Background: Lack of health education could potentially become a crucial risk factor in preventing Human Immunodeficiency Virus (HIV)/Acquired Immunodeficiency Syndrome (AIDS). Globally it does not have any cure, and no effective vaccines are available. The study aimed to assess the effect of the maieutic sensitization Program on the Level of Knowledge Regarding HIV/AIDS and People Living with HIV (PLHIV) among Health Care Students (HCS) in India. Materials and Methods: A quantitative study with an evaluative approach of randomized controlled trial design with study group and control group of 600 students from first-year Bachelor of Dental Science (BDS) and Basic B.Sc. Nursing. HIV knowledge questionnaire 30 explored the level of knowledge regarding HIV/AIDS and PLHIV with the probability multistage cluster random sampling technique. The collected data were analysed using SPSS. Results: Comparing the level of knowledge of 600 health care students between the study and control groups during the pre-test had no statistically significant difference at p=0.62 level. In post-test I and II, the number of students who had adequate knowledge in the study group was more than that in the control group (51% vs. 34%) and (83.6% vs. 57%), respectively. Conclusion Based on the present study's statistical evidence, the Maieutic Sensitization Program (MSP) was influential on HIV/AIDS and PLHIV among health care students in the study group than the control group who did not receive the study MSP.