No abstract available.
Blood Glucose* ; Hemoglobin A, Glycosylated*

BACKGROUND: We evaluated the performance and analysis time of HLC-723 G7 (Tosoh corp. Tokyo, Japan) hemoglobin (Hb) A1c autoanalyzer. It utilizes cation exchange high performance liquid chromatography (HPLC) method and has a reduced analysis time compared with that of an earlier model HLC-723GHb V A1c 2.2(TM) (HLC-723GHb V, Tosoh corp. Tokyo, Japan). METHODS: We evaluated linearity, precision and comparison with HLC-723GHb V following NCCLS guidelines and counted the number of tests per hour to estimate analysis time. RESULTS: Linearity through the range from 5.8% to 13.9% was good (r2=0.9930, relative nonlinearity <2.5%). The within-run coefficients of variation (CVs) for groups of low, middle, and high level were 1.09%, 0.76%, and 0.68% and total CVs for each group were 1.60%, 0.91%, and 1.00%, respectively. Correlation equation between HLC-723 G7 and HLC-723GHb V was HLC-723 G7=1.0308 (HLC-723GHb V)-0.2896 %Hb A1c (r=0.9992, P<0.0001). Analysis time of HLC-723 G7 was 1.2 minutes per test compared with 2.1 minutes of HLC-723GHb V. CONCLUSIONS: HLC-723 G7 showed the acceptable performance and shortening analysis time therefore, it was suitable for reducing turn around time of Hb A1c assay.
Chromatography, Liquid ; Hemoglobin A, Glycosylated

No abstract available.
Hemoglobin A, Glycosylated* ; Humans

No abstract available.
Hemoglobin A, Glycosylated* ; Humans

The relationship between the relative amounts of HbH and the proportions of RBC with inclusion bodies (IBs) as shown that almost straight, getting a correlation coefficient of 0.86, the regression line cuts the x- axis of RBC with lbs at 19%, indicating that demonstration of RBC with IBs is more sensitive in detecting HbH than electrophoresis. Strickingly the mean level of both HbH and percentage of RBC with lbs were significantly higher in a- than 1/HbCS than in a- than 1/ a -than2. These results led us to conclude that examination of the intraerythrocytic inclusion bodies is able to be used as a “probe” technique in unequiped laboratory for detecting HbH disease.
Erythrocytes ; Hemoglobin H

No abstract available.
Adult ; Fasting ; Glucose ; Hemoglobin A, Glycosylated ; Hemoglobin, Sickle ; Humans ; Plasma

No abstract available.
Adult ; Fasting ; Glucose ; Hemoglobin A, Glycosylated ; Hemoglobin, Sickle ; Humans ; Plasma

No abstract available.
Hemoglobin A* ; Humans ; Mothers* ; Postpartum Period*

No abstract available.
Cystatin C* ; Hemoglobin A, Glycosylated* ; Humans

High performance liquid chromatography (HPLC) is currently the mainstream technology for detecting hemoglobin. Glycated hemoglobin (HbA1c) is a gold indicator for diagnosing diabetes, however, the accuracy of HbA1c test is affected by thalassemia factor hemoglobin F (HbF)/hemoglobin A2 (HbA2) and variant hemoglobin during HPLC analysis. In this study, a new anti-interference hemoglobin analysis system of HPLC is proposed. In this system, the high-pressure three-gradient elution method was improved, and the particle size and sieve plate aperture in the high-pressure chromatography column and the structure of the double-plunger reciprocating series high-pressure pump were optimized. The system could diagnose both HbA1c and thalassemia factor HbF/HbA2 and variant hemoglobin, and the performance of the system was anti-interference and stable. It is expected to achieve industrialization. In this study, the HbA1c and thalassemia factor HbF/HbA2 detection performance was compared between this system and the world's first-line brand products such as Tosoh G8, Bio-Rad Ⅶ and D10 glycosylated hemoglobin analysis system. The results showed that the linear correlation between this system and the world-class system was good. The system is the first domestic hemoglobin analysis system by HPLC for screening of HbA1c and thalassemia factor HbF/HbA2 rapidly and accurately.
Chromatography, High Pressure Liquid ; Fetal Hemoglobin/analysis* ; Glycated Hemoglobin A/analysis* ; Hemoglobin A2/analysis* ; Hemoglobins