ObjectiveTo evaluate the efficacy and feasibility of extraperitoneal approach (EPA) for the resection of primary retroperitoneal tumors (PRT). MethodsForty six cases undergoing resection of PRT were analyzed retrospectively, of which, 26 cases were through transabdominal approach (TAA group) and 20 through EPA. ResultsThe postoperative complications in EPA group was lower than in TAA group (2/20 vs. 11/26, P

OBJECTIVETo study the chemical constituents from the seeds of A. katsumadai, and their inhibition on NF-kappaB activation and antitumor effect.

METHODTweleve compounds were isolated from the seeds of Aplinia katsumadai on repeated column chromatography on silica gel, and Sephadex LH-20, and their structures were determined mainly by means of MS and NMR techniques; and their inhibition on NF-kappaB activation and antitumor effect were also tested by High-Content Screening (image-based) with immunofluorescent probe and MTT method, respectively.

RESULTFrom the EtOAC fraction of the seeds of A. katsumadai 12 compounds were isolated and identified as follows: (3S,5S)-trans-3,5-dihydroxy-1 ,7-diphenyl-hept-1-ene (1), (3R,5S)-trans-3,5-dihydroxy-1,7-diphenyl-hept-1-ene (2), 5-hydroxy-1,7-diphenyl-hepta-6-en-3-one (3), cardamonin (4), alpinetin(5), pinocembrin(6), pinostrobin(7), naringenin (8), (+)-catechin(9), chrysin(10), rutin(11) and 2,4- dihydroxy-6-phenethl-benzinic acid methyl ester (12). Compound 14 showed inhibitory effect on NF-kappaB activation with the IC50 values as 14.8, 16.5, 23.2 and 7. 5 micromol x L(-1), respectively. Compound 4 displayed cytotoxicity against leukemia K562 cells and human hepatoma cell line SMMC-7721 with IC50 values as 3.2 and 3.5 mg x L(-1), and compound 6 showed moderate cytotoxicity against SMMC-7721 with the IC50 value as 18.3 mg x L(-1).

CONCLUSIONCompounds 7-12 were isolated from A. katsumadai for the first time and Compound 12 were isolated from the genus Aplinia for the first time; compound 4 has the activity of anti-tumor and NF-kappaB activation inhibition, compounds 1-3 have the activity NF-KB activation inhibition.

Alpinia ; chemistry ; Antineoplastic Agents, Phytogenic ; analysis ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Down-Regulation ; Humans ; NF-kappa B ; antagonists & inhibitors ; metabolism ; Plant Extracts ; analysis ; isolation & purification ; pharmacology ; Seeds ; chemistry

OBJECTIVE：To compare the chemical compo sition difference in methanol and petroleum ether fraction from Curcuma longa of different habitats. METHODS ：The ultrasonic method was used to extract C. longa from 7 defferent producingareas（S1-S7），and methanol and petroleum ether fraction were obtained and calculated yield. The curcumin compounds in methanol fraction were determined by LC-MS ；The chemical components in petroleum ether fraction were analyzed by GC-MS ， and the relative percentage content was determined by peak area normalization method after determining its structure by comparing NIST 2005 standard mass spectra and Wiley 275 standard mass spectra. SPSS 25.0 software was used for principle component analysis（PCA）and cluster analysis of relative percentage content of common components in petroleum ether fraction from C. longa of different habitats. At the same time ，the influence of latitude of the habitats on the content of total tumerone （by tumerone and ar-tumerone ）was analyzed. RESULTS ：The yield of methanol fraction were 1.35％-8.90％ from C. longa of 7 habitats；the yield of petroleum ether fraction were 0.81％-4.90％，which were the highest in C. longa from Longyan of Fujian Province. There was no significant difference in the relative content of curcumin compounds（reference peak area ）from S 1，S3-S7，which was in descending order as follows as curcumin ＞desmethoxycurcumin＞bisdemethoxycurcumin. There was slightly different in curcumin compounds of C. longa from S 2，mainly manifesting as the content of bisdemethoxycurcumin was higher than that from other producing areas. Totally 48 chemical compositions were identified from petroleum ether fraction in C. longa from different habitats ， mainly being sesquiterpenoids and monoterpenoids. 23，10，15，18，11，14，15 chemical compositions were identified from S1-S7，accounting for 94.49％，96.09％，95.66％，98.98％，99.24％，89.05％ and 97.27％. There were 4 common compositions in C. longa from different habitats ，which were tumerone （17.90％-43.07％），ar-tumerone（6.97％-33.66％），（6R，7R）-bisabolone （1.60％-4.28％），curlone（6.80％-20.63％）. PCA analysis showed that accumulative contribution rate of former 6 principle components was 100％. Cluster analysis showed that S1，S2， S6 was clustered into a category ，respecrively；and others intoa category. Total content of total tumerone decreased first and then increased as the increase of latitude ，which was the highest in Mianyang of Sichuan province （64.28％）and the lowest in Zhangzhou of Fujian province （26.92％）. CONCLUSIONS ： There are difference in composition and content of methanol and petroleum ether fractions in C. longa from different habitats.