Abstracting and Indexing as Topic ; Medicine, Chinese Traditional ; Terminology as Topic ; Translations

A simpler method for preparing hap- toglobin from the waste residue of Rivanol- ammonium sulphate fractionation of human serum during the production of human immunoglobulin is described. The agar do- uble diffusion test shows that the large amount of haptoglobin can be prepared by this procedure and the immunoelectropho- resis analysis demonstrated specific preci- pitated line with the reference anti-hapto- globin antibody, showing that the protein molecule of haptoglobin is homogeneous.

As an important device of modern medical treatment,medical monitor is a combination of modern electronics technology,computer technology and medical technology.In this paper,the development of medical monitors in China is summarized,especially the main technique characteristics of medical monitor in Chinese market.The examples of monitors made in China are presented.

Objective To investigate the extraction technique for atractylenolide Ⅰ in Atractylodes macrocephala by supercritical CO_2 fluid extraction and develop a method used for determining the content of atractylenolide Ⅰ in the extract by HPLC. Methods The effects of seven facters, such as the extracting pressure, resolving pressure etc, to the extraction rate of atractylenolide Ⅰ in A. macrocephala by supercritical CO_2 extraction were investigated. RP-HPLC was used to determine the content of atractylenolide Ⅰ in extraction of A. macrocephala. The separation was performed on Hypersil ODS2 column with methanol-water (70∶30) as mobile phase. The flow rate was 1.0 mL/min and the wavelength of UV detector was 220 nm. Results The optimal extracting conditions: taking 10% alcohol as entraiter, the particle size of medicinal substances was 60 screen meshes, extracting pressure 25 MPa, resolving pressure 5 MPa, extracting temperature 40 ℃, resolving temperature 30 ℃, and the extracting time 4 h. Conclusion Supercritical extraction is time-shorter and efficient in extracting atractylenolide Ⅰ from A. macrocephala. It is suitable to both trial and industrialized production. The method established to determine the content of atractylenolide Ⅰ of A. macrocephala by supercritical extraction is simple, sensitive, and reliable.

OBJECTIVE: To study the apoptosis/proliferation of Kölliker organ supporting cells and to understand the prompting apoptosis factors in vivo in the supporting cells in the Kölliker organ by changing the environment of the cultured supporting cells in the Kliker organ in vitro, via the separation, culture and purification of the supporting cells in the K6lliker organ. METHOD: A combinatorial approach of enzymatic digestion and mechanical separation was employed to isolate and culture in vitro pure Kölliker organ supporting cells. The purity was tested by flow cytometry assay. And K6lliker organ supporting cells were harvested to detect the rate and cycle of apoptosis by flow cytometry after Annexin V/PI staining, to test the cell growth curve by MTT assay, and to observe the differential expressions of the Bcl-2, Caspase-3, Caspase-8 and Caspase-9 through the Realtime PCR and Western blot. The calcium, potassium and glutamate concentrations in the culture medium of these cells in vitro were changed to detect the survival rate of cells by MTT assay. RESULT: The purity of K6lliker organ supporting cells by flow cytometry assay was 96. 56%. And these cells showed no significant difference in apoptosis, but an evident linear growth. The results of Realtime PCR and Western blot showed that the expression of Bcl-2, Caspase-3, Caspase-8 and Caspase-9 mRNA and protein in all different time points kept stable. Furthermore, the elevation of extracellular Ca2+ might contribute to decrease the cell viability of supporting cells. And K+ participated regulation of cell viability in a concentration-depending way. However, glutamate appeared to be a protective factor in high concentration. CONCLUSION There is no significant apoptosis in vitro of the supporting cells in the Kölliker organ of rats, showing a linear growth. The Ca2+ in high concentration might contribute to the apoptosis factor of these cells. However, the K+ and glutamate appear to be protective factors in high concentration.
Animals ; Animals, Newborn ; Apoptosis ; Caspase 3 ; Cell Cycle ; Cell Proliferation ; Cell Survival ; Cochlea ; cytology ; growth & development ; Flow Cytometry ; In Vitro Techniques ; Rats

