ObjectiveTo explore the effect of Curcumin on TGF-β1 and HGF in pulmonary fibrosis and the effect of different concentrations of Curcumin on the expression of HGF and TGF-β1 in pulmonary fibrosis.Methods90 health male SD rats were randomly divided into six groups as follows:the control group ( group A),model group ( group B),prednisolone-treated group ( group C),and groups of low ( D group),middle( group E) and high( group F) dose curcumin (50,100,and 200 mg/kg,respectively).Animal model of pulmonary fibrosis was produced in SD rats by the endotracheal bleomycin,samples were obtained on days 7,14,and 28.The expression of HGF and TGF-β1 were measured by RT-PCR and Westem blot.ResultsThe light density values (0.693 ±0.028) and gray value (0.96 ±0.10)of TGF-β1 on day 28 of group A was the lowest,while the light density values ( 1.586 ±0.020) and gray value ( 1.77 ±0.15 ) of group B was the highest,the light density values (0.881 ± 0.032) and gray value ( 1.19 ±+ 0.12 )of group F was lower than group B.But the light density value and gray value of HGF showed reverse,the light density values and gray value of HGF on day 28 of group F was the highest,there was no statistical difference between group B and group D ( P ＞ 0.05).ConclusionsThe protective effect of Cucumin against pulmonary fibrosis may be through stimulating the activity of HGF and inhibiting the activity of TGFβ1 in a dose-dependent manner.

Humans ; Macrophage Activation Syndrome ; diagnosis

BACKGROUND: Previous research has indicated that bone marrow-derived mesenchymal stem cells had immunological regulation and hematopoiesis role. There were significant differences in immunological regulation and hemopoietic function between patients with aplastic anemia and normal cases. OBJECTIVE: To summarize the in vitro biological characteristics and its immune regulation defects of bone marrow-derived mesenchymal stem cells from patients with aplastic anemia.METHODS: A computer-based online search was conducted in PUMMED (1987-2009) and VIP database (1989-2009) with the key words of "mesenchymal stem cell, bone marrow" in both Chinese and English. There were 55 articles in total. Articles which were related to biological characteristics and its immune regulation defects of bone marrow-derived mesenchymal stem cells were included; however, duplicated articles were excluded. Finally, 32 articles were included, containing 3 reviews in English, 23 original articles in English, and 6 original articles in Chinese. RESULTS AND CONCLUSION: Bone marrow-derived mesenchymal stem cells had the capacities of high proliferation, self-renewal and multilineage differentiation; in addition they had the roles of supporting hematopoiesis, promoting implantation and hematopoietic reconstitution in vivo. Bone marrow-derived mesenchymal stem cells still had the effects of negative immune regulation and reducing the incidence of graft-versus-host disease (GVHD). Aplastic anemia correlated with hematopoietic stem cells in the pathogenesis of intrinsic defects in the proliferation or differentiation of hematopoietic microenvironment and immune system abnormalities and other related disorders. Bone marrow-derived mesenchymal stem cells of patients with aplastic anemia played an important role in the pathogenesis of this disease.

Arthritis, Juvenile ; diagnosis ; Child ; Forecasting ; Humans ; Immunologic Tests ; Lupus Erythematosus, Systemic ; diagnosis ; Mucocutaneous Lymph Node Syndrome ; diagnosis ; Rheumatic Diseases ; diagnosis ; immunology ; therapy ; Rheumatic Fever ; diagnosis ; Scleroderma, Systemic ; diagnosis

Alzheimer's disease (AD) is a most common neurodegenerative disease. The mechanisms underlying AD, especially late-onset AD, remain elusive. In the past few years, results from genome-wide association studies (GWAS) and systems approaches indicated that innate immune responses mediated by microglia played critical roles in AD. Functional analysis on animal models also showed that immune receptors or proteins expressed in microglia mediated Abeta-induced inflammation, or Abeta phagocytosis by microglia. Microglia plays double sword roles in AD. More work is warranted to elucidate the exact roles of microglia in AD, which will facilitate our better understanding of the mechanisms underlying AD.

