Objective To investigate the parameter of technical shaping process of Muxiang Shunqi Wan and select appropriate auxiliary materials to enhance the time limit of dissolution. Method Select parameter of technical shaping process by orthogonal test and choose the best dosage of disintegrant by well-proportioned design. Results The best preparation and technical shaping process of Muxiang Shunqi Wan is as follows:the drug powder is refined with CMS-Na 1.4%, CCNa 0.5%, 60 ℃ dry. Conclusion Pills made by this technical process appear round and smooth, the colour and luster are equal and conformable, the dissolving time limit is short and the quality is good, therefore, they are up to the GMP requirements.

Epithelial-mesenchymal transition is closely related with invasion and metastasis of malig-nant tumor and is focused on invasion and metastasis in malignant tumor in recent years .Many researches dermon-strate that epithelial-mesenchymal transition is involved in invasion and metastasis of several malignant tumors , such as breast cancer ,ovary cancer and non -small cell lung cancer .The relevant signal pathways and molecular proteins are also studied .Research progress of epithelial -mesenchymal transition in non -small cell lung cancer for invasion and metastasis is reviewed in this article .

OBJECTIVE:To study the mechanisms of GJKL(Gujikang liniment) in treatment of soft tissue injury METHO_DS:The effects of intraperitoneal injection of acetic acid solution on capillary permeability were observed;An animal model of soft tissue injury was established by striking The effects of GJKL on hemorheological parameters were observed RESULTS:GJKL markedly inhibited capillary permeability and decreased hemorheological parameters CONCLUSION:GJKL takes therapeutic effects on soft tissue injury through inhibiting the increased capillary permeability and reducing the hemorheological parameters

Mast cells (MCs) play a key role in the pat hogenesis of allergic diseases. Tissue MCs are originated from hematopoietic ste m cells in bone marrow. In recent years, it was reported that human mast cells c ould be differentiated from stem cells of umbilical cord blood. In this review, we summarize the development in this novel area.

AIM: To investigate the actions of trypsi n on the secretion of monocyte chemoattractant protein-1 (MCP-1) from human lung e pithelial cells. METHODS: A549 cells were cultured in a 12-well culture plate. Th e challenge was performed by addition of various concentrations of trypsin or tr ypsin inhibitor into each well, respectively. After 2 h, 8 h or 16 h, the reacti ons were terminated by removal of the supernatant from each well. A sandwich ELI SA was used to determine the levels of MCP-1 in supernatants. RESULTS: Following 16 h incubation, trypsin was able to induce c oncentration-dependent secretion of MCP-1. As low as 3 ?g/L trypsin was able to induce MCP-1 release from epithelial cells, and the maximum of accumulated rele ase of MCP-1 was observed with 100 ?g/L trypsin, which was 3 fold more than bas eline release. However, trypsin at 300 ?g/L did not induce significant MCP-1 se cretion. Soybean trypsin inhibitor (SBTI) inhibited trypsin-induced MCP-1 secret ion, but ? 1-antitrysin (? 1-AT) did not. The time course showed that the actions of trypsin initiated at 2 h and reached their peak at 16 h. CONCLUSION: Trypsin is a potent secretogogue of MCP-1 release fr om cultured human lung epithelial cells, and itself action can be inhibited by S BTI.

The fluid status variate sharply in patients after operation. Appropriate fluid therapy is essential to reduce perioperative complications, shorten hospitalization days and improve prognosis of postoperative patients. In this article, we reviewed the relevant issues about fluid therapy strategy for postoperative patients such as the variation of body fluid in patients after operation, the assessment of fluid status, the establishment of the goal of fluid therapy and the selection of strategy or fluid for fluid therapy in patients after operation.

Gastric cancer is common in the world. Only 40 % of the patients can be treated by radical operation. Even for those patients who undergo radical resection, the rate of recurrence or distant metastasis is high. To increase the chance of curative surgery and improve survival for gastric cancer, combined-modality treatments have been investigated. There are different therapeutic modes, chemotherapeutic regimens and treatment results between Eastern countries and Western countries. The current status and advance on the adjuvant and neo-adjuvant treatment of gastric cancer were reported.

Apoptin,a chicken anemia virus-derived protein.selectively induces apoptosis in trans-formed or tumor cells but not in normal cells.The tumor specificity of apoptin is thought to be related to its cel-lular localization.The apoptin-induced apoptosis is p53-independent and can not be blocked by overexpressionof Bcl-2.But activation of Caspase-3 is essential for apoptin-induced apoptosis.These features make apoptin a potential candidate as a novel therapeutic and diagnostic tool in cancer treatment.

Phosphatase of regenerating liver-3 (PRL-3) is a novel small molecule protein-tyrosine-phosphatase,which plays an important role in the oncogenesis and deveopment of tumors.Studies show that PRL-3 regulates neoplasm progress through participating in multiple signal pathways.Its high expression can significantly promote neoplasm metastasis in cancer tissue.However,there is no expression of PRL-3 in most normal tissue.PRL-3 is expected to be a potential tumor maker of diagnosis and a new target for the therapy of cancer.

Glechoma hederocea agglutinin (Gleheda) is a novel glycosylated lectin isolated from the leaves of G. hederacea. Like other glycosylated proteins, the detection of Gleheda by immunological methods is often hampered by the cross-reactivity of the polyclonal antibodies with unrelated glycoproteins. Hence a protocol to purify monospecific polyclonal antibodies from a crude antiserum raised against Gleheda was developed. After selective ammonium sulfate precipitation and successive affinity chromatography on columns of Sepharose 4B with immobilized Gleheda and Robinia pseudoacacia agglutinin (RPA), respectively, ion-exchange chromatography on a column of Q Fast Flow was used for further purification. The specificity of the antibody fractions from each step was tested by double immunodiffusion assay and analyzed by Western blot. Results revealed that affinity chromatography of the immunoglobulin fraction on the immobilized Gleheda antigen yielded an antibody preparation that still cross-reacted with many proteins in leaf extracts. Depletion of nonspecific cross-reacting antibodies directed against the glycan part of the glycoprotein by affinity chromatography on immobilized RPA removed most but not all nonspecifically reacting antibodies. Only upon further purification by ion exchange chromatography an IgG fraction of monospecific antibodies that reacted exclusively with Gleheda could be obtained and accordingly was suitable for immunodetection studies. This antibody purification procedure promises simplicity and efficiency. In addition, this method does not require expensive facilities.