Objective To describe the imaging of the vagina massive hemorrhage patients,which is caused by pero-abortion by the high-frequency ultrasonic examination,and to establish the character of ultrasound.Methods 56 patients,who have the vagina massive hemorrhage after the drug abortion,were checked by the high-frequency ultrasonic examination,to observe if there are fetus in the uterus.Results There all have fetus in the uterus in 56 patients by the high-frequency ultrasonic examination.Including 8 patients who have little residues;22 patients have residues complic fluidify in the uterus;26 patients have admix residues.Conclusions After the drug abortion,the vagina massive hemorrhage,which is caused by pero-abortion,is the severity complication endangering the life of the patients,we cound found the pero-abortion early by the high-frequency ultrasonic examination and clinical observe.

Objective To reproduce the human endometriosis (EMS) in nude mouse, and to asssess the effects of endostatin in this model. Methods Endometrium tissue was collected from four women undergoing hysterectomy for EMS, and it was transplanted into 30 female BALB/c nude mice by subcutaneous injection (n=20) and intraperitoneal injection (n=10) respectively. The development of endometriotic lesions in nude mice was observed three times every week. Two weeks later, the successful rate in the two groups was estimated. The model mice in subcutaneous injection group were then randomized into treatment group (n=8) and control group (n=7). The mice in treatment group were injected with human endostatin (2mg?kg -1 ?d -1 ), while the mice in control group received an equivalent volume of PBS. All mice were sacrificed 14 days after treatment. Immunohistochemistry was used to determine the expression of vascular endothelial growth factor (VEGF). Results Transplantation was successful in 7 nude mice in abdominal implantation group and 15 nude mice in subcutaneous injection group. The success rate was 75% in subcutaneous injection group and 70% in abdominal implantation group, no significant difference was found between two groups. The level of VEGF in the nude mice treated with endostatin was significantly lower than that in the control group (P

Atrial fibrillation is the most common arrhythmia disease in the clinical works,and endangers the public health seriously.With the development of AAD and ABL,we have made a great progress in AF's prevention and treatment.However,the literature and research about the non-disease causes of lone AF are very rare.So,the article will make a system review and summary about it,in order to instruct the lone AF's primary prevention.

Objective To discuss the practical significance and clinical therapeutic effects of shoulder arthroscopy technique in the diagnosis and treatment of glenoid labrum injury. Methods In order to have an accurate diagnosis of glenoid labrum injury of the shoulder, X-ray, arthrography, MRI and arthrog-raphy and arthroscopy techniques were used respectively for thirty-six cases of glenoid labrum injury. Under shoulder arthroscopy, the injured glenoid labrum was partially resected in twenty-eight patients affected with single glenoid labrum injury. The injured glenoid labrum of five SLAP cases were treated with debridement under shoulder arthroscopy, and then the affected shoulder was immobilized by zero-degree traction. The injured glenoid labrum of three Bankart cases also underwent debridement under shoulder arthroscopy, and then treated with improved Bristow operation through limited incision. Results After 2-year follow-up, according to the standard criterion of U. S. Michasel Reese Medical Center, the total pain score and the total joint function score were 75 and 25 respectively; the patients' average score was 65 and 20 postoperatively, compared with 35 and 10 preoperatively. The clinical symptoms of all these cases were greatly improved and the unstable symptoms disappeared postoperatively in eight SLAP injury or Bankart injury patients and they all returned their preoperative activities. Conclusion Glenoid labrum is one of the most frequent parts of shoulder joint to be injured. Shoulder arthroscopy is a necessary and effective advanced technique for a definite diagnosis and treatment of glenoid labrum injury. There is some difficulty to carry out operation with arthroscopy and some specific instruments, however, it is necessary for the surgeon to understand and handle the technique.

Traumatic deep vein thrombosis is a venous thrombotic disease which are secondary to trauma or surgery.This thrombotic disease is the most common clinical complication in surgery,especially in orthopedic surgery.The article overviews recent prediction indicators of academic value on traumatic deep vein thrombosis from six facts contain gene,inflammation,coagulation factor,supplementary examination,lipid molecules and selectin.These new indicators will play a more active role in prediction and treatment for traumatic deep vein thrombosis.