Background: Hemoglobin is a composition of erythrocyte. They are released from the lysis of erythrocyte. Objectives: to study free hemoglobin concentrations in surgical patients received massive blood transfusion (MBT). Subject and methods: 48 adult patients (male: 31 (64.6%), female: 17 (35.4%), mean age: 36 \xb1 16) with more than 3000ml of storage blood were tested for plasma free hemoglobin. The major reason for MBT were severe trauma (45.8%), the remainder were non - trauma. The change of Hb free was followed in 16 patients at the times of 1500 ml, 2000 ml, 3000 ml storage blood transfused (9 \xb1 3 days). Results: Plasma free hemoglobin was detected after 2,000ml of red cell transfusion and during the first 24h post MBT in 100% cases. Plasma free hemoglobin concentration trended to increase with quantity of transfused blood (0.56 \xb1 0.3 g/dl after 2000 ml, 0.76 \xb1 0.47 g/dl after 3000 ml, and 0.93 \xb1 0.41 g/dl after 5000 ml). There was no correlation between free hemoglobin concentration in plasma of patients with the storage time of blood transfused, and no difference of plasma free hemoglobin concentration between in trauma and non trauma patients. Conclusion: The blood with short time of storage is recommended after 1500ml blood transfusion in these patients. \r\n', u'\r\n', u'
Blood Transfusion ; Hemoglobins/ pharmacokinetics