Objective Investigate the expression difference of miRNA in cerebrospinal fluid (CSF) to explore new biomarkers for glioma diagnosis and evaluate the diagnostic value .Methods The candidate biomarkers in CSF were detected by using FQ-PCR for 20 cases of glioma patients and 20 cases of non-glioma patients(control group) .miRNAs with significant level changes in CSF sam-ples from patients with gliomas (n=20) compared with healthy volunteers (n=20) were screened out by using Mann-Whitney U test and Kruskal-Wallis test ,and the diagnostic values were evaluated by receiver-operating characteristic curves (ROC curves)and area under the curve(AUC) .Results MiR-128 and miR-21 were differentially expressed in CSF samples from patients with gliomas compared with control group .Expression of miR-21 in glioma is significantly higher than that in the control group(P<0 .05) ,while the expression of miR-128 in gliomas was significantly lower than that in control group(P<0 .05) .AUC was 0 .96 when using only miR-21 as the diagnostic biomarker ,and the sensitivity was 90% ,specificity was 95% .The diagnostic sensitivity and specificity were 100% when MiR-128 and miR-21 combined .Conclusion miR-128 and miR-21 are potential markers for gliomas diagnosis in the CSF .

Objective To investigate the potential role of tumor necrosis factor receptor-associated factor-6(TRAF6)in the rat cerebral ischema-reperfusion injury.Methods 40 healthy adult SD rats were divided into 5 groups(n=8)according to the ran-dom control principle:sham operation group,ischemia group,reperfusion 2 h group(R2 h),reperfusion 12 h group(R12 h)and reperfusion 24 h group(R24 h).The rat cerebral ischemia-reperfusion injury model was constructed.The change of TRAF6 expres-sion was examined by RT-PCR and Western-blot.Then,the immunohistochemistry was adopted to locate the TRAF6 protein.Re-sults Compared with the sham group,the expression of TRAF6 in the ischemia group and the R2 h,R12 h and R 24 h groups was obviously increased,but the difference had no statistical significance (P <0.05).TRAF6 was mainly located in the cytoplasm of neuronal cells.Conclusion Activated TRAF6 is involved in the brain cell death induced by cerebral ischemia-reperfusion.

Objective This research is to observe the treatment effects of hip bath and oxygen blowing on severe neonatal diaper rash.Methods 289 neonates with severe diaper rash were randomly divided into three groups.91 cases in the flat tube group,102 cases in the round tube group and 96 cases in the control group.Three groups of neonates were cleaned on the hips and perineuma after poops and then dried with wet tissues and Mupirocin Ointment.The control group was treated with the above-mentioned method.The oxygen blowing groups were treated with hip bath of 1:5000 chameleon solution twice a day.Blowing the hips with oxygen five minutes every time after hip bath.The method of blowing oxygen was that oxygen humidifying containers as normal oxygen aspiration facilities was not filled with water,whose oxygen flowing volume standed at 10 L/min and whose tube blew at the afflicted parts until being dry.The oxygen blower held the oxygen exit and blowed at the afflicted parts in the round tube group and the oxygen blower flattened the oxygen exit and blew at the afflicted parts in the fiat tube group.The treatment effects will be compared among the three groups four days later.Results The cure period of the round tube group was obviously shorter than that of the control group,and the cure period of the fiat tube group was remarkably shorter than that of the round tube group.The total effective rate in the round tube group was obviously higher than that of the control group and the total effective rate in the fiat tube group was obviously higher than that in the round tube group.The difference had a statistical significance.Conclusions The treatment effects for the severe neonatal diaper rash with hip bath and oxygen blowing are remarkable and the oxygen blowing effects with fiat tubes are better than those with round tubes.

AIM: Research report that bone marrow stem cells mobilized by recombinan human granulocyte colony-stimulating factor (rhG-CSF) can migrate to lesion spot of infarction, thus decrease brain edema and brain injury after cerebral ischemia. But the report about the effect of drug on brain edema after cerebral hemorrhage is rare. This study investigated the effects of bone mar- row stem cells mobilized by rhG-CSF on reducing formation of brain edema and downregulation of matrix metalloproteinase (MMP)-9 in the peripheral area after intracerebral hemorrhage (ICH) in rats. METHODS:The experiments were performed at the Central Laboratory of Luzhou Medical College from March to November in 2006. ① 144 healthy male SD rats, (300?20)g, were provided by Animal Department of Luzhou Medical College. The experimental procedures of disposing animals were accorded with ethical standards. ②Experimental rats were assigned randomly into a sham operation group, a ICH group and a treatment group, equally. According to the method of Yang, rat models of ICH were made by the method cutting off tail of rat to obtain autoblood in the ICH and treatment groups. Rats in the sham operation group received saline instead of autoblood. Rats in the treatment group were administered with rhG-CSF (60 ?g/kg) by intrap- eritoneal injection after 1 hour. ③The water contents and MMP-9 were measured in each group by immunohistochemical method. RESULTS:Of 144 rats, 16 rats dropped out, among which 7 rats were estimated as 0 grade and 9 rats died, and all were supple- mented. ①The water contents were higher in the ICH group than in the sham operation group (P

