To investigate the reason of extraction, length, number and transverse morphology of the root and root canals of first permanent mandibular molars. 100 extracted first permanent mandibular molars were collected. The length of mesial and distal root of the first permanent mandibular molars was 13.00 mm and 13.51 mm separately. 75% double roots and 25% three roots were observed under the stereomicroscope. Difference of root number and shape of transverse morphology in mesial root were more obvious than those in distal roots of the same first mandibular molars in this study.

To investigate the reason of extraction,length,number and transverse morphology of the root and root canals of first permanent mandibular molars.100 extracted first permanent mandibular molars were collected.The length of mesial and distal root of the first permanent mandibular molars was 13.00 mm and 13.51 mm separately.75% double roots and 25% three roots were observed under the stereomicroscope.Difference of root number and shape of transverse morphology in mesial root were more obvious than those in distal roots of the same first mandibular molars in this study.

objective To study the inhibition effect and its mechanism of a non - cytotoxic chemotherapy drug in the growth of gastric cancer. Methods The inhibition effect was observed in the growth of xenotransplanted gastric cancer cells MKN45 with a gastrin receptor(GR) antagonist. On the basis of the determination of expressed GR in 34 patients with gastric cancer, we evaluated the preliminary clinical effect of PGL to gastric cancer with the positive GR. Results It is less tumor area, weight and gastric cancer cells DNA Index, average DNA contents and S phase fraction in PGL group than in control group. It is the least in PGL + SST group. GR is easy to be expressed in advanced gastric cancer of the body, cardia and fundus. With the 16-21 months follow- up of 14 patients with positive GR gastric cancer, we find that 2 patients died in control group of 8 patients, and all patients are alive in PGL group. Conclusion PGL can regulate the growth of gastric cancer cells MKN45. It would be able to improve the prognosis of gastric cancer.

0.05.3 year survival rate was 36.67%(A) and 57.14%(B),P

Objective To investigate the relationship between ovary excision and serum levels of TNF ?, TGF ? 1. Methods Serum levels of TNF ? and TGF ? 1 in ovariectomized rats were measured by ELISA. Results The serum levels of TNF ? were increased〔(53 88?16 35)ng/L〕, but the TGF ? 1 were decreased 〔(6 78?0 75)?g/L〕 in ovariectomized rats when E 2 decreased compared with those of the Sham group( P

Objective To investigate the effects of zinc fingers and homeoboxes 3 (ZHX3) silence on expressions of smad3, smad4 and RUNX2 in bone marrow mesenchymal stem cells (BMSCs). Methods ZHX3 low expression vector (ZHX3 silent group) was constructed and was transfected to rat BMSCs. Empty vector was transfected into BMSCs and was used as vehicle control group, and wild type BMSCs was used as the control group. The cell transfection rate was measured under a fluorescence microscope, and then the successful transfection was identified. The immunocytochemistry and immu?noblotting methods were used to detect the expression levels of smad3, smad4 and RUNX2. Results (1) Cells with BMSCs phenotype can be obtained by recovery culturing. (2) After transfection, the green fluorescent protein was found in ZHX3 si?lence group and vehicle control group. Blank control group showed no significant fluorescence. The expression level of ZHX 3 was significantly lower in ZHX3 silence group than that of vehicle control group. (3) Results of immunofluorescence asssay showed that the positive expressions of smad3 and smad4 were located in nucleus and cytoplasm, the positive expression of RUNX2 was mainly located in nucleus. Positive cells were observed in three groups. There was no significant difference in fluorescence intensity between the control group and the vehicle control group, but the fluorescence intensity was significant?ly lower in ZHX3 gene silence group than that of two control groups. (4) There were no significant differences in expressions of smad3, smad4 and RUNX2 betweem control group and the vehicle control group, but they were significantly higher than those of ZHX3 silence group(P < 0.05). Conclusion ZHX3 gene silence can delay vitro osteogenesis of BMSCs, which may play a role by the down-regulated expression levels of smad3, smad4 and RUNX2.

Objective To compare different ways to trace bone marrow mesenchymal stem cells (BMSCs) after being transplanted in cerebral ischemia-reperfusion injury. Methods Male SD rats of SPF grade were randomly divided into sham group, model group (ischemia-reperfusion,IR), BrdU tracing group, PKH26 tracing group and GFP tracing group. Fo?cal cerebral ischemia-reperfusion model was established by blocking middle cerebral artery. 24 hours after cerebral isch?emia-reperfusion injury, 10μL BMSCs that were labeled respectively by BrdU, PKH26, GFP were added respectively into BrdU, PKH26 and GFP tracing group while equal volum of normal saline was added into sham group and model group. Mod?el and transplanting cells efficacy was determined by neural behavioral score, TTC staining and brain water content;Neurons were counted using tar violet staining;The number of transplant cells in the transplanting site was assessed by fluorescence microscopy. Results Before transplanting, there was no significant difference among BrdU, PKH26 and GFP group in cell labeled efficacy. By contrast, neural behavioral score, brain infarct volume and brain tissue water content were significantly lower in all three tracing groups than that in model group 4 weeks after transplantation while neuron counts were markedly higher. There was no significant difference of above parameters among the three tracing groups. However, the number of traced transplanting cells in damaging area in GFP group is significantly higher than that in BrdU group and PKH26 group. Conclusion In cerebral ischemia-reperfusion injury, the tracing effect of GFP last longer, therefore it is significantly more effective than BrdU and PKH26.

