Objective To compare the efficacy of acrossing the injured vertebra percutaneous pedicle screw fixation and traditional open surgery for Magerl A thoracolumbar fractures.Methods Retrospective analysis 56 patients with single segmental Magerl A thoracolumbar fractures without neurological symptoms were stabilized,including percutaneous fixation in 26 cases and open fixation in 30 cases.The operation time,intraoperative,blood loss,incision length,sagittal Cobb angle,height of injured vertebral,VAS,ODI,were compared between 2 groups.Results The precutaneous group less surgical blood loss,incision length and postoperative go to the fields time than the open group (P ＜ 0.05).There were improvements in Cobb angle and anterior height in two groups (P ＜ 0.05).The precutaneous group had lower VAS and ODI scores postoperation (P ＜ 0.05).The fracture vertebral front height and Cobb angle had significantly loss at final follow up than on the third day postoperation (P ＜ 0.05).Conclusions The percutaneous pedicle screw fixation can achieve similar effect in the treatment of Magerl A thoracolumbar fractures without neurological symptoms than open surgery,with less iatrogenic injury,less intraoperative blood loss and quick recovery for patients.

In this study, a spectrophotometric detection method for diethylstilbestrol (DES) was proposed by reducing silver nitrate (AgNO3) to obtain silver nanoparticles (AgNPs) in the medium of ammonia and sodium hydroxide. It was found that the resulting AgNPs have plasmon resonance absorption (PRA) characteristic at 415 nm, and the PRA is proportional to the increase of DES concentration in the range of 4.0×10-8-1.0×10-5M with the detection limit (3σ) of 1.2×10-7M. Most of the coexisting substances at high concentrations did not affect the detection of real samples, such as tablets. The recovery was in the range of 96.01%-107.41% and the RSD was lower than 4.7%. This method can be successfully applied to control preparation quality of DES.

Objective To analyze the different procedures and their clinical effects on treatment for pan-creatic pseudocyst( PPC) .Methods The management and outcome of 78 patients with PPC from Jan.2002 to Jun.2012 were retrospectively analyzed.Results 20 patients underwent conservative treatment and the pseudo-cysts absorbed spontaneously during follow up.Invasive procedures were performed in 58 cases, including percu-taneous catheter drainage in 3 cases with 1 postoperative pancreatic leakage and 1 postoperative pancreatic ab-scess;PPC external drainage in 5 cases with 2 postoperative pancreatic leakage and 1 postoperative bleeding;pancreatic pseudocyst stomach anastomosis in 15 cases with 1 postoperative gastrointestinal bleeding;Roux-en-Y cystjejunostomy in 29 cases with 2 gastrointestinal bleeding;cystduodenostomy in 2 cases;pancreatic pseudocyst excision in 2 cases with 1 postoperative pancreatic leakage; and laparoscopic cystogastrostomy in 2 cases.Con-clusions At present, there are various treatment options for pancreatic pseudocyst and it is necessary to choose treatment strategy according to patient's condition.Internal drainage is still the primary option of surgical proce-dures in treatment for pancreatic pseudocyst.

Giving courses of "Tea Nutrition and Health" in Guangdong Pharmaceutical University,popularizing tea culture will play a role in promoting Guangdong tea industry and tea economics,while tea culture will also have an important impact on social harmony,national unity. In this paper,the author discusses the condition of students'elective course,course content,teaching reform and innovation,problems and improvement measures,etc. to make a few practices and experiences.

Objective To explore the reliability, advantages and precautions of trans-umbilical single site double-ports laparoscopy hernia sac high ligation in children. Methods Retrospective analysis of the clinical data of 102 patients of trans-umbilical single site double-ports laparoscopy hernia sac high ligation from April 2015 to June 2016, then summarize the treatment process, method and experience. Results All 102 cases of children with inguinal hernia, 84 cases of unilateral, bilateral in 18 cases. Unilateral cases, found in 24 cases of lateral occult hernia. Operation time: one side (16.17 ± 8.23) min, bilateral (23.68 ± 9.72) min. All the patients were discharged after 1 d, and the incision was beautiful. There was no swelling in the scrotum or labia, no hematoma, no adhesive intestinal obstruction, and followed up from 6~20 months without recurrence. Conclusion It is safe, reliable and effective to treat inguinal hernia in children with trans-umbilical single site double-ports laparoscopy hernia sac high ligation. It has advantages such as beautiful incision, small trauma, quick recovery, low requirements on technology and equipment, easy to grasp.

Objective: The experiment was designed to study the effects of compound Konjac glucomannan on hyperlipidemia for the purpose of improving the therapeutic efficacy. Method: First, hyperlipidemia rat models were established. Then the effects of compound Konjac glucomannan and Konjac glucomannan on blood lipids levels and hepatic tissue morphous of rats were detected. Result; The compound Konjac glucomannan could decrease LDL-C and ApoB levels of serum TC significantly, while notably increase HDL-C and ApoA-1 levels. The result also shows that compound Konjac glucomannan could obviously improve the TG metabolism and decrease blood glucose concentration. The morphologic observation indicates that both Konjac glucomannan and flavonids could protect liver tissues of rats. Conclusion; Compound Konjac glucomannan could improve regulative ability of lipids metabolism and decrease serum lipids levels.