Urinary calculus is a common disease of urinary system.With the improvement of people's living standard,the incidence rate of urinary calculus increased year by year.Compared with other methods,spiral CT has a great advantage in the diagnosis of urinary calculus.The current commonly used examinations including spiral CT plain scan,low dose spiral CT scan,CT urography,and dual source dual energy CT imaging.The advantages and characteristics of different types of CT are analyzed and compared,which is helpful for clinicians to choose the most proper examination,to make the most of the advantages of various checks,so as to improve the accuracy of diagnosis and effectiveness of treatment.

The paper introduces the survey on the current conditions of higher stomatolo-gy education in Papua New Guinea which locates in the south Pacific and is slow on some as-pects,however it has its own characteristics worth of using for reference.The paper also ana-lyzes the disadvantage of teaching system in China and puts forward some reform consideration based on our real situation.

ObjectiveTo understand dangerous factors causing child cerebral palsy and try to diagnose and treat cerebral palsy early, and decrease the ratio of cripple.MethodsTo analyze if the occurrence, style or complication of cerebral palsy having a relation to dangerous factors such as sexes, birth weight, premature delivery, twins, neonatal asphyxia, infection of newborn, nuclear icterus, mother's age, virus infection in the duration of pregnancy, threatened abortion and family history of cerebral palsy.ResultsThe ration of male and female is 1.47∶1 in 146 cases of child cerebral palsy. Factors causing cerebral palsy are low birth weight (79.45%), premature delivery (64.38%), neonatal asphyxia (41.78%), threatened abortion (32.89%), twins (31.53%), infection of newborn (12.33%), nuclear icterus (6.16%), parturient with advanced age (3.42%), having infection history in the duration of pregnancy (2.74%), and family history of cerebral palsy (0.68%). Both styles and complications of cerebral palsy have a relation to dangerous factors.ConclusionsThe low birth weight, premature delivery, neonatal asphyxia, twins and threatened abortion all are higher dangerous factors causing child cerebral palsy. In order to abstain the occurrence of these dangerous factors, it is important to educate people and doctors recognizing all dangerous factors. It is also helpful to lower the ratio of cripple that regularly examining child with dangerous factors, discovering symptoms and complications of cerebral palsy in time, and diagnosing and treating cerebral palsy as soon as possible.

Objective To observe the effect of pulsed electromagnetic fields (PEMFs) of different intensities on the biomechanical properties of the femur in ovariectomized rats so as to determine the intensity for the best therapeutic efficacy. Methods Fifty female Sprague-Dawley rats were randomly divided into (1) a sham-operated control group (no PEMF treatment) , (2) ovariectomized control group (no PEMF treatment) (3) ovariectomized group Ⅰ (PEMF treatment at 8 Hz and 0.77 mT intensity, 40 min daily for 30 days) (4) ovariectomized group Ⅱ (PEMF treatment at 8 Hz and 3.82 mT intensity, 40 min daily for 30 days) and (5) ovariectomized group Ⅲ( PEMF treatment at 8 Hz and 9.87 mT intensity, 40 min daily for 30 days). Except for the 10 rats of the sham-operated control group, all the others received a standard ovariectomy. Serum estradiol (E2) and the biomechanical properties of one femur (peak load, maximum displacement, maximum energy absorption, maximum stress, maximum strain and modulus of elasticity) were assessed after 30 days of PEMF treatment. Results In group Ⅱ the biomechanical properties of the femur were significantly better than in group Ⅰ or the ovariectomized control group. In groups Ⅰ and Ⅲ the biomechanical properties of the femur were not significantly better than in the sham-operated group. In group Ⅱ the biomechanical properties of the femur were significantly better than in groups Ⅰ or Ⅲ. Conclusion PEMFs at 3.82 mT can improve the biomechanical properties of the femur significantly.

Animals ; Antineoplastic Agents ; therapeutic use ; Benzamides ; Dasatinib ; Drug Resistance, Neoplasm ; drug effects ; Fusion Proteins, bcr-abl ; genetics ; metabolism ; Humans ; Imatinib Mesylate ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; drug therapy ; genetics ; metabolism ; Piperazines ; therapeutic use ; Protein-Tyrosine Kinases ; antagonists & inhibitors ; Pyrimidines ; pharmacology ; therapeutic use ; Thiazoles ; pharmacology ; therapeutic use