To investigate the reason of extraction, length, number and transverse morphology of the root and root canals of first permanent mandibular molars. 100 extracted first permanent mandibular molars were collected. The length of mesial and distal root of the first permanent mandibular molars was 13.00 mm and 13.51 mm separately. 75% double roots and 25% three roots were observed under the stereomicroscope. Difference of root number and shape of transverse morphology in mesial root were more obvious than those in distal roots of the same first mandibular molars in this study.

To investigate the reason of extraction,length,number and transverse morphology of the root and root canals of first permanent mandibular molars.100 extracted first permanent mandibular molars were collected.The length of mesial and distal root of the first permanent mandibular molars was 13.00 mm and 13.51 mm separately.75% double roots and 25% three roots were observed under the stereomicroscope.Difference of root number and shape of transverse morphology in mesial root were more obvious than those in distal roots of the same first mandibular molars in this study.

objective To study the inhibition effect and its mechanism of a non - cytotoxic chemotherapy drug in the growth of gastric cancer. Methods The inhibition effect was observed in the growth of xenotransplanted gastric cancer cells MKN45 with a gastrin receptor(GR) antagonist. On the basis of the determination of expressed GR in 34 patients with gastric cancer, we evaluated the preliminary clinical effect of PGL to gastric cancer with the positive GR. Results It is less tumor area, weight and gastric cancer cells DNA Index, average DNA contents and S phase fraction in PGL group than in control group. It is the least in PGL + SST group. GR is easy to be expressed in advanced gastric cancer of the body, cardia and fundus. With the 16-21 months follow- up of 14 patients with positive GR gastric cancer, we find that 2 patients died in control group of 8 patients, and all patients are alive in PGL group. Conclusion PGL can regulate the growth of gastric cancer cells MKN45. It would be able to improve the prognosis of gastric cancer.

0.05.3 year survival rate was 36.67%(A) and 57.14%(B),P

BACKGROUND: Osteoporosis is easily seen in postmenopausal women. The bone loss situation is different among populations of different age and body mass.OBJECTIVE: To analyze the effects of age, menopausal duration,menopause age, height, body mass and body mass index on bone mineral density (BMD) in postmenopausal women.DESIGN: Random sampling test was conducted to postmenopausal women.SETTING: A clinical trial center of a provincial Chinese traditional medicine research institute, an orthopaedic department of a university hospital and a bone wound department of a university.PARTICIPANTS: Totally 603 naturally postmenopausal women in Fuzhou area from September 2000 to August 2003.METHODS: Random sampling method was used. The age of participants,menopausal duration, menopause age, height, body mass and bone mineral index(BMI) were recorded. Dual energy X-ray bone mineral density machine was used to examine the bone mineral density of lumbar, neck of femur, great trochanter and Ward' s area while SPSS software was used to conduct regression analysis.MAIN OUTCOME MEASURES: The correlation between age,menopausal years, menopause age, height, body mass, BMI and bone density as well as the regression equation.RESULTS: There was very significant correlation between age, menopausal duration, body mass, BMI of postmenopausal women and bone mineral density of lumbar, neck of femur, great trochanter and Ward' s area. The BMD in low body mass group is greatly lower than that of obesity group( P ＜ 0.01 ). The main factors impacting BMD of lumbar are in turn age, body mass and menopause age while the regression equation: y = 0. 927 - 0. 0093 X1+ 0. 0037X2 + 0. 004X3. The factors impacting BMD of neck of femur are in turn menopausal duration, body mass, menopause age and the regression equation was: y = 0. 687 - 0. 0081 X1 + 0. 0048 X2 - 0. 0034 X3. And the factors affecting BMD of greattrochanter are age, body mass and menopause age while the regression equation was y = 0. 591 - 0. 0038 X1 + 0. 0042 X2 - 0. 0024 X3. Age, body mass index and menopausal duration are main factors affecting BMD of Ward' s area and the regression equation was y =0. 686 -0. 0072 X1 +0. 0136 X2 -0. 0046 X3.CONCLUSION: The BMD of lumbar and hip gradually decreases with the increase of age in postmenopausal women. The risk of getting osteoporosis in people with low body mass is greater than women in normal body mass groupand obesity group.

