Objective To observe the therapeutic effect of oral use and external application of Chinese herbal decoction for the treatment of recurrent condyloma acuminatum(RCA).Methods One hundred and twenty-eight RCA patients were divided into two groups by half randomization and single blind method.The treatment group(N=65) received oral use of Yiyiren Gancao Decoction and washing the lesion with self-made Xiaoyou Decoction once a day except the microwave radiation therapy.The control group(N=63) received microwave radiation therapy alone.One month constituted one treatment course.The recurrence of RCA in the two groups was investigated within 3 treatment courses and within 6 months of follow-up.Results In the treatment groups,there were 37(56.92%) recurrent patients during the 1st treatment course,25(38.46%) during the 2nd course,and 11(16.92%) during the 3rd course;in the control group,there were 52(82.54%) recurrent patients during the 1st treatment course,47(74.60%) during the 2nd course,and 36(57.14%) during the 3rd course.The recurrence rate in the treatment group was lower than that in the control group(P

【Objective】To observe the effect of Yiyiren Gancao Tang (YGT) in preventing the recurrence of condylomaacuminatum (CA) after microwave therapy and to explore its influence on relative immunological indexes. 【Methods】Sixty-eight cases of CA were randomized into two groups: 34 cases (Group A) were treated with YGT and other 34 withplacebo decoction (Group B) after microwave therapy. The recurrence rate in the two groups were observed; seruminterleukin-2 (IL- 2) level and subtypes of T lymphocyte counting in peripheral blood were detected with double antibodysandwich immunoassay and rosette formation test before and after treatment.【Results】The recurrence rate was 20.5% inGroup A and 47.0% in Group B (P.05).【Conclusion】YGT can reduce the recurrence of CA, improve the immune function and is practical in clinics.

Adolescent ; Child ; Child, Preschool ; Cytokines ; blood ; Female ; Humans ; Interferon-gamma ; blood ; Interleukin-17 ; blood ; Interleukin-4 ; blood ; Interleukin-8 ; blood ; Male ; Purpura, Schoenlein-Henoch ; immunology

The methyl tertiary butyl ether(MTBE),as an addictive in gasoline,is used widely since the leaded gasoline has been prohibited.By some ways,MTBE can be transferred into water.Since the degradation of MTBE is very slow naturally,the pollution of MTBE in water is attracting more and more attentions.How to remove the MTBE economically and effectively has become a hot spot in common concern.In this paper,the species of photocatalytic degradation technique and the methods were reviewed including UV-Fenton,UV-H2O2,UV-O3,UV-TiO2 and visible-light photocatalytic decomposition with TiO2 were used to degradation of MTBE in water.

Objective To explore the CT and MR features of malignant peripheral nerve sheath tumor (MPNST) of adults.Methods The CT and MRI findings of 20 patients of MPNST confirmed by pathology were analyzed retrospectively.Results Among 20 cases,the lesions were located in the lower extremities (n=4),shoulder and spine (n=4),abdomen (n=3),chest wall (n=2),mediastinum (n=2),breast (n=1),prostate (n=1),scrotum (n=1),scalp (n=1),left maxillary sinus and orbital bottom (n=1).Nineteen cases appeared as solid masses and 1 case appeared as diffuse lesion.The maximum cross section of the lesions were about 1.41 cm× 1.42 cm-17.10 cm× 18.08 cm.Sixteen cases were ill defined margin and 4 cases were well-defined margin.CT scan showed 14 cases were lower density or isodensity and with patchy inhomogeneous density.Multiple calcification were found in 2 cases and osteolytic destruction were found in 7 cases.Enhanced CT of 10 cases showed solid component and gradually delayed enhancement,while cystic lesion and necrosis were not enhancement.The tortuous arteries were displayed in 7 cases.Seven cases were performed MRI and the lesions appeared as hypo-intensity on T1WI and hypo-intensity on T2WI with obviously high intensity of cystic component.Enhanced MRI of 3 cases showed significantly heterogeneous enhancement,cyst and necrosis had no enhancement.MR dynamic enhancement of 1 case showed time-signal intensity curve was platform type.DWI showed high signal.Conclusion MPNST has certain CT and MR characteristics which are helpful to improve the diagnostic accuracy.

