Multiple tumor marker protein chip(C-12) quantitatively measures the level of twelve tumor marker proteins in human serum, therefore can assist the diagnosis of liver cancer, stomach cancer, colorectal cancer, pancreatic cancer and bile duct cancer. Compared with current diagnostic methods, C-12 is less time consuming, requires smaller samples, and still provides a wider detecting range and a higher accuray. C-12plays an important role in clinical dignosis of digestive system tumors.

Objective To analyze the results of gold labeled immunochromatographic assay (GICA ) ,enzyme-linked immunosor-bent assay (ELISA ) and time resolved fluoroimmunoassay (TRFIA ) detection for hepatitis B virus surface antigen (HBsAg ) . Methods GICA ,ELISA and TRFIA were employed to conduct the HBsAg testing in 11 058 specimens .Results The HBsAg posi-tive rates of GICA ,ELISA and TRFIA detection in 11 ,058 specimens were 15 .52% ,15 .64% and 15 .95% ,respectively ,with no statistically significant difference when pairwise comparison performed (P>0 .05) .In specimens which results of TRFIA quantita-tive detection was 0 .2- <2 .0 ng/mL ,the diagnostic compliance rates of GICA ,ELISA were 25 .0% and 75 .0% ,respectively .Con-clusion The diagnostic compliance rates of GICA ,ELISA detection in specimens with low concentration HBsAg are deceased .

OBJECTIVE:To study the pharmacokinetics of aclacinomycin A polylactide lyophilized nanopartical (ACM-A-PLA-NP) in plasma of rabbit.METHODS:HPLC was selected to determine the concentration of aclacinomycin A in plasma after a single dose of ACM-A-PLA-NP and lyophilized ACM-A.RESULTS:The pharmacokinetic parameters of these two dosage forms were obtained by 3p87.CONCLUSION:The lyophilized ACM-A-PLA-NP has a more sustained release character comparing with the lyophilized ACM-A.

It has been confirmed that tumor invasion and metastasis is not only dependent on the charac -teristics of the tumor ,but also dependent on the tumor microenvironment .Tumor-associated macrophage ( TAM) , which constitutes a large number of the tumor -infiltrating immune cells,is stimulated into M1 macrophage and M2 macrophage by different signals in the tumor microenvironment .Whereas the vast majority of studies with nu-merous tumor types show that the presence of M 2 phenotypes in the tumor microenvironment is associated with a worse prognosis ,most studies in gastric cancer also claim the same results .Hypoxia and coagulation factors may both contribute to it .It has identified that the function of TAM could be changed with their location of tumor ,but it has nothing to do with cell differentiation and cell type of gastric cancer .In this review,we summarize the differ-ent and controversial effects of TAM in gastric cancer .

OBJECTIVE: To study the equilibrium solubility of aesculin and its apparent oil/water partition coefficient,and to provide basis for absorption mechanism study.METHODS: Equilibrium solubility of aesculin at the pH of 1.2～8.0 was determined by saturation method.The apparent oil/water partition coefficients of aesculin were determined by shaking flask method.RESULTS: The equilibrium solubility of aesculin at the pH of 1.2～6.8 was fluctuated slightly;it increased rapidly at the pH of 7.4;the maximum equilibrium solubility of aesculin was obtained at the pH of 8.0(10.58 g?L-1).The oil/water partition coefficient of aesculin changed slowly at the pH of 1.2～6.0;it decreased significantly at the pH of 6.0;the minimum oil/water partition coefficient was obtained at the pH of 8.0(0.03).CONCLUSIONS: The equilibrium solubility of aesculin and its apparent oil/water partition coefficient are associated with the pH of medium.The reason that aesculin has no effect on uric acid after oral administration is relevant to poor gastrointestinal tract absorption ability.

Five resin casts of the coronary arteries and the hislological sections of three hesrts with the arteries injected Chinese ink were prepared in human adult autopsy subjec s . 3187 branches of the coronary arterial trees were counted and measured . By Strahler's system the branch pattern of the coronary arterial tree was described . According to the analysis of the geometrical morphology data, the relationships between the diameter , the length and the number of branches in each order and the branch order show the exponential function respectively , which have high correlation significance (p

0.05). The ITA contains relatively higher content of elastin as compared with the others (P

OBJECTIVE: To investigate the clinical features of primary biliary cirrhosis (PBC) and its treatment by integrated traditional Chinese and western medicine. METHODS: 16 PBC patients were observed. Ursodeoxycholic acid (UDCA) was used in the dose of 13 to 15 mg.kg(-1).d(-1), with some traditional Chinese herb prescription. Results (1) The proportion of women to men was 15:1, the mean age was 52.5 years. AMA-M2 was positive in 14 subjects (87.5%). Biliary tract enzymes and ESR were elevated in all subjects. The ratio of hypercholesterolemia (CHOL) and abnormality in IgM was high (62.5%). Fatigue, pruritus, arthralgia, jaundice, splenomegaly were noted in more than half cases. Every patient had one to four complications. (2) ALP, GGT and Glb declined evidently after 3 months' treatment by western medicine associated with traditional Chinese medicine, and declined markedly after 12 months' treatment (P<0.05 respectively). TBIL and CHOL declined gradually during the treatment course. Symptoms and signs were lightened. CONCLUSION: PBC has complicated and especial clinical features. UDCA therapy is effective in PBC, while traditional Chinese medicine has extraordinary effect in treating the symptoms and signs.

Objective To explore the application effect of clinical nursing pathway on maintenance hemodialysis patients.Methods Totally 120 patients with maintenance hemodialysis between January 1 and December 31 2009 were randomly assigned to the control group and the observation group with 60 patients in each group.The clinical pathway was established and applied to the observation group.The treatment effect was compared between two groups.Results The incidence of complications and nursing errors declined with the application of clinical nursing pathway,and the doctor's orders compliance and the satisfaction and quality of care were improved significantly.Conelusions The application of clinical nursing pathway in maintenance hemodialysis patients is an effective method for improving overall care quality and the satisfaction degree of the patients with nursing,so as to improve their survival rate.

Computer science is not important enough in the medical university curriculum and students do not like this course. Therefore, game-teaching methods were employed to enrich teaching methods,to make medical teaching more vivid and to promote medical students' learning interests. In this paper,application of game-learning idea in the medical teaching process was discussed from multiple aspects including the story of the game,incentives of the game and the punitive measures of the game.