Objective To observe if IL-2 gene can express in hepatic carcinoma cells by double targeting of hepatitis B virus envelope (HBVE) and alpha fetoprotein (AFP) promoter.Methods HepG2 cells,L02 cells,and HepG2.2.15 cells were cultured in vitro.HBVE was obtained by PEG8000 concentration assay,and the acquired HBVE was used to pack recombintional gene.Human AFP promoter-IL-2 recombinational gene was obtained by PCR.Then HepG2 cells and L02 cells were transfected by transient transfection and the expression of IL-2 was detected by RT-PCR and Western blot.Results IL-2 was detected in HepG2 cells by RT-PCR and Western blot but not in L02 cells.Conclusion By using HBVE as a gene transporter,human AFP promoter-IL-2 recombinational gene can express in hepatic carcinoma cells,thereby it can increase the safety of exogenous gene transfection of hepatic carcinoma cells.

The incidence of upper limb dysfunction of stroke patients was high, and the recovery was difficult, which seriously impact-ed their activities of daily living and quality of life. This article reviewed the new techniques and methods for hemiplegic patients after stroke. The main techniques and methods includeed bilateral isokine-matic training, mirror neuron system, gymnastics rods training, music therapy, motor imagery, upper limb rehabilitation robot, virtual reality, constraint induced movement therapy, functional electrostimulation, electromyographic biofeedback therapy, transcranial magnetic stimulation, neurophyisological therapy and so on.

Phosphatase of regenerating liver-3 (PRL-3) is a novel small molecule protein-tyrosine-phosphatase,which plays an important role in the oncogenesis and deveopment of tumors.Studies show that PRL-3 regulates neoplasm progress through participating in multiple signal pathways.Its high expression can significantly promote neoplasm metastasis in cancer tissue.However,there is no expression of PRL-3 in most normal tissue.PRL-3 is expected to be a potential tumor maker of diagnosis and a new target for the therapy of cancer.

Objective　To observe the presence of synergistic efficacy between adenovirus mediated bcl-Xs (Adv-bcl-Xs) gene therapy and chemotherapy on ovarian cancer cell. Methods　NuTu-19 cells were infected by different titers of Adv-bcl-Xs and were treated with topotecan at the same time. Cell proliferation was measured 3 days later by MTT. Graphical representations of the statistical analyses recorded their interaction in tumor cells. Results　The statistical results and graphical representations of the statistical modeling showed that the synergistic antiproliferative activity was present (P＜0.01). Conclusion　There were synergistic efficacies between Adv-bcl-Xs gene therapy and Topotecan on ovarian cancer cell.

Objective To observe the evolution process of cerebral blood flow spectrum in brain death by transcranial doppler ultrasound.Methods To detect the blood flow of bilateral middle cerebral artery in brain dead patients by transcranial doppler ultrasound.Results Twenty brain dead patients were collected in the study.The blood flow velocity of middle cerebral artery ranged s from 6 to 37cm/s,and the mean velocity was (12.13 ± 3.15)cm/s.There were three characteristic spectrums which include oscillatory wave (reverse wave in diastolic),nails wave (tip small wave in systolic),no blood flow sign,and they appeared in turn accoerding to worsen of the disease.The heartbeat irreversibly stopped within 8 days after the appearing of oscillatory wave,and ninty percent of the cases had the regular blood flow spectrums of oscillatory wave-nails wave-no blood flow sign.Conclusion The three regularly characteristic transcranial doppler ultrasound blood flow spectrums,which include oscillatory wave,nails wave and no blood flow sign could facilitate the diagnosis of brain death.

ObjectiveTo explore the scientific and effective management of subsidization for students in poverty and to build a rational, scientific-supporting system of poor students. MethodsAnalyzing the data of the poverty status and funding, of poor students in 2006-2009 in an independent Institute ResultsAmong all the means of subsidization for students in poverty, study aid covers most and the number of students who get this aid is increasing while there are fewer national scholarships and living subsidies. ConclusionSupporting system of poor students is not perfect enough, and institutions of higher learning should find the practical way to help our poor students complete their studies successfully.