Objective:To investigate the modulatory effect of IL-29 on trypsin-induced protease activated receptors (PARs) ex-pression on P815 mast cell.Methods:After P815 mast cells were challenged with different concentrations of IL-29 alone or combined with trypsin for 2 h, 6 h and 16 h, the challenged cells were collected and analysed by flow cytometry to detect the protein expression of PARs on P815 cells, and analysed by real time RT-PCR to detect the mRNA expression of PARs on P 815 cells.Results:Compared with the corresponding control , IL-29 induced significantly decreased expression of PAR-1 at protein and mRNA level on P815 cells, and upregulated PAR-3, PAR-4 mRNA level on P815 cells, whereas IL-29 did little effect on the expressions of PAR-2,3,4 at protein level on P815 cells accordingly.Preincubation of mast cell with IL-29 did not alter trypsin-induced PAR-1 expression on P815 cells, whereas up-regulated expression of PAR-2, 3, 4 were detected when P815 cell were pre-treated with IL-29 before being challenged with trypsin compared with the corresponding control .Conclusion: IL-29 can upregulate trypsin-induced PARs expression on mast cells through which participated in mast cell related inflammation .

Human osteoblast was treated with recombinant human connective tissue growth factor (rCTGF). This experiment showed that rCTGF increased membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 protein expression in a dose- and time-depentent manner in human osteoblasts. rCTGF induced activation of p38 MAPK in human osteoblasts. p38 MAPK inhibitor SB23058 abrogated the effect of rCTGF on the expressions of membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 in human osteoblasts.

Objective To investigate the effects of recombinant human connective tissue growth factor (rCTGF) on the proliferation, differentiation and apoptosis in human osteoblasts. Methods Human osteoblasts were treated with rCTGF, cell proliferation was measured by [3 H] -thymidine ([3 H] -TdR) incorporation method,the activity of alkaline phosphatase (ALP) was determined using α-nitrophenyl phosphate as a substrate, the expression of type Ⅰ collagen was determined by Western blotting, osteocalcin in conditioned media was measured by radioimmune assay, and cell apoptosis was assayed by flow cytometry. Results rCTGF promoted proliferation and differentiation of human osteoblasts in a dose-depentent manner, rCTGF increased ALP activity, osteocalcin and type I collagen secretion in a dose-depentent manner, rCTGF inhibited human osteoblastic apoptosis induced by serum deprivation. Conclusion rCTGF can promote osteoblastic proliferation, and differentiation and protect osteoblast against apoptosis, suggesting that CTGF plays an essential role in bone metabolism.

Objective To investigate the effects of recombinant human connective tissue growth factor (rCTGF) on the expression of osteoprotegerin (OPG) and RANKL ( receptor activator of NF-κB ligand ) in human osteoblasts, as well as the mechanisms involved. Methods Human osteoblasts were treated with rCTGF. The expressions of OPG and RANKL were assessed by Western blotting. The expressions of focal adhesion kinase ( FAK), mitogen-activated protein kinase (MAPK) were also observed. Results rCTGF inhibited RANKL protein expression in a dose-and time-depentent manner in human osteoblasts, while the expression of OPG kept unchanged. rCTGF induced activation of p38MAPK and dephosphorylation of FAK in human osteoblasts, but had no effect on ERK and JNK phosphorylation. p38MAPK inhibitor SB23058 abrogated the inhibitory effect of rCTGF on RANKL in human osteoblasts. Conclusion rCTGF inhibits the expression of RANKL in human osteoblasts via activation of p38MAPK and dephosphorylation of FAK.