Objective To investigate the effects of Ligustrazine on TNF-α-induced TGF-β and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin di-gestion. Then, the subcultured HPMCs were divided into control group, TNF-α-induced (1 μg/L) group and TNF-α-induced plus low-, medium-and high-dose Ligustrazine (10, 20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-β1 and CTGF mRNAs in HPMCs. TGF-β1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results. Results Ligustrazine significantly decreases TNF-α-induced TGF-β1 and CTGF expression in a dose-dependent manner at both protein and gene levels ( P < 0. 05 ). In addition, medium-and high-dose Ligustrazine injection significantly ameliorates the viability of HPMCs inhibited by TNF-α ( P < 0. 05 ). Conclusion Ligustrazine inhibits expressions of TGF-β and CTGF of HPMCs in an inflammatory conditions.

At present,there are many problems in the cultivation for medical students including lack of humanistic education,insufficient healthy personality shaping and innovation education.We made explorations in aspects of updating education concept,enhancing faculty construction,emphasizing basic course teaching and promoting students' abilities so as to cultivate the comprehensive quality of students majored in medical imaging (ultrasonic medicine).

To test whether Con A has antirejection effect on mouse islet allograft, 600-700 handpicked islets of C57BL/6 mice was transplanted beneath the kidney capsule of the streptozotocin induced diabetic BALB/C recipients. In control group the mean survival time (MST) of the grafts was 14.1?2.4 days. Treatment with Con A significantly prolong the MST of the islet allografts. In group with Con A treatment of the donor islets prior to the transplantation the MST was 25?5.4 days. The MST increased sigificantly after ip injection of Con A to recipient mice. The combined treatment of islet and recipient with Con A resulted in a prolongation of MST to 25.2?4.4 days. The experiments concerning the mechanisms of the antirejection effect of Con A showed that Con A was able to combine with the islet cell membrane well masking perhaps the histocmpatibility antigen. Furthermore the lymphocytes of mice after i. p Con A injection suppressed the lymphocyte proliferation reaction of the svnergic mice to mitogen.

Objective To establish a qualitative and quantitative method for controlling the quality of Radix Puerariae and its preparation. Methods The qualitative fingerprint of effective part (extract part of ethyl acetate) was obtained by using RP-HPLC with methanol-water (42∶58) as mobile phase and UV detection at 250 nm . The quantitative measurement of effective components (puerarin and daidzein) was finished under the above chromatographic conditions. And the 11 kinds of different samples of Radix Puerariae were analyzed by the method. Results Under the qualitative conditions, the 5 common peaks in RP-HPLC fingerprint of the effective part could be used as index peaks for qualitative identification. In RP-HPLC quantitative analysis, the recoveries of the method were 98.0% with RSD of 1.30% for puerarin and 95.4% with RSD of 1.61% for daidzein, respectively. The amount of puerarin and daidzein in Radix Puerariae was 0.213%～3.626% and 0.012%～ 0.049% , respectively. Conclusion The analytical method with qualitative fingerprint of effective part and quantitative measurement of effective components of Radix Puerariae can be accurately used for controlling its quality.

The vessels of the skin flap in the fronto-parieto-temporal region were studied by way of injecting 10% ethyl acetate dye into the artery of 25 Chinese adult cadavers.The main arterial supply in this region is the superficial temporal artery. In addition,the posterior auricular,supraorbital and supratrochtear arteries distribute over this region as well.The superficial temporal artery is the largest scalp artery.The area distributed by this artery amounts to 57.38?6.05% of the whole scalp.The frontal and parietal branches are the terminal branches of the superficial temporal artery.The former is a constant branch in our cases.It is a dominant vessel of which both the distributing area and the caliber are larger than the parietal branch.The superficial temporal vein is mainly formed by the union of a frontal and a parietal venous branches,which approximately accompany the corresponding branches of the artery. The arteries in the fronto-parieto-temporal region form rich anastomoses at the median area of the vertex.The large anastomoses channels were counted in this study.These channels have anatomically provided for the designing of a supe(?)long flap which run across the median zone.The forehead flap with the frontal branch as the pedicle artery and the scalp flap with the parietal branch as the pedicle artery were discussed.

Alteration in gastric emptying may play a role in the pathogenesis of many diseases.And various methods to measure gastric emptying have been developed.Nine procedures for the assessment of gastric emptying were reviewed in this paper including intubation test,radiology method,absorption test,real time ultrasound,breath test,radionuclide technique et al .Among all of these methods,radionuclide technique is considered as the good standard method.