BACKGROUND: Recombinant human granulocyte colony-stimulating factor (rhG-CSF) can mobilize endothelial progenitor cells and enhance new vessels at cerebral ischemia region. OBJECTIVE: To investigate the effects of rhG-CSF on the new microvascular expressions in rat perihematoma after intracerebral hemorrhage. DESIGN, TIME AND SETTING: A randomized control animal experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006. MATERIALS: A total of 72 healthy male Sprague Dawley rats and rhG-CSF were used for this study. METHODS: Seventy-two rats were equally and randomly assigned into the sham operation group, the hemorrhage group, the treatment group. According to rat brain stereotaxic atlas, models of intracerebral hemorrhage were made by infusing autoblood from rat tails. Rats in the sham operation group were infused with saline instead of autoblood. Rats in the treatment group were administered rhG-CSF (60 ?g/kg) by intraperitoneal injection at 1 hour after operation. Rats in the sham operation and hemorrhage groups were left intact. MAIN OUTCOME MEASURES: The microvascular expressions of CD34+ in perihematoma were detected at 6, 12, 24, 48 and 72 hours, 7 days; Four rats in each time point. Microvascular production was measured by changes in CD34. The more the CD34 antigens, the more the new vessels were. RESULTS: In the hemorrhage group, the microvascular expressions of CD34+ were significantly higher compared to the sham operation group (P 0.05). Significant differences were measured at 6, 12, 24 and 48 hours (P

BACKGROUND:Administration of recombinant human granulocyte colony-stimulating factor(rhG-CSF) is known to diminish cerebral edema and to enhance the new vascularization by mobilizing endothelial progenitor cells in cerebral infarction, then to promote the neurofunctional recovery.OBJECTIVE:To investigate the effects of rhG-CSF on brain edema and new vessels after intracerebral hemorrhage(ICH) in rats.DESIGN, TIME AND SETTING:The randomized controlled animal study was performed at the Central Laboratory, Luzhou Medical College from March to November 2006.MATERIALS:A total of 144 healthy male Sprague Dawley rats were equally and randomly assigned into a sham operation group, a ICH group and a treatment group.rhG-CSF(Xinpeng, Shenzhen, China) was used in this study.METHODS:Rat models of ICH were made by the method of cutting off the tail of each rat to obtain autoblood in the ICH and treatment groups.Rats in the sham operation group were injected with saline.Rats in the treatment group were administered with rhG-CSF(60 ?g/kg) by intraperitoneal injection after 1 hour.Eight rats from each group were studied at 6, 12, 24, 48, 72 hours and 7 days.Brain water content of rats was measured by dry-wet method.CD34+ vessel expression was detected by SP and DAB coloration, immunohistochemical method.MAIN OUTCOME MEASURES:Dynamic changes in brain water content;immunohistochemical results of CD34+ vessels.RESULTS:The water contents were significantly higher in the ICH group than in the sham operation group(t=4.49, P

Object To determine the contents of diosgenin in Rhizoma Paridis by HPLC and TLC. Methods ODS column was used in HPLC with mobile phase: acetonitrile water(90∶10), detection wavelength: 203 nm, column temperature: 35 ℃; silica gel plate was used in TLC with developer: cyclohexane ethyl acetate (4∶1), scan wavelength: 610 nm, reference wavelength: 420 nm. Results The linear ranges of HPLC and TLC were 1 2 - 7 2 ?g (r=0 999 8), 0 2 - 1 0 ?g (r=0 995 7). The average recovery and RSD were 102 2%, 3 39% (n=6) and 100 9%, 2 68% (n=6) respectively. Conclusion Both of two methods can be used for the quality control of diosgenin in Rhizoma Paridis.

