1.Parents'marital relationship and intergenerational transmission of value orientation of marriage and love in college students
Huijie TONG ; Zhenlin DU ; Haorui ZHAO
Chinese Mental Health Journal 2015;(9):714-720
Objective:To investigate relationship between parents'marital relationship and intergenerational transmission of value orientation of marriage and love among college students.Methods:Totally 712 families inclu-ding a college student and his/her parents from 9 universities were surveyed.The Marital Health Test (MHT)was used to assess parents'marital relationship and transmission of value orientation of marriage and love from father and mother.The structural equation modeling and stepwise multiple regression were used to analysis the relationship between parents'marital relationship and intergenerational transmission of value orientation of marriage and love a-mong college students.Results:Moderate correlations (r =0.24 -0.58,Ps <0.01 )were found between parents'marital relationship and transmission of value orientation of marriage and love from father and mother.Path analysis suggested that paths from parents'marital relationship to paternal transmission and maternal transmission were statis-tically significant (path coefficient was 0.59 and 0.75).Grouping regression analysis showed that the influences of parents 'marital relationship of same-sex transmission (R2 =0.49,0.35 ) were greater than opposite-sex transmission.Conclusions:The findings indicate that parents'marital relationship could facilitate the intergeneration-al transmission of value orientation of marriage and love.Moreover,the influences of parents'marital relationship of same-gender transmission may be greater than opposite-sex transmission.
2.Correlation Between State-trait Anxiety and Sleep Structure in Patients with Insomnia
Haorui DU ; Hongzu ZHAO ; Hongli YAN ; Yufeng TANG ; Erlong CUI ; Chong LI
Chinese Mental Health Journal 2009;23(12):868-871
Objective:To explore the relationship between sleep structure changes and state or trait anxiety in patients with insomnia.Methods:Investigations were conducted with the State-trait Anxiety Inventory (STAI) and whole-night ploysomnography (PSG) recorded in 31 patients with insomnia and 20 normal controls.Repeated examinations were conducted in return visits 3~4 months after discharge.Results:Compared to control group,the patients with insomnia had decreased sleep time[(333.71±84.33) min vs.(403.65±19.29) min] and sleep efficiency[(70.41±17.35) % vs.(83.45±4.42) %],and increased sleep latency[(39.48±24.24) min vs.(19.65±8.57) min],REM sleep latency[(106.60±42.89) min vs.(86.80±12.25) min],proportion of S_1 sleep time[(25.36±14.22) % vs.(8.86±1.77) %]and awakening times[(4.45±2.51)vs.(1.75±1.07)].The proportions of S_(3+4) time[(7.38±9.70) % vs.(13.78±4.24) %] and REM sleep time reduced[(14.54±5.61) % vs.(19.18±2.14)%] (Ps<0.05).According to the STAI,scores of both state anxiety[(47.94±8.96) vs.(39.15±4.51)] and trait anxiety[(49.94±8.90) vs.(42.05±7.13)] were significantly higher in insomnia group than in control group (Ps<0.05).State anxiety was positively correlated with sleep latency (r=0.42),REM sleep latency (r=0.25),awakening times (r=0.44) and proportion of S_1 sleep time (r=0.34) (Ps<0.05),and negatively correlated with proportion of REM sleep time (r=-0.41,P<0.01).Trait anxiety was positively correlated with sleep latency (r=0.37,P<0.01) and wakefulness frequency (r=0.29,P<0.05).In return visits,the sleep structure improved,state anxiety score reduced,and trait anxiety had no significant changes.Conclusion:Patients with insomnia have significant sleep structural changes and higher state and trait anxiety.Their sleep structural changes are possilly related to state and trait anxiety.
3.Development and application of real-time RT-PCR and S1 protein-based indirect ELISA for porcine deltacoronavirus.
Jingwei WANG ; Ximei LEI ; Pan QIN ; Pengwei ZHAO ; Bin WANG ; Yiwen WANG ; Yiting LI ; Haorui JIN ; Long LI ; Yao-Wei HUANG
Chinese Journal of Biotechnology 2017;33(8):1265-1275
Porcine deltacoronavirus (PDCoV) has been recently recognized as an emerging viral pathogen that causes diarrhea in newborn piglets. A total of 254 small intestinal or fecal samples collected from 10 provinces including Henan, Hunan, Zhejiang, Jiangxi, Anhui, Hebei, Heilongjiang, Jiangsu, Shandong and Shanghai between 2014 and 2015, were screened by quantitative RT-PCR targeting the viral M gene. Eleven PDCoV positive samples were identified with a total positive rate of 4.33%. An indirect enzyme-linked immunosorbent assay (ELISA) was developed based on the recombinant S1 protein of PDCoV. This assay was used to test 609 serum samples of pigs with diarrhea symptoms collected from 10 provinces between 2015 and 2016. The positive rate of PDCoV antibody was 44.17% (269/609). The two methods can be used to monitor the PDCoV epidemiology in the levels of PDCoV specific RNA or antibody, helping better prevent and control PDCoV.