ObjectiveTo explore the characteristics and the pathogenetic mechanism of prospective memory in patients with chronic primary insomnia. Methods Forty-two patients with chronic primary insomnia and forty age,gender,education,and cultural level matched healthy adults were assessed with a battery of neuropsychological tests including event-based prospective memory (EBPM)and time-based prospective memory (TBPM) tasks.ResultsCompared with the healthy controls ( EBPM: 5.58 ± 0.35 ;TBPM: 4.10 ± 0.32),the patients with chronic primary insomnia had a significant loss in EBPM (3.44 ±0.40,t =3.933,P =0.000),but a insignificant impairment in TBPM (3.76 ± 0.29,t =0.787,P =0.433 ).The retrospective component of event-based prospective memory between the two groups had no significant difference ( t =1.308,P =0.195 ) ,neither did the retrospective component of time-based prospective memory ( Z =- 0.776,P =0.437 ).ConclusionThese results suggest that the patients with chronic primary insomnia have impairment in EBPM,but not in TBPM and the retrospective component of prospective memory.

A liver transplant invol ves more medical aspects than complex surgery,with different characteristics than general surgery.The medical risks the patient disputes focus on the informed consent rights.Analysis of dispute causes,especially through the relevant study of jurisprudence,suggests that we should be highly sophisticated with informed consent rights.to prevent disputes and improve the quality of clinical diagnosis and treatment.

AIM: To investigate the effects and mechanisms of atorvastatin on myocardial fibrosis induced by aldosterone in SD rats. METHODS: Forty male uninephrectomized SD rats were limited to drink 1% NaCl water for 4 weeks and assigned to the follow groups: vehicle control rats (CON group);aldosterone treated rats (ALD group);spironolactone + aldosterone treated rats(SPI+ALD group);atorvastatin + aldosterone treated rats (ATO+ALD group). Blood pressure was measured by catheterization. Expression of platelet-derived growth factor (PDGF-A, PDGF-B), platelet-derived growth factor receptor (PDGFR-α, PDGFR-β) and ectodermal dysplasia-1 (ED-1) were analyzed by immunohistochemistry. Collagen volume fraction (CVF) and perivascular collagen area (PVCA) were analyzed by Sirius-Red staining. Myocardium osteopontin protein was detected by Western blotting analysis. RESULTS: Mean arterial blood pressure in ALD group, SPI+ALD group and ATO+ALD group was elevated, and significant difference was observed between the three groups and vehicle control group (P<0.01 or P<0.05). Myocardial fibrosis was observed in ALD group. Compared to other three groups, the index of CVF and PVCA was increased significantly (P<0.01 or P<0.05). No significant difference of the index of CVF and PVCA between ATO+ALD group and SPI+ALD group was observed (P>0.05). Compared to other groups, the levels of PDGF-A, PDGF-B, PDGFR-α, ED-1 and OPN in ALD group were significantly increased (P<0.01 or P<0.05). The levels of PDGF-A, PDGF-B, PDGFR-α and OPN were no significant difference between ATO+ALD group and SPI+ALD group (P<0.01 or P<0.05). However, the level of ED-1 in ATO+ALD group was significantly decreased compared to SPI+ALD group (P<0.05). No significant difference of PDGFR-β level among four groups was found (P>0.05). CONCLUSION: These results suggest that atorvastatin may attenuate myocardial fibrosis induced by aldosterone. The mechanisms concern with reduction of macrophage infiltration, expression of inflammatory cytokines OPN, partially inhibition of platelet-derived growth factor and its receptor expression.

Objeetive To study the therapeutic effect of the H2 antagonist fomotidine on preventing weight gain olanzapine-induced.Methods Forty first-episode CCMD-3 schizophrenia patients were randomly allocated to received either famotidine or placebo in addition to olanzapine for 8 weeks.Their body weights and heights were measured before treatment and 2 weeks,4 weeks,8 weeks after treatment to calculate the body mass index and the percentage of patients gained more than 7% of initial bpdy weight after 8 weeks.SAPS and SANS were selected to evaluate the therapeutic effect.Results While the weight and the body mass index of patients in two groups after 2 weeks,4 weeks,8 weeks post-treatment increased significantly compared with those of pre-treatment(P＜0.05).The weight of patients in two groups gained 3.7kg at the edd of 8 weeks,and the BMI increased 2.5 or 2.6 kg/cm2,reapectively.However 8 weeks later,the difference between two groups wan not significant(P＞0.05).The difference between two groups on the percentage of patients gained more than 7% of initial body weight at the end of 8 weeks Wan not significant(P＞0.05).The sCOres of SAPS and SANS at the end of 8 weeks decreased significantly compared with those of the baseline(P＜0.05),but the difference between two groups was not significant(P＞0.05).Conclusion Allocated to the H2 antagonist fomotidine could not reduce weight gain olanzapine-induced.

Objective To design and implement the academic impact power information platform for the secondary disciplines of clinical medicine in order to keep abreast of their advantages and disadvantages. Methods An index system for the assessment of secondary disciplines of clinical medicine was constructed with the bibliometric methods according to the factual data. Results Its functions of query analysis, batch input, and editing of the index data of different disciplines were achieved in higher education institutions using the ASP. NET technology based on the data processing, indexing and statistical analysis. Conclusion The platform can provide the data support for the develop-ment and scientific research management of secondary disciplines, and thus playing an important role in promoting the innovative research and management of clinical medicine.

AIM:So far,endostatin is found playing the strongest effect to inhibit endothelial cells.Because endostatin protein is instable,it is difficult to prepare and apply.Gene therapy is used to assist endostatin to inhibit angiogenesis.This study constructed recombinant adenovirus of human endostatin gene using AdEasy-1 system to investigate the feasibility of expressing endostatin protein in ECV304 cells(human umbilical vein endothelial cells).METHODS:The experiment was performed at College of Life Science,Sun Yat-set University from March to October 2006.Endostatin was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector after sequencing and identification.The resultant plasmid was co-transduced into E.coli BJ 5183 cells with pAdEasy-1 plasmid for homologous recombination.The recombinant plasmid was detected by restriction analysis and DNA sequence analysis.The linearized recombinant plasmid was transfected into AAV 293 cells with LipofectAMINE2000.The recombinant adenovirus was detected by GFP,PCR and restriction analysis.The virus was used to infect ECV304,and the expression of endostatin in vitro was detected by the techniques of RT-PCR and Western blot.RESULTS:The positive clones of the recombinants were constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis.AAV293 cells were transfected.The titer of viral liquid was 2.06?1010 pfu/mL.The transcription of endostatin mRNA in ECV304 cells was checked by RT-PCR and endostatin protein could be detected in the culture by Western blot analysis.CONCLUSION:The recombinant adenoviral vector pAd-Endo carrying human endostatin is successfully constructed,and effective expression is found in ECV304 cells in vitro.

Medical nutrition therapy (MNT)is the basic and long-term treatment of diabetes patients. At present,the dietary structure of type 2 diabetes mellitus(T2DM)patients in China has yet to be further improved. This article focused on the effect,dietary structure,food selection and guarantee of compliance of MNT in T2DM.We recommended that the dietary pattern should be designed based on the traditional dietary habits around the country,combined with people living and working reality. The rational utilization of network platform can improve the efficiency and compliance of nutrition education.

In this paper, the theory of electrical character and adsorption of solidliquid interface was applied to study the preparation process for borneol-mi-croencapsule with co-coacervation using gelatin and arabic gum as materials. The optimum process conditions for preparing microencapsule was gained. This study method provided a reliable basis for raising the inclusion rate, decreasing the capsule materials in quantity and drawing up the process conditions.