Objective To investigate effect of dicoumarol on the expression of serum tumor necrosis factor-α( TNF-α) in patients with acute cerebral infarction.Methods Eighty cases patients with acute cerebral infarction were admitted and randomly divided into two groups by digital draw:control group and observation group, 40 cases in each group.The control group were given conventional therapy for treatment of acute cerebral infarction, and observation group were given dicoumarol thrombolysis on the basis of control group.The serum TNF-αlevel and neurologic impairment score were compared between two groups pre-and post-treatment.Results After treatment of 6 h, the serum TNF-αlevel in observation group was significantly lower than that in control group (P<0.05).After treatment of 24 h, 48 h and 30 d, there were no significant differences of serum TNF-αlevels between two groups.After treatment of 5 d, 14 d and 28 d, the neurological deficit scores in observation group were significantly higher than those in control group (P<0.05).Conclusion Dicoumarol could effectively reduce TNF-αlevels in serum and extent of cerebral cell damage in patients.

The cerebral GABA-T activity of mice was determined by the modified coupled-enzyme spectroflourimctry, and the method was proved stable and feasible. The cerebral GABA degradation of mice in vitro was inhibited by the sera from the mice with hepatic coma induced by galactosamine. The supernatant of liver homogenate of both control and hepatic coma groups can inhibit the cerebral GABA degradation too. The inhibitory capacity is proportional to the volume of LHS added in certain conditions, and reaches a maximum inhibitory rate of 80%. The inhibition can not be relieved by pyridoxal phosphate.The inhibitory effector of cerebral GABA degradation has been regarded as liver specific or mainly presented in the liver since no appreciable inhibition was found after the addition of the supernaiants of kidney, heart, lung and spleen homogenates. The possible contribution of GABA degradation inhibition by LHS to the pathogenesis of hepatic encephalopathy has also been discussed

Objective:To explore the effect of intraventricular administration of insulin on pro-apoptotic expression of Caspase-3 mRNA and neuronal apoptosis in hippocampus CA1, and neurological function after rats’ CPR. Methods:Thirty male SD rats were randomly divided into three groups:sham group;the resuscitation with saline-treated group and resuscitation with insulin-treated group. Six minute cardiac arrest was induced by ventricular fibrillation (VF) via pacing electrode placed in rats’ esophagus in saline and insulin group. Results:1. The NDS revealed a clear neurological deficit after reperfusion 24 h, 72 h in insulin and saline group as compare to the sham group, Comparing to the saline group, Insulin could improve the rats’ neurologic function after CPR 24 (insulin vs. saline group, 70(64~72)vs.56(50~58), P<0.001);2. Through TUNEL stain, insulin inhibited apoptosis in CA1 hippocampus as compare to the saline after CPR 7d. (F=5.853, P=0.02) 3.Caspase-3 expression in insulin-treated groups were significantly decreased compared to the saline-treated group after reperfusion 24h and 72h[(70(64~72), 56(50~58);P<0.001]. Conclusion:Intraventricular administration of insulin could inhibit the mRNA expression levels of the apoptotic activities of Caspase-3 after cardiac arrest, prevent neuronal apoptosis in the CA1 hippocampus and improve the rats’ neurological function (at least 24hours) after CPR.

Objectives: To investigate the characteristics and their functional significance of TMJ disc and its attachment. Methods: Each of the 23 TMJs from 12 cadavers were divided into quadrants sagitall y, and total 92 histological slices were obtained and stained with HE.The morpho logical characteristics of the samples was observed microscopically. Results: ①Both temporal posterior and anterior mandibualr attachment s were loose while mandibular posterior and anterior temporal attachments were d ense in which the fibers run anterior posteriorly. ②Fibers in the intermediate zone ran predominantly in anterior posterior direction while those in both ant erior and posterior bands were mixed with transverse fibers. ③At the posterior portion of disc there were 33 out of 92 slices from 6/12 cadavers (11/23 TMJs) s howed the structure of "the fourth band" which was featured by dense fibers alik e those of inner disc. There were 36 out of 92 slices from 6/12 cadavers (10/23 TMJs) showed the structure of "turned fibers" which was featured by a sudden tu rning of fibers from the thick posterior band back and downward, providing an angle like sign. Conclusions: There is great difference o f fiber direction in various parts of disc and its attachments, reflecting the functional demands.

