1.Nasal development of cleft and non-cleft palate in mice
Zhigang CAI ; Helmut DOMARUS VON ; Engel EVELEY ;
Journal of Practical Stomatology 2001;0(03):-
Objective: To study the relationship between the congenital cleft palate and development of the nasal capsule and nasal septum. Methods: 30 pairs of NMIR fetal mouse at the embryo age of 16～17 days were used.In each pair there was one euploid mouse without cleft palate and one trisomy 18 mouse with cleft palate.Sixty maxillae were precisely orientated in the coronal plane and serially sectioned at 7 ?m thickness. With the aid of computer imaging analysis system the width,height and area of the nasal capsule, nasal septum and cartilage of nasal septum were measured and compared quantitatively between the cleft group and non-cleft group. Results: The values of height and area of the nasal capsule, nasal septum and cartilage of nasal septum in cleft foetuses were smaller than those in non-cleft foetuses (P
2.Comparative morphometrical study on development of palatal shelves in cleft and non-cleft palate mice.
Zhi-gang CAI ; Helmut von DOMARUS ; Eveley ENGEL
Chinese Journal of Stomatology 2003;38(3):182-184
OBJECTIVETo quantitatively compare the relationship between the congenital cleft palate and development of the palatal shelf.
METHODSFifty two pairs palatal shelves were macroscopic measured, and 60 series coronal sections were microscopically measured, which were precisely orientated in the coronal plane and serially sectioned at 7 micro m thickness. With the aid of computer imaging analysis system the widths and areas of the palatal shelves in vertical and coronal direction, the maximal areas of the palatal bone and palatal process and alveolar process were measured and compared quantitatively between the cleft group and non-cleft group.
RESULTSThe widths and areas of palatal shelves in cleft foetuses showed significant reduction macroscopically and microscopically as well as the maximal areas of the palatal bone, in addition, both of two processes of the maxilla showed significant developmental deficiency.
CONCLUSIONSThe palatal shelves show significant developmental hypoplasia in three dimension directions, which have significant correlation between palatal cleft and trisomic condition.
Animals ; Cleft Palate ; embryology ; genetics ; Female ; Image Processing, Computer-Assisted ; Mice ; Palate ; anatomy & histology ; embryology ; Trisomy
Result Analysis
Print
Save
E-mail