Objective To investigate the effects of zinc fingers and homeoboxes 3 (ZHX3) silence on expressions of smad3, smad4 and RUNX2 in bone marrow mesenchymal stem cells (BMSCs). Methods ZHX3 low expression vector (ZHX3 silent group) was constructed and was transfected to rat BMSCs. Empty vector was transfected into BMSCs and was used as vehicle control group, and wild type BMSCs was used as the control group. The cell transfection rate was measured under a fluorescence microscope, and then the successful transfection was identified. The immunocytochemistry and immu?noblotting methods were used to detect the expression levels of smad3, smad4 and RUNX2. Results (1) Cells with BMSCs phenotype can be obtained by recovery culturing. (2) After transfection, the green fluorescent protein was found in ZHX3 si?lence group and vehicle control group. Blank control group showed no significant fluorescence. The expression level of ZHX 3 was significantly lower in ZHX3 silence group than that of vehicle control group. (3) Results of immunofluorescence asssay showed that the positive expressions of smad3 and smad4 were located in nucleus and cytoplasm, the positive expression of RUNX2 was mainly located in nucleus. Positive cells were observed in three groups. There was no significant difference in fluorescence intensity between the control group and the vehicle control group, but the fluorescence intensity was significant?ly lower in ZHX3 gene silence group than that of two control groups. (4) There were no significant differences in expressions of smad3, smad4 and RUNX2 betweem control group and the vehicle control group, but they were significantly higher than those of ZHX3 silence group(P < 0.05). Conclusion ZHX3 gene silence can delay vitro osteogenesis of BMSCs, which may play a role by the down-regulated expression levels of smad3, smad4 and RUNX2.

Objective To compare different ways to trace bone marrow mesenchymal stem cells (BMSCs) after being transplanted in cerebral ischemia-reperfusion injury. Methods Male SD rats of SPF grade were randomly divided into sham group, model group (ischemia-reperfusion,IR), BrdU tracing group, PKH26 tracing group and GFP tracing group. Fo?cal cerebral ischemia-reperfusion model was established by blocking middle cerebral artery. 24 hours after cerebral isch?emia-reperfusion injury, 10μL BMSCs that were labeled respectively by BrdU, PKH26, GFP were added respectively into BrdU, PKH26 and GFP tracing group while equal volum of normal saline was added into sham group and model group. Mod?el and transplanting cells efficacy was determined by neural behavioral score, TTC staining and brain water content;Neurons were counted using tar violet staining;The number of transplant cells in the transplanting site was assessed by fluorescence microscopy. Results Before transplanting, there was no significant difference among BrdU, PKH26 and GFP group in cell labeled efficacy. By contrast, neural behavioral score, brain infarct volume and brain tissue water content were significantly lower in all three tracing groups than that in model group 4 weeks after transplantation while neuron counts were markedly higher. There was no significant difference of above parameters among the three tracing groups. However, the number of traced transplanting cells in damaging area in GFP group is significantly higher than that in BrdU group and PKH26 group. Conclusion In cerebral ischemia-reperfusion injury, the tracing effect of GFP last longer, therefore it is significantly more effective than BrdU and PKH26.

Objective To investigate the relationship between ovary excision and serum levels of TNF ?, TGF ? 1. Methods Serum levels of TNF ? and TGF ? 1 in ovariectomized rats were measured by ELISA. Results The serum levels of TNF ? were increased〔(53 88?16 35)ng/L〕, but the TGF ? 1 were decreased 〔(6 78?0 75)?g/L〕 in ovariectomized rats when E 2 decreased compared with those of the Sham group( P

Objective:To establish a method by combining microwave-assisted extraction(MAE),solid phase extraction(SPE)and high performance liquid chromatography(HPLC)for determination of the mefenacet residues in rice.Methods:Acetone and acetontrile(37)were used as extraction solvent.Microwave-assisted extraction was used to extract mefenacet residues in the rice.The extracts were then cleaned up with a Florisil cartridge and then subjected to Hypersil C18 column(5 ?m,4.6 mm?200 mm),with acetonitrilewater(5050,V/V)solution as mobile phase and with a flow rate of 1.0 ml/min;the ultraviolet detection wavelength was at 217 nm.Results:Good linear correlation for mefenacet was found within a concentration range of 0.198-9.900 ?g/ml.The detection limit was 0.039 6 ?g/mL for mefenacet(S/N=2).The average recovery rate of rice hull and brown rice were 90.8%(RSD 1.8%)and 85.6%(RSD 2.5%),respectively.Conclusion:The present method is simple and rapid;it can be used for the determination of mefenacet residues in rice.

Objective To build a digitized 3D model of the pulmonary venous system of Chinese people for the purpose of providing anatomic information of the pulmonary venous system for anatomic teaching,imaging diagnosis and surgical operation of pulmonary diseases. Methods The primal images from the first Chinese visible human data set of chest were converted into image sequence only cantaining the sections of pulmonary veins after manual registration and image segmentation.Then these images were imported into 3DMed software to build a 3D model of the pulmonary venous system through threshold registration algorithm. Results The three dimensional structures of the pulmonary venous system were reconstructed entirely.All reconstructed structures could be displayed individually or jointly,and could be rotated continuously on any plane.Conclusion The branches and the spacial characters of the pulmonary venous system can be clearly shown on the reconstructed 3D model.It will help anatomy teaching and provide morphological data for image diagnosis and lung operations.