OBJECTIVE:To study the mechanisms of GJKL(Gujikang liniment) in treatment of soft tissue injury METHO_DS:The effects of intraperitoneal injection of acetic acid solution on capillary permeability were observed;An animal model of soft tissue injury was established by striking The effects of GJKL on hemorheological parameters were observed RESULTS:GJKL markedly inhibited capillary permeability and decreased hemorheological parameters CONCLUSION:GJKL takes therapeutic effects on soft tissue injury through inhibiting the increased capillary permeability and reducing the hemorheological parameters

Objective To uncover the possible factors of rapid increasing incidence of syphilis. Methods The clinical data of 100 patients firstly diagnosed as syphilis and their spouses were analyzed. Results There were 65 couples (130 cases) both were infected with syphilis, accounting for 65%. Among these cases, 25 males firstly diagnosed, 15 of them were primary syphilis; and 40 females firstly diagnosed as syphilis, 32 of them were secondary syphilis. Among 65 syphilis couples, 48 spouses were quickly diagnosed as syphilis through contact tracing, but the other 17 spouses were not diagnosed as syphilis until 1~3 months later, and most of them were latent syphilis, accounting for 83.2%(54/65). Chancre, which may be noticed by the patients themselves, was not so common in this group of patients, accounted for only 28.5%(37/130), and mainly in males. Conclusions The infections rate of syphilis between spoused is high, especially latent syphilis, and chancre is low, which might be one of the factors for the rapid spreading of the disease.

Mast cells (MCs) play a key role in the pat hogenesis of allergic diseases. Tissue MCs are originated from hematopoietic ste m cells in bone marrow. In recent years, it was reported that human mast cells c ould be differentiated from stem cells of umbilical cord blood. In this review, we summarize the development in this novel area.

AIM: To investigate the actions of trypsi n on the secretion of monocyte chemoattractant protein-1 (MCP-1) from human lung e pithelial cells. METHODS: A549 cells were cultured in a 12-well culture plate. Th e challenge was performed by addition of various concentrations of trypsin or tr ypsin inhibitor into each well, respectively. After 2 h, 8 h or 16 h, the reacti ons were terminated by removal of the supernatant from each well. A sandwich ELI SA was used to determine the levels of MCP-1 in supernatants. RESULTS: Following 16 h incubation, trypsin was able to induce c oncentration-dependent secretion of MCP-1. As low as 3 ?g/L trypsin was able to induce MCP-1 release from epithelial cells, and the maximum of accumulated rele ase of MCP-1 was observed with 100 ?g/L trypsin, which was 3 fold more than bas eline release. However, trypsin at 300 ?g/L did not induce significant MCP-1 se cretion. Soybean trypsin inhibitor (SBTI) inhibited trypsin-induced MCP-1 secret ion, but ? 1-antitrysin (? 1-AT) did not. The time course showed that the actions of trypsin initiated at 2 h and reached their peak at 16 h. CONCLUSION: Trypsin is a potent secretogogue of MCP-1 release fr om cultured human lung epithelial cells, and itself action can be inhibited by S BTI.

OBJECTIVE:To compare the dissolution rates of levofloxacin tablets produced in 4 domestic pharmaceutical manufacturers in order to provide the evidence for its clinical use.METHODS:UV spectrohpotmetry was used to determine the cumulative dissolution rates of these 4 kinds of levofloxacin tablets at different time points,the dissolution parameters of T50,Td,T80,and M were calculated by Weibull formula,then variance analysis was made.RESULTS:The dissolution rates of these 4 kinds of levofloxacin tablets all met the standards recorded in 2000 edition of China Pharmacopoeia,but significant differences were found in parameters of T50,Td,T80,and M(P