Objective To develop and verify a high-performance liquid chromatography(HPLC) method for the determination of sucrose content in oral hexavalent reassortant live attenuated rotavirus vaccines(Vero cells), in order to provide a reliable method for the determination of sucrose content in vaccines with sucrose as an excipient. Methods A HPLC method for the determination of sucrose content in oral hexavalent reassortant live attenuated rotavirus vaccines(Vero cells) was developed. The chromatographic column was Luna?5 μm NH2100?(LC Colum 250 mm × 4. 6 mm), the differential detector temperature was 30 ℃, the mobile phase was acetonitrile, the flow rate was 0. 5 mL/min, the injection volume was 10 μL, and the column temperature was 30 ℃. The system suitability, specificity, linear range, precision, accuracy and robustness of the method were verified. At the same time, the consistency of the results of HPLC and polarimetry method for determining the sucrose content in the test sample was analyzed. Results The separation of lactose and sucrose in system suitability test solution was good, and the lactose did not interfere with the determination of sucrose content. The citric acid, sodium citrate, zinc chloride and calcium chloride showed no interference to the determination of sucrose content. The sucrose concentration at the range of 6-14 mg/mL showed a good linear relationship with the peak area, and the linear equation was: y = 142 189 x +2 182. 4, with the correlation coefficient(r) of 0. 999 9. The RSDs of reproducibility and intermediate precision verification were 0. 55% and 0. 49% respectively. The average recovery rates of samples with the concentration of 80%, 100%, and 120% were all between 99% and 101%. Minor changes in flow rate, column temperature, mobile phase ratio and detector temperature, or replacement of chromatographic columns of different batches had no effect on the test results, and the relative deviations(RDs) with the sucrose content measured under the original conditions were all less than 1%. The RDs of sucrose content in multiple batches of test samples determined by HPLC and polarimetry were all less than 1%. Conclusion The developed HPLC method has good specificity, precision, accuracy and robustness, and can be used for the determination of sucrose content in oral hexavalent reassortant live attenuated rotavirus vaccine(Vero cells).

Objective To explore the effect of individual nursing care on persistent headache in patients with intracranial aneurysm who accepted interventional embolization. Methods 100 patients with intracranial aneurysms were divided into observation group (n=50) and control group (n=50). The routine headache nursing care was taken in the control group and the individual nursing care was applied in the observation group. They were assessed with Visual Analogue Scale (VAS). Results The score of VAS reduced more in the observation group than in the control group (P<0.05), and the headache endurance and the satisfaction for nursing care were significantly better in the observation group than in the control group (P<0.05). Conclusion The individual nursing may relieve the pain, and improve the endurance of headache and the satisfaction for nursing care.

Aim To investigate the bio-affinities of ligustilide and butylidenephthalide to rat aortic smooth muscle cells and the inhibitory effects of them on bFGF-stimulated proliferation of rat vascular smooth muscle cell (VSMC). Methods VSMCs were cultured from rat aorta pectoralis and identified by an immunohistochemical method. The bio-affinities between solute (ligustilide or butylidenephthalide) and cell membrane were measured by rat aortic cell membrane chromatography (CMC). The inhibitory effects of ligustilide and butylidenephthalide on bFGF-stimulated VSMC proliferation were evaluated by MTT colorimetric method. Results Both ligustilide and butylidenephthalide had selective affinities to rat aortic smooth muscle cell as the same as verapamil, one of the calcium ion antagonists. They could potently separately (P ＜ 0.05 ), but had no effects on the normal VSMC growth. Conclusion Both ligustilide and butylidenephthalide can inhibit the abnormal proliferation of VSMC induced by bFGF.

Objective To establish a rate model of stable orthotopic liver transplantation using three-cuff technique.Methods 28 dead rats were dissected and the causes for death and the complication analyzed after liver transplantation using three-cuff method.Results The main postoperative complications included:bleeding,stenosis of liver superior vena cava,portal venous thrombus,respiration duct obstruction,gastrectasia,pneumothorax,adhesion of liver to diaphragm.Conclusion Most of 8 kinds of complications were related with operation.

Objective:To study the efficacy and safety of different types of quinolone antibiotics in the treatment of pelvic inflamma -tory diseases .Methods:Totally 70 patients with pelvic inflammatory diseases were selected and divided into the observation group and the control group according to the time of admission .The 35 patients in the observation group were treated with levofloxacin , and the con-trol group was treated with ciprofloxacin .After 20-day treatment, the clinical efficacy was observed and recorded in the two groups , and the adverse events were statistically analyzed .Results:The total effective rate of the observation group was 97.14%, which was signifi-cantly higher than that of the control group (82.86%, P<0.05).The recovery time of clinical symptoms and signs of the observation group was significantly shorter than that of the control group , and the difference was statistically significant (P<0.05).The difference in the incidence of adverse drug reactions was not statistically significant between the two groups (P>0.05).Conclusion:Levofloxacin in the treatment of patients with pelvic inflammatory diseates is with high clinical efficacy , which can rapidly improve the clinical symptoms and signs with high security .