Recent developments on separation and analysis of bacterial cells by capillary electrophoresis are reviewed. Based on bacterial surface characterization, capillary electrophoresis technique can be utilized for rapidly identify and quantify intact bacteria and microbes at a certain phase. The microbe-molecule interactions can also be described. The separation of microbes that can not be cultured in lab appears to be possible by this method. It makes profound effects on separation science and microbiology to establish capillary electrophoresis procedure for the separation and analysis of bacterial cells.

0.25mg/ml of Achyranthes bidentata Saponins( ABS ) in De Jalon's solution usually caused an obvious excitant action in both virgin and pregnant uterine preparations. The uterine excitation was blocked effectively by prior treatment of the preparations with 20?g/ml of indomethacin for 1 h.In the isolated uterine preparations taken from the rats treated with indomethacin, 15 mg/rat orally twice a day for 2 and a half days, the excitant action of ABS was obviously reduced.Treatment of the uterine segments with 0.5?g/ml of chlorpromazine for half an hour also blocked the excitant response. However, treatment of the uterine preparations with 10?g/ml of atropine for 10 min had no abvious effect on the excitant response. The probable mecha-nism of action of ABS was discussed.

Objective To compare the objective cure rate and perioperative complications 0f ten-sion free vaginal tape(TVT)and laparoscopic Burch for the treatment of fernale stress urinarv inconti-nence. Methods Literature search was conducted for all randomized control trials(RCTs)between 1994 and 2006,using MEDLINE,EMBASE with the keyword"tension free vaginal tape","colpo-suspension","stress urinary incontinence",then a second search was performed with the following keywords:[(tension free vaginal tape),AND(colposuspension),OR(Burch)],AND stress urinary incontinence].Gray literature was search in The Cochrane central register of controlled trials、NRR (National Research Register)、CCT(clinical controlled trials),ISTP,ISI proceedings,OCLC First-search proceedings,(CACP),GreyNet(http://www.greynet.org/)、DARE(Database of Abstract:s of Reviews of Effects)、SIGLE(System for Information on Grey Literature in Europc),official website of ICS.Meta-analysis was performed with RevMan software 4.2. Results right studies were included comparing objective cure rate and perioperative complications of TVT and LB.Overall objective cure rates were 233/259 for TVT and 189/247 for Burch respectively.TVT shared higher objective cure (RR 1.19,95%CI 1.10-1.29,P<0.01)and lower perioperative complications(RR 0.48,95%CI 0.32-0.71,P<0.05). Conclusions The available evidence indicates that TVT is an effective treat-ment for stress urinary incontinence with higher objective cure rate and 10wer perioperative complica-tions compared with LB.

Objective To observe the changes of serum 25‐hydroxy vitamin D[25‐(OH)D] level of patients with rheumatoid ar‐thritis(RA) and study its clinical significance .Methods The method of chemiluminescence was adopted to detect peripheral blood serum 25‐(OH) D of 60 cases of RA patients(RA group) and 60 cases of healthy people(control group) .Results The serum 25‐(OH) D level of patients in RA group was(34 .37 ± 8 .87) nmol/L ,while that of the control group was(61 .33 ± 7 .74) nmol/L ;after comparison ,the differences were statistically significant (t= -6 .892 ,P< 0 .05);the serum 25‐(OH) D level of patients in RA group was in negative correlation with RA patient age ,body mass index(BMI) ,time of morning stiffness ,tender joint count(TJC) , swollen joint count(SJC) ,C‐reactive protein(CRP) ,erythrocyte sedimentation rate(ESR) ,visual analog scale(VAS) for disease o‐verall pain and health assessment questionnaire (HAQ) and disease activity score (DAS28)(r= -0 .201 ,-0 .255 ,-0 .362 ,-0 .296 ,-0 .273 ,-0 .198 ,-0 .323 ,-0 .356 ,-0 .379 ,-0 .418 ;P<0 .05 in all cases);but had no correlation with course of dis‐ease ,rheumatoid factor (RF) ,anti‐cyclic citrullinated peptide (CCP) antibody (anti‐CCP) and antibody concentration (P> 0 .05) . Conclusion The serum 25‐(OH) D level of RA patients is significantly low ,which might be associated with the RA disease activi‐ty .