Objective To observe the therapeutic effect of oral use and external application of Chinese herbal decoction for the treatment of recurrent condyloma acuminatum(RCA).Methods One hundred and twenty-eight RCA patients were divided into two groups by half randomization and single blind method.The treatment group(N=65) received oral use of Yiyiren Gancao Decoction and washing the lesion with self-made Xiaoyou Decoction once a day except the microwave radiation therapy.The control group(N=63) received microwave radiation therapy alone.One month constituted one treatment course.The recurrence of RCA in the two groups was investigated within 3 treatment courses and within 6 months of follow-up.Results In the treatment groups,there were 37(56.92%) recurrent patients during the 1st treatment course,25(38.46%) during the 2nd course,and 11(16.92%) during the 3rd course;in the control group,there were 52(82.54%) recurrent patients during the 1st treatment course,47(74.60%) during the 2nd course,and 36(57.14%) during the 3rd course.The recurrence rate in the treatment group was lower than that in the control group(P

【Objective】To observe the effect of Yiyiren Gancao Tang (YGT) in preventing the recurrence of condylomaacuminatum (CA) after microwave therapy and to explore its influence on relative immunological indexes. 【Methods】Sixty-eight cases of CA were randomized into two groups: 34 cases (Group A) were treated with YGT and other 34 withplacebo decoction (Group B) after microwave therapy. The recurrence rate in the two groups were observed; seruminterleukin-2 (IL- 2) level and subtypes of T lymphocyte counting in peripheral blood were detected with double antibodysandwich immunoassay and rosette formation test before and after treatment.【Results】The recurrence rate was 20.5% inGroup A and 47.0% in Group B (P.05).【Conclusion】YGT can reduce the recurrence of CA, improve the immune function and is practical in clinics.

Adolescent ; Child ; Child, Preschool ; Cytokines ; blood ; Female ; Humans ; Interferon-gamma ; blood ; Interleukin-17 ; blood ; Interleukin-4 ; blood ; Interleukin-8 ; blood ; Male ; Purpura, Schoenlein-Henoch ; immunology

The methyl tertiary butyl ether(MTBE),as an addictive in gasoline,is used widely since the leaded gasoline has been prohibited.By some ways,MTBE can be transferred into water.Since the degradation of MTBE is very slow naturally,the pollution of MTBE in water is attracting more and more attentions.How to remove the MTBE economically and effectively has become a hot spot in common concern.In this paper,the species of photocatalytic degradation technique and the methods were reviewed including UV-Fenton,UV-H2O2,UV-O3,UV-TiO2 and visible-light photocatalytic decomposition with TiO2 were used to degradation of MTBE in water.

Human osteoblast was treated with recombinant human connective tissue growth factor (rCTGF). This experiment showed that rCTGF increased membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 protein expression in a dose- and time-depentent manner in human osteoblasts. rCTGF induced activation of p38 MAPK in human osteoblasts. p38 MAPK inhibitor SB23058 abrogated the effect of rCTGF on the expressions of membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 in human osteoblasts.

Objective To investigate the effects of recombinant human connective tissue growth factor (rCTGF) on the proliferation, differentiation and apoptosis in human osteoblasts. Methods Human osteoblasts were treated with rCTGF, cell proliferation was measured by [3 H] -thymidine ([3 H] -TdR) incorporation method,the activity of alkaline phosphatase (ALP) was determined using α-nitrophenyl phosphate as a substrate, the expression of type Ⅰ collagen was determined by Western blotting, osteocalcin in conditioned media was measured by radioimmune assay, and cell apoptosis was assayed by flow cytometry. Results rCTGF promoted proliferation and differentiation of human osteoblasts in a dose-depentent manner, rCTGF increased ALP activity, osteocalcin and type I collagen secretion in a dose-depentent manner, rCTGF inhibited human osteoblastic apoptosis induced by serum deprivation. Conclusion rCTGF can promote osteoblastic proliferation, and differentiation and protect osteoblast against apoptosis, suggesting that CTGF plays an essential role in bone metabolism.

