Objective To retrospectively review the clinical data of acute oral paraquat (PQ) poisoning patients and the relationship of early serum creatinine leveles with respiratory failure , to predict and guide the clinical diagnosis and treatment .Methods Seventy-two PQ poisoning patients from the 2008 to 2012 , according to the occurrence of respiratory failure were divided into two groups , group A:patients with respiratory failure (28 cases);and group B:patients without respiratory failure(44 cases).The first serum creatinine detection early after poisoning as the basic value and the highest value within 48 hours, and the difference between the two ratios was compared .Then the sensitivity, specificity, accuracy, Youden index, receiver operating characteristic(ROC) area under the curve(AUC) were also calculated, and the ROC AUC was drawn.Results The creatinine basic value ,the highest valve and their ratio were obtained , and the difference was statistically significant .The best predictor boarder of creatinine basic value was 53 μmol/L, with forecasting moderate effect,and that of the highest value ,their ratio and the creatinine difference value was 183μmol/L, 3.39 and 113μmol/L, respectively.Conclusion The higher the early serum creatinine level rises , the higher the risk of respiratory failure increa-ses after PQ poisoning .The basic value , the highest value within 48 hours and their difference and ratio could predict the possibility of respiratory failure after PQ intoxication .

Objective To find the correlative factors which induce breath failure in acute tetramine poisoning cases, and to work out a right treatment scheme for these cases. Methods 47 cases were clinically diagnosed as slight, middling and severe poisoning, separately. The tetramine concentration in blood was compared between the three groups to reveal the relationship between blood concentration of tetramine and breath failure. Patients with breath failure were treated with muscle relaxant plus mechanical ventilation. Repeated hemoperfusions were given to the severe poisoning patients. Results No breath failure occured in both slight and middling groups, while in severe poisoning group, breath failure happened in 33.3% (4/12) patients aged below 15, and in 31.8% (7/22) adult patients aged above 15. Repeated hemoperfusion declined the tetramine concentration in blood, and gave a better effect than with CVVH or CVVHD treatment. muscle relaxant plus mechanical ventilation may control the symptom of twitching completely, and prevent from breath failure effectively. Conclusion Breath failure in acute tetramine poisoning cases was induced by systemic twitching and high blood tetramine concentration. It is very important to grade the poisoning cases according to poisoning degree. Repeated hemoperfusion is the best for blood purification. muscle relaxant plus mechanical ventilation may reduce the mortality effectively.

AIM To evaluate the effects of diazoxide on the structures and functions of the mitochondria after hypoxia injury. METHODS A myocardial hypoxia injury model on rat is established by subcutaneous injection of isoproterenol (ISO) 5 mg?kg -1. The degree of oxidative phosphorylation, membrane lipid fluidity (LFU), phospholipidase A 2 activity, and phosphlipid content of mitochondria were observed after hypoxia injury. RESULTS Compared with the controls, the levels of RCR, LFU and phosphlipid content were decreased 20.8% (P

Objective:To investigate the protective effect of racanisodamine on lung injury in mice exposed to irradiation.Methods:C57BL/6 mice were randomly divided into control group, racanisodamine group, 18 Gy irradiation group (model group) and racanisodamine combined with 18 Gy irradiation group (treatment group), with 5 mice in each group. The mice in the treatment group received racanisodamine (5 mg/kg) intraperitoneally 3 d before irradiation and contained the whole experiments. Then, single chest irradiation of 18 Gy X-rays was performed both in the model and treatment groups. The racanisodamine group and treatment group received racanisodamine intraperitoneally once a day until 6 weeks after irradiation. The mice were killed at 6 weeks after irradiation. The lung histopathology was observed by HE staining. Serum and bronchial alveolar lavage fluid (BALF) inflammatory cytokines such as TNF-α, IL-1β and IL-6 were determined by ELISA method. Cell senescence was detected by SA-β-Gal staining. The expressions of Nrf2, p-Nrf2 and p62 in lung tissue were performed by immunehistochemistry and Western blot assays.Results:Compared with the model group, the scores of HE staining were decreased ( t=8.66, P<0.01), the number of infiltrated inflammatory cells in BALF were decreased ( t=10.70, P<0.01), and protein concentration in BALF had lower levels ( t=6.75, P<0.01), the serum TNF-α, IL-1β and IL-6 were decreased significantly ( t=8.17, 4.58, 6.54, P<0.01), the activity of SA-β-gal was decreased, and the expressions of Nrf2, p-Nrf2 were enhanced ( t=6.42, 7.30, P<0.01), while the expression of p62 was reduced ( t=4.62, P<0.01) in the treatment group. Conclusions:Racanisodamine plays the protective effect of radiation-induced lung injury by alleviating inflammation associating with the activating of Nrf2-related pathway, which reversed radiation-induced cell senescence.