Objective To explore the clinical effect of procaterol hydrochloride combined with Xiaokechuan capsule in the treatment of cough variant asthma(CVA)and its impact on serological indicators,airway function of children.Methods A total of 124 children with CVA admitted to the Zigong First People's Hospital from March 2019 to April 2022 were selected as the research subjects.The children were divided into control group and observation group according to random number table method,with 62 cases in each group.The children in the control group were treated with procaterol hydrochloride,and the children in the observation group were treated with procaterol hydrochloride and Xiaokechuan capsule for two weeks.The clinical efficacy of children was compared between the two groups after treatment.The cough scores during the day and night of children were evaluated in the two groups before and 2 weeks after treatment.The serum high mobility group protein B1(HMGB1),Toll like receptor 4(TLR4),nuclear factor-κB(NF-κB),interleukin-4(IL-4),interferon-γ(INF-γ)levels of children in the two groups were measured by enzyme linked immunosorbent assay before and 2 weeks after treatment,and the ratio of INF-γ/IL-was calculated.The 25％maximal expiratory flow-volume(MEF25),50％maximal expiratory flow-volume(MEF50),75％maximal expiratory flow-volume(MEF75)of children in the two groups were measured by lung function detector before and 2 weeks after treatment.The adverse reactions of children in the two groups were recorded during treatment.Results The total effective rate of children in the control group and observation group was 82.26％(51/62)and 95.16％(59/62),respectively;the total effective rate of children in the observation group was significantly higher than that in the control group(P＜0.05).There was no significant difference in cough scores during the day and night of children between the two groups before treatment(P＞0.05);the cough scores during the day and night of children after treatment were significantly lower than those before treatment in the two groups(P＜0.05);after treatment,the cough scores during the day and night of children in the observation group were significantly lower than those in the control group(P＜0.05).There was no significant difference in serum HMGB1,TLR4,NF-κB levels of children between the two groups before treatment(P＞0.05);the serum HMGB1,TLR4,NF-κB levels of children after treatment were significantly lower than those before treatment in the two groups(P＜0.05);after treatment,the serum levels of HMGB1,TLR4,and NF-κB of children in the observation group were significantly lower than those in the control group(P＜0.05).There was no significant difference in MEF25,MEF50,and MEF75 of children between the two groups before treatment(P＞0.05);the MEF25,MEF50,and MEF75 of children after treatment were significantly higher than those before treatment in the two groups(P＜0.05);after treatment,the MEF25,MEF50,and MEF75 of children in the observation group were significantly higher than those in the control group(P＜0.05).There was no significant difference in serum IL-4,INF-γ levels and the ratio of INF-γ/IL-4 of children between the two groups before treatment(P＞0.05);the serum IL-4 level of children after treatment were significantly lower than those before treatment,the INF-γ level and the ratio of INF-γ/IL-4 were significantly higher than those before treatment in the two groups(P＜0.05);after treatment,the serum IL-4 level of children in the observation group was significantly lower than that in the control group,the INF-γ level and the ratio of INF-γ/IL-4 were significantly higher than those in the control group(P＜0.05).All children had good drug tolerance during the treatment period,and no significant adverse drug reactions were observed.Conclusion The combination of Xiaokechuan capsules and procaterol hydrochloride has a significant therapeutic effect for pediatric CVA,and its mechanism of action may be related to the regulation of HMGB1-TLR4-NF-κB signal pathway.

Objective The aim of this study is to explore the changes of expression of integrin αvβ3 in bovine u-terine epithelial cells in vitro induced by estrogen or/and progesterone alone or in combination,and to provide a new refer-ence marker for determining bovine uterine receptivity state. Methods RT-PCR was used to analyze the transcriptional changes of αvβ3 expression in bovine endometrium treated by different concentrations of estrogen,progesterone alone or in combination. Results The expression of integrin αvβ3 reached the highest level when the culture medium was added with progesterone at the concentration of 10 -7mg/mL,and the expression of αv and β3 in the 10 -7mg/mL concentration group was significantly higher than that of the control one(P<0.05). Moreover,the expression of αv was highest in the 10 -10 mg/mL E2group,but the expression of β3 was the lowest in that one. In addition,adding with both estrogen and progester-one,the transcriptional level of integrin αvβ3 was significantly higher than that in the control one. The transcriptional level of αv in the treatment group was significantly higher than that in the control group(P<0.05),but the transcriptional level of β3 in this group was not(P>0.05). Conclusions It can be concluded that integrin αvβ3 can be used as a new poten-tial reference marker gene for detecting the bovine uterine receptive status.

A new ST segment analysis scheme is developed for helping the doctors to browse the electrocardiogram (ECG) signals rapidly and then give a correct diagnosis. The preprocessing consists of baseline wander attenuation using median filter, high frequency noise rejection using order statistic filter, and then QRS complexes detection using wavelet analysis. In this paper, after the beginnings and the ends of the ST segments are mutually chosen by doctor, we compute the total deviations between ST segments and the modified baselines using mean value, and then plot the whole trend of the deviations. Doctors can browse the original ECG signals handily by our browsing system, and then give a diagnosis colligating other clinic features of the patient.
Electrocardiography, Ambulatory ; Humans ; Myocardial Ischemia ; diagnosis ; Signal Processing, Computer-Assisted

OBJECTIVE:To observe the effects of clarithromycin and fleroxacin on Pseudomonas aeruginosa(PA) biofilm METHODS:Clinical isolates of 7 strains of PA from respiratory tract were cultured with modified plate culture method;bacterial biofilm model was identified by silver nitrate staining;MICs were determined by broth microdilution The number of viable bacteria in biofilm was measured by using MTT method and bacterial adherence was measured by crystal violet staining RESULTS:1/16MIC and 1/4MIC of clarithromycin could inhibit the adherence of PA to silica-gel film(P

A total of 96 26-week-old Hisex Bro-wn laying hens were divided into 3 groups (32 each) to investigate the effect of niacin on egg production and lipid metabolism of laying hens. Three levels of dietary niacin were used (0, 20 and 60mg/kg diet) for the 8-week experimental period.Supplementation of dietary niacin caused plasma cholesterol, ?-lipoprotein, triglycerides and free fatty acid to decrease 40.9%, 35.7%, 18.2% and 54.3% respectively and liver fat, muscle fat and abdominal fat to decrease 26.7%, 33.4% and 12.3%, respectively, compared with control. Increasing of dietary niacin resulted in a significantly decreases in plasma estradiol (E2) and the activity of hepatic malate dehydrogenase (MD) to regulate lipid matabolism.All of the results demonstrated that niacin could inhibit the lipid synthesis in hen's liver. Niacin deficiency could caused hen liver fragile and yellow in color, liver cells damaged and further fatty liver syndrome in hens.It is suggested that the supplementation of 20mg niacin/kg of diet is appropriate to laying hens if the basal diet contains 0.20% tryptophan and 23.8mg/kg diet of niacin originally.