BACKGROUND: Osteoporosis is easily seen in postmenopausal women. The bone loss situation is different among populations of different age and body mass.OBJECTIVE: To analyze the effects of age, menopausal duration,menopause age, height, body mass and body mass index on bone mineral density (BMD) in postmenopausal women.DESIGN: Random sampling test was conducted to postmenopausal women.SETTING: A clinical trial center of a provincial Chinese traditional medicine research institute, an orthopaedic department of a university hospital and a bone wound department of a university.PARTICIPANTS: Totally 603 naturally postmenopausal women in Fuzhou area from September 2000 to August 2003.METHODS: Random sampling method was used. The age of participants,menopausal duration, menopause age, height, body mass and bone mineral index(BMI) were recorded. Dual energy X-ray bone mineral density machine was used to examine the bone mineral density of lumbar, neck of femur, great trochanter and Ward' s area while SPSS software was used to conduct regression analysis.MAIN OUTCOME MEASURES: The correlation between age,menopausal years, menopause age, height, body mass, BMI and bone density as well as the regression equation.RESULTS: There was very significant correlation between age, menopausal duration, body mass, BMI of postmenopausal women and bone mineral density of lumbar, neck of femur, great trochanter and Ward' s area. The BMD in low body mass group is greatly lower than that of obesity group( P ＜ 0.01 ). The main factors impacting BMD of lumbar are in turn age, body mass and menopause age while the regression equation: y = 0. 927 - 0. 0093 X1+ 0. 0037X2 + 0. 004X3. The factors impacting BMD of neck of femur are in turn menopausal duration, body mass, menopause age and the regression equation was: y = 0. 687 - 0. 0081 X1 + 0. 0048 X2 - 0. 0034 X3. And the factors affecting BMD of greattrochanter are age, body mass and menopause age while the regression equation was y = 0. 591 - 0. 0038 X1 + 0. 0042 X2 - 0. 0024 X3. Age, body mass index and menopausal duration are main factors affecting BMD of Ward' s area and the regression equation was y =0. 686 -0. 0072 X1 +0. 0136 X2 -0. 0046 X3.CONCLUSION: The BMD of lumbar and hip gradually decreases with the increase of age in postmenopausal women. The risk of getting osteoporosis in people with low body mass is greater than women in normal body mass groupand obesity group.

Objective To understand the fairness of health resources of endemic diseases institutions in Henan Province,to provide evidence for endemic allocation of health resources for the government.Methods Health-related data resources in endemic disease prevention and control institutions were collected in Henan Province,including personnel,business space area,the value of equipment,and health funding,etc.The percentages of population distribution and geographical distribution of health resources were calculated; Lorenz curves of demographic fairness and geographic fairness were drawn.According to Lorenz curve (Gini coefficients of the population and the geographical distribution were calculated based on Lorenz curve),Gini coefficients were used to evaluate the fairness of the endemic disease prevention institutions for health resource allocation.Results As can be seen from Lorenz curve,the population fairness of endemic disease prevention and health personnel was the best,but the number of senior officers was less and the total value of equipment owned was low.The geographical fairness of all the indicators of health resources was poorer,and geographic fairness among senior officers was the worst.The Gini coefficient of health technical personnel,senior personnel,undergraduate or above personnel,value of equipments,area of business room and health funding allocation determined by the population distribution was less than 0.4.The health resources were relatively fair and reasonable according to the distribution of population.The Gini coefficients of all the indicators determined by the distribution of geography were higher than those determined by the distribution of population.Furthermore,the geographic Gini coefficient of senior personnel was above 0.4 which was in the vigilance state.Conclusions The equality of health resources determined by population distribution in the institutions for endemic diseases control in Henan is superior to those determined by geographic distribution.The allocations of health resources of endemic diseases control in different areas are unbalanced.

Objective To summarize our experience in the prevention and treatment of right accessory hepatic duct and right hepatic duct injury during laparoscopic cholecystectomy. Methods The clinical data of 21 cases with right accessory hepatic duct or right hepatic duct during laparoscopic cholecystectomy were reviewed retrospectively. Result According to anatomy identified by preoperative work-up and selective cholangiography during the operation, 18 cases had the right accessory hepatic duct,eleven of them were confirmed intraoperatively. The accessory hepatic ducts were conserved in 3 cases and clipped without biliary leaks postoperativly in 7 cases; One case had biliary leaks postoperatively with the duct sutured intraoperatively, and recovered well conservative therapy. Accessory hepatic ducts were accidentally injuried in 7 cases, two patients were transferred to open surgery; three cases were confirmed to be injuried and clipped by second laparoscopic exploration because of biliary leaks postoperatively. Three cases had a low confluence of the right and left hepatic duct with the gallbladder duct joining the right bile duct, the ducts were conserved in 2 cases and injuried in one. Postoperatively all these 21 cases were followed up for 2 years, without jaundice or liver dysfunction. Conclusions To prevent injury of right accessory hepatic duct and right hepatic duct. High vigilance and familiarity with the anatomic variants of the biliary tree and intraoperative cholangiography in selective cases are fundmental.