Bacillus anthracis , the aetiological agent of anthrax, was the first discovered pathogenic bacterium in history Rapid progresses have been made on this field in recent years, especially; Bacillus anthracis has been sequenced successesfully early this year and published on the Internet The anthrax pathogenesis is always central to the study and there has been an enormous amount of work to elucidate it In this review, we will focus on the latest findings that concern three aspects of anthrax pathogenesis: Bacillus anthracis genome, pathogenic substances and pathogenesis mechanism

Heavy chain antibodies (HcAb) without light chains are naturally produced by camelids.The single domain antigen-binding fragment of HcAb is referred to as VHH or nanobody (Nb),which is the smallest antigen-binding entity at present.Several characteristics such as low molecular weight,high stability,and low immunogenicity make the use of nanobodies superior to the conventional antibodies.Currently,nanobodies are highly valuable antibodies for various applications,including fundamental research,diagnostics,and therapeutics.A variety of nanobodies are investigated in clinical researches.The recent progresses of heterologous expression of nanobodies in seven expression systems,including Gram-negative and positive bacteria,yeasts,filamentous fungi,insect cells,mammalian cells and plant cells were focused on,and the expression systems,hosts,characteristics of vectors,construction of vectors and yield of nanobodies were summarized.The strategies to increase the yield of nanobodies are discussed from molecular level,expression level and rational design.

Objective To investigate the change of regulatory T-cells (Treg) before and after therapy in patients with autoimmune hemolytic anemia (AIHA),and to study the role of Treg in AIHA.Methods Treg cells numbers was measured by flow cytometry.Results Before treatment,Treg cells in AIHA patients was (1.32 ± 0.87) ％,which was significantly lower than (3.08 ± 0.96) ％ in the controls (t =-5.37,P ＜ 0.01).After treatment,Treg cells in AIHA patients was significantly increased [(4.96 ± 1.13)％] (t =-16.94,P ＜0.01).Conclusion Treg cells decreased in AIHA patients.Glucocorticoid might play a role in AIHA treatment by up-regulating Treg cells number.

Objective To investigate the relationship between sarcoplasmic reticulum Ca2+modulation proteins and postresuscitation myocardial dysfunction. Methods Thirty-eight SPF male Sprague-Dawley (SD) rats were randomly divided into control group(n=12)and cardiac arrest(CA)group(n=26). CA was induced by intravenous bolus of potassium chloride(40μg/g),and cardiopulmonary resuscitation(CPR)was conducted 8 minutes later. No CA was induced in control group except catheter placement for monitoring cardiopulmonary parameters after anesthesia. Invasive hemodynamic parameters were monitored for 1 hour after CPR. Echocardiogram was performed to evaluate cardiac function. Myocardial samples were harvested 5 minutes and 1 hour after restoration of spontaneous circulation (ROSC),and sarcoplasmic reticulum Ca2+ ATPase (SERCA2a),phosphorylated phospholamban (p-PLB) and rynodine receptor(RyR)were determined by Western Blot. Results ROSC rate of CA group was 92.3%(24/26),and mean recovery time was (68 ±39)seconds. Cardiac function was significantly impaired in CA group at 1 hour after resuscitation, and ejection fraction, fraction shortening (FS), the maximal rate of left ventricular pressure increase/decline (±dp/dt max)were significantly decreased compared with those in control group 〔ejection fraction:0.548±0.060 vs. 0.809±0.043,F=71.692,P=0.000;FS:(34.4±4.4)%vs. (46.0±3.5)%,F=55.443,P=0.000;+dp/dt max(mmHg/s):4 718±743 vs. 7 098±394,P<0.01;-dp/dt max(mmHg/s):-3 824±612 vs.-6 187±473,P<0.01〕. Compared with control group,the expression levels of p-PLB (gray value)was significantly decreased at 5 minutes and 60 minutes(5 minutes:0.64±0.15 vs. 1.29±0.13,P<0.01;60 minutes:0.95±0.08 vs. 1.30±0.09,P<0.05)after resuscitation in CA group,while the level of sarcoplasmic SERCA2a(gray value)and RyR (gray value)showed no significant differences(SERCA2a 5 minutes:1.01±0.18 vs. 1.24±0.07,60 minutes:1.03± 0.14 vs. 1.25 ±0.06;RyR 5 minutes:0.96 ±0.13 vs. 0.97 ±0.13,60 minutes:0.88 ±0.14 vs. 0.99 ±0.11,all P>0.05). Conclusions The impairment of the p-PLB is closely related to postresuscitation myocardial dysfunction.