Objective To evaluate therapeutic effects of portal azygous devascularization on patients with portal hypertension. Methods In this study, data of 212 patients with portal hypertension who underwent devascularization were analyzed retrospectively. Among them 52% patients had a history of variceal bleeding. Preoperative Child A accounted for 15% patients, B for 57% and C for 27%. Emergent operation and selective operation were performed in 11% and 89% cases, respectively. Hassab procedure was performed in 176 cases (83%), whereas modified Sugiura procedure in 36 cases (17%). Results Portal vein pressure decreased from (40?6) cm?H 2O to (36?7) cm?H 2O. The re-bleeding rate of 3, 5,10 years were 2%, 6%, 11%, and the survival rates of 3, 5, 10 years were 97%, 92% and 89%, respectively. Conclusions Devascularization is an effective procedure in controlling bleeding with low incidence of liver failure, operation mortality and encephalopathy. Emergent operation should be avoided as it causes high morbidity and mortality. The decreased portal pressure following devascularization may result in a reduced blood supply to the liver. Persistent exasperation of the liver function caused by chronic hepatitis and the development of hepatocellular carcinoma are the two major factors endangering long-term survival.

Objective To explore the effect RNAi-mediated inhibition of X-linked inhibit apoptosis protein (XIAP) gene on apoptosis and susceptibility to chemical therapy drugs in osteosarcoma cells and its underlying mechanism.Methods shRNA plasmid of XIAP gene were constructed. MG63 cells were conventionally cultured and divided into untreated group (group A,non-transfection),blank plasmid group (group B,psiRNA-Con transfected),the experimental group (group C,psiRNA-XIAP transfected),and 2 chemical drug interfered groups,non-transfection+0.3 ?mol Dox group (A+Dox group) and psiRNA-XIAP transfection +0.3 ?mol Dox group (C+Dox group). The XIAP expression in MG63 cells was detected by Western blotting. The proliferation of above-mentioned MG63 cells was examined by MTT assay. Flow cytometry (FCM) was employed to detect the cell apoptosis,cell cycle and Caspase-9 expression. Results Group C had a significantly lower protein level of XIAP and MTT value than group A and B (P

In this article 108 human fetuses from 12 to 30 weeks of gestation, 3 neonates and a 1.5 years child were used for study of the histogenesis of the appendix.In 12 weeks old fetus, the appendix showed small lumen and thin wall. The mucosa presented intestinal villi and crypts. The endocrine and goblet cells could be found. The circular muscle and serous membrane had differentiated. In the specimens of 13 to 15 weeks, the lamina propria contained diffused lymphatic tissue and lymphatic follicles. On such sites, the intestinal villi were absent. The surface epithelium became attenuate and composed of cuboid or lower cuboid cells. The outer longitudinal muscle layer was evident. The scattered muscularis mucosa was found. At 16 weeks, the endocrine cells appeared and showed various forms. The open-type or closed-type endocrine cells could be distinguished easily. Under EM, based on the morphology of the basal granules, the endocrine cells might be divided into two types. The granules were cell enclosed in a limiting membrane. One type of the granules was round and various in size, and another type was irregular. In the surface epithelium covered on the lymphatic tissue, the microfold cells could be found. They showed irregular microvilli or mierofolds and numerous apical micropinocytotic vesicles. At 18.5 weeks, the villi tended to merge each other. At 20 weeks, the muscularis mucosa was formed and the 4 layers of appendix resembled those of the normal adult. We found endocrine cells aggregated into Segi's cap on the apex of the villi in 7 fetuses of 16-21 weeks. The T or B lymphocytes could be identified as early as 13.5 weeks by immunologic method. We found the percentage of the B lymphocytes decreased with the gestation age, and this fact suggested that the appendix might be a B lymphocyte pool during the fetal period.

The coming of an aged society in China will surely greatly affect the chief concerns of health care. The Chinese orthopaedists should be prepared for the tasks imposed by the new situation. Once a fracture happens due to osteoporosis in a senile person, a series of problems will follow. This paper discusses the diagnosis and prevention of osteoporosis, preoperative assessment of the patient, proper application of internal fixation and combined treatment of fracture and osteoporosis. The critical point is how to improve the therapeutic effects and to reduce the operative risks.

Animals ; Dose-Response Relationship, Drug ; Humans ; Inhalation Exposure ; Mice ; Nanoparticles ; toxicity ; Rats ; Siphonaptera