Objective To observe the clinical efficacy of heat-clearing, dampness-removing, and Qi-blood-tonifying Chinese herbal medicine compound for the treatment of recurrent genital herpes(RGH), and to investigate the expression levels of Th17 cells and Treg cells in peripheral blood of patients with RGH as well as their role in the pathogenesis of RGH. Methods Sixty RGH patients were randomly divided into experimental group and control group, 30 cases in each group. The experimental group was treated with Chinese medicine decoction, and the control group was treated with Aciclovir tablets orally. The treatment course covered 6 months. The percentages of Th17 cells and Treg cells in the peripheral blood of the RGH patients were detected with flow cytometry. Results (1) After treatment for 3, 6 months, the total effective rate of the experimental group was 53.33%, 80.00%, and that of the control group was 23.33%, 46.67% respectively. There were significant difference between the two groups(P < 0.05).(2) After treatment, the percentage of Th17 cells and the ratio ofTh17/Treg cells were decreased while the percentage of Treg cells was increased in the two groups, and the differences before and after treatment were significant in the experimental group(P<0.05) while were insignificant in the control group (P > 0.05). The inter-group comparison showed the decrease of Th17 cell percentage and Th17/Treg cell ratio as well as the increase of Treg cell percentage in the experimental group was superior to that in the control group, the differences being significant (P<0.05). Conclusion The curative effect of Chinese herbal compound for RGH is better than that of the control group. Chinese herbal medicine compound can decrease the level of Th17 cells, increase the level of Treg cells, thus to decrease the ratio of Th17/Treg cells, indicating that heat-clearing, dampness-removing, and Qi-blood-tonifying Chinese herbal medicine compound can correct the imbalance of Th17/Treg cells, and then plays a role in anti-inflammation and immune regulation.

Objective To investigate the effects of recombinant human connective tissue growth factor (rCTGF) on the expression of osteoprotegerin (OPG) and RANKL ( receptor activator of NF-κB ligand ) in human osteoblasts, as well as the mechanisms involved. Methods Human osteoblasts were treated with rCTGF. The expressions of OPG and RANKL were assessed by Western blotting. The expressions of focal adhesion kinase ( FAK), mitogen-activated protein kinase (MAPK) were also observed. Results rCTGF inhibited RANKL protein expression in a dose-and time-depentent manner in human osteoblasts, while the expression of OPG kept unchanged. rCTGF induced activation of p38MAPK and dephosphorylation of FAK in human osteoblasts, but had no effect on ERK and JNK phosphorylation. p38MAPK inhibitor SB23058 abrogated the inhibitory effect of rCTGF on RANKL in human osteoblasts. Conclusion rCTGF inhibits the expression of RANKL in human osteoblasts via activation of p38MAPK and dephosphorylation of FAK.

Objective:To investigate the significance of regulatory T cells in peripheral blood of chronic renal insufficientce.Methods:The peripheral blood samples were collected from 40 patients with chronic renal insufficient.The ratios ot CD4+T cell in lymphocyte and CD4+CD127-Treg and CD4+CD25+CD127-Treg in CD4+T were detected by flow cytometry.Results:The number of CD4+T in lymphocyte of chronic renal insufficient was higher than in healthy control group and there wasn’t significantly difference of the CD4+CD25+CD127-Treg ratios in CD4+T between chronic renal insufficience and healthy central.The ratio of CD4+T cells in lymphocytes of chronic renal insufficience was lower than in healthy control group except compensatory stage.There was no correlation between CD4+T cell ratios in lymphocytes,CD4+CD127-Treg or CD4+CD25+CD127-Treg ratios in CD4+T cells and the values of BUN,Cr among the hypertension patients.Conclusion:The number of CD4+T cells increases,and CD4+CD127-Treg decreases in the patients with chronic renal insufficience and their immune functions are shown in disoroler .