Objective To investigate the effect of small direct‐current electrical stimulation on migration and invasion related MMPs/TIMPs expression of trophoblast cells .Methods The trophoblast cells were exposed to the direct current electrical field at 150 mV/mm for 5 and 10 hours .Cell images were recorded with continuous photographing and analyzed by image analyzer .The ex‐pression levels of MMP2 ,MMP9 ,TIMP1 and TIMP2 were measured using quantitative RT‐PCR and Western blot .Results In non‐electrical field culture trophoblast cells migrated slowly with random directions .Trophoblast cells cultured in media containing 10% calf serum with the application of 150 mV/mm direct current electrical stimulation ,showed marked cathodal migration (P<0 .01) ,the cell body stretched ,perpendicular to the direction of the electric field .Compared with the non‐electrical field stimulation controls ,trophoblasts under the electrical field stimulation had the increased MMP2 mRNA and protein expression (P< 0 .05) , while MMP9 ,TIMP1 and TIMP2 had no obvious changes of mRNA or protein expressions .Conclusion Physiological direct‐cur‐rent electrical fields might induce directed migration and perpendicular orientation of trophoblast cells .The enhanced MMP2 expres‐sion may play an important role in the migration and invasive activity of trophoblast cells in small electrical field .

BACKGROUND:Astrocytes serve as a major component of central nervous system,which reacted actively to various damages.The astrocytes were strongly expressed with enhanced activity after intracerebral hemorrhage.The pathophysiological significance of this change is presently the research hotspot.OBJECTIVE:To investigate the effects of recombinant human granulocyte colony-stimulating factor(rhG-CSF) on the astrocytes expression after intracerebral hemorrhage.DESIGN,TIME AND SETTING:A randomized control experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006.MATERIALS:Fifty healthy,male,SD rats were randomly divided into sham operation(n=10),model(n=20) and experimental(n=20) groups.The rhG-CSF was purchased from Xinpeng Bioengineering Co.,Ltd.METHODS:According to rat brain stereotaxic atlas,intracerebral hemorrhage model was prepared by the method of cutting off tail to obtain blood.The blood was replaced with physiological saline in the sham operation group.Totally 60 ?g/kg rhG-CSF was administered by intraperitoneal injection at 1 hour after model preparation.There was no injection in the other two groups.MAIN OUTCOME MEASURES:The expression of glial fibrillary acidic protein(GFAP) was detected with with ABC immunohistochemical method at hours 6,24,48,72 and day 7 after intervention.RESULTS:There was not GFAP-positive cell found in the sham operation group.The GFAP was little expressed at 6 hours,increased at 48 hours,and reached a peak at 72 hours(P

BACKGROUND: Recent researches demonstrate that transplantation of bone marrow stem cells in the area of myocardial infarction can directionally differentiate into myocardial cells having normal physiological function and can promote newborn vascularization so as to repair infarction myocardium and improve injured cardiac function.OBJECTIVE: To observe short-term clinical effect of autologous bone marrow stem cell transplantation (ABMSCT) in percutaneous coronary artery on the treatment of acute myocardial infarction.DESIGN: Self-control study.SETTING: Department of Cardiology, Xiangtan Central Hospital.PARTICIPANTS: A total of 27 patients with acute myocardial infarction, including 16 males and 11 females, were selected from Department of Cardiology, Xiangtan Central Hospital from June 2004 to December 2006. Their ages coronary artery was finished after onset emergently; in addition, blood flow of infraction related vessels recovered to grade TIMI3. All patients provided the confirmed consent.METHODS: Operative procedure: All patients were performed with emergently interventional therapy of coronary artery after onset of acute myocardial infarction. One week later, percutaneous cavity tube technique was used to establish infarction related arterial pathway, and guiding filament was used to send micro-perfusion tube into stents. And then,separated bone marrow stem cell suspension was poured through central cavity of micro-tube into the distal end of infarction vessels. Operative evaluation: Dynamic electrocardiogram was evaluated for 24 hours before and after transplantation; in addition, left ventricular ejection fraction and myocardial perfusion defect scores were detected before and at 6 and 12 months after transplantation; otherwise, recovery state and complication were observed in follow up at 6 and 12 months after operation.defect scores: At 6 and 12 months after operation, left ventricular ejection fraction was higher than that before transplantation, and there was significant difference before and after transplantation (P＜0.05). While, myocardial perfusion defect scores were lower than those before transplantation, and there was significant difference before and arrhythmia were not found out, cardiac arrhythmia was not increased, and cardiac arrhythmia combining with malignancy not have any complications and in-stent constriction after operation.