction (P < 0.05). Conclusion Serum levels of FGF23 and S100A12 are increased in elderly patients with diabetes. The combination of serum FGF23 and S100A12 has a higher value in predicting ASCVD in elderly diabetic patients compared to their lonely detection.

Objective To investigate the diagnostic value of computer tomography (CT) combined with serum secreted frizzled-related protein 4 (SFRP4) and soluble suppression of tumorigenicity 2 (sST2) for pancreatitis after biliary duct stent implantation in patients with malignant biliary obstruction (MBO). Methods A total of 243 MBO patients with biliary duct stent implantation were selected as research objects, and they were divided into pancreatitis group with 105 cases and no pancreatitis group with 138 cases according to condition of postoperative complicated pancreatitis. Enzyme-linked immunosorbent assay was used to detect serum levels of SFRP4 and sST2, and the receiver operating characteristic (ROC) curve and the four table method were used to analyze the diagnostic values of serum SFRP4 or sST2 alone and their combination with CT for postoperative complicated pancreatitis. Results The serum levels of SFRP4 and sST2 in the pancreatitis group were significantly higher than those in the no pancreatitis group (P < 0.05); the values of area under the curve (AUC) of serum SFRP4 and sST2 levels for diagnosis of postoperative complicated pancreatitis were 0.694 and 0.667, respectively. The sensitivity and specificity of CT for diagnosis of postoperative complicated pancreatitis were 76.19% and 60.87%, respectively; the sensitivity and accuracy of CT combined with serum SFRP4 and sST2 levels in diagnosing postoperative complicated pancreatitis were 98.10% and 76.54%, respectively; the sensitivity and accuracy of the combination of the three indexes for diagnosis were significantly higher than those of CT, SFRP4 or sST2 alone (P < 0.05). Conclusion MBO patients with pancreatitis after biliary duct stent implantation have higher serum levels of SFRP4 and sST2, and CT combined with serum levels of SFRP4 and sST2 has high diagnostic value for postoperative complicated pancreatitis.