Objective To assess the predictive value of cerebral arteriosclerosis to acute cerebral infarction evaluated by transcranial doppler ultrasound .Methods 170 cases suspected cerebral arteriosclerosis consecutively enrolled were given transcranial doppler ultrasound and were followed up for 6 months,then we assessed the correla-tion between the levels of cerebral arteriosclerosis and acute cerebral infarction within 6 months.Results By Spearman rank correlation analysis ,there was positive correlation between the severity of cerebral arteriosclerosis de-tected by transcranial doppler ultrasound and acute cerebral infarction within 6 months,the correlation coefficient was 0.575(P<0.05).Conclusion Through the detection of transcranial doppler ultrasound ,the higher levels of cere-bral arteriosclerosis,the greater probability of acute cerebral infarction within 6 months.

BACKGROUND:Osteoporosis is a condition resulting in an increased risk of skeletal fractures due to a reduction in bone mass and degradation in bone microstructure. Treatment of osteoporosis usual y involves the application of pharmacological agents as the first choice. Pharmacotherapy alone is not sufficient for the comprehensive management of osteoporosis, as pharmacotherapy has no effect on increasing muscle strength, improving balance ability, and preventing fal s. Exercise, considered as an important non-pharmacologic therapy, plays a considerable role in the prevention of osteoporosis. The importance of therapeutic exercises in the treatment of osteoporosis has been recognized gradual y.
OBJECTIVE:To discuss the effect of exercise in the treatment of osteoporosis combined with some of the latest research literatures.
METHODS:Clinical reports and mechanism researches about effects of different types of exercise on bone strength especial y the treatment of osteoporosis were retrieved from CNKI database and Medline database with the key words of“osteoporosis, bone mineral density, bone strength, treatment, exercise, stress, tai chi, muscle, bone architecture”in both Chinese and English from January 2001 to February 2013. The repetitive researches and atypical reports were eliminated.
RESULTS AND CONCLUSLON:Therapeutic exercises including aerobic exercise, resistance, impact movement, and vibration, which can safely increase bone strength and muscle strength, improve equilibrium function, and prevent fal s and fractures. For spinal deformity patients, appropriate orthotics can improve the security and promote exercise therapy. As with drug therapy, therapeutic exercises are also individualized. Exercise program should be selected under the insurance of good compliance and security. Exercises that involve high strain rates seem to be more effective than others. However, al these benefits are comparatively smal , and should be maintained by continuous exercises.

Apoptin,a chicken anemia virus-derived protein.selectively induces apoptosis in trans-formed or tumor cells but not in normal cells.The tumor specificity of apoptin is thought to be related to its cel-lular localization.The apoptin-induced apoptosis is p53-independent and can not be blocked by overexpressionof Bcl-2.But activation of Caspase-3 is essential for apoptin-induced apoptosis.These features make apoptin a potential candidate as a novel therapeutic and diagnostic tool in cancer treatment.

OBJECTIVE: To study the classification of common symptoms and the laws of syndrome element combination in 251 cases of stable angina pectoris (SAP) by using cluster analysis and corresponding-correlation analysis. METHODS: A total of 251 SAP cases were selected and their information from four diagnosis in traditional Chinese medicine was recorded. The classification of common symptom and the laws of syndrome element combination were investigated by cluster analysis and corresponding-correlation analysis. RESULTS: Twenty-five symptoms found in 251 SAP cases were divided into four types by cluster analysis: deficiency of heart qi, deficiency of spleen qi, deficiency of qi and yin, and phlegm accumulation and blood stasis. The deficiency of heart qi had the closest relation to phlegm accumulation and blood stasis. By corresponding-correlation analysis, the deficiency of qi had the closest relation to blood stasis, next was turbid phlegm and heat stagnation, and then deficiency of qi and deficiency of yin. CONCLUSION: Blood stasis due to deficient qi is the key factor in pathogenesis of SAP. Deficiency of qi plus blood stasis, deficiency of qi plus deficiency of yin, blood stasis plus turbid phlegm, deficiency of heart qi plus blood stasis plus turbid phlegm are common syndrome element combinations of SAP. It is proved that cluster analysis and corresponding-correlation analysis are the proper methods for studying laws of syndrome element combination.