Objective To explore the application of thinprep cytology test thinprep cytologic test ( TCT) and the serum squa-mous cell carcinoma antigen ( SCC-Ag) measurement in the cervical lesions screening .Methods TCT and serum SCC-Ag measure-ment were performed in 58 patients with cervical lesions .Based on pathology diagnosis , specificity and sensitivity of these two screen-ing methods were calculated .Results Sensitivity and specificity of TCT detection were 75%and 65.8%, the sensitivity and specific-ity of SCC-Ag detection was 60%and 86.9%, respectively, and specificity of combined detection were 55%and 89.4%, respective-ly.There was statistical significance in TCT test and SCC-Ag test ( P <0.05 ) , the difference had statistical significance of combined detection of both with a single TCT to detect differences ( P <0.05 ) .Conclusions The combination of TCT and SCC-Ag detection can improve specificity and there was a certain clinical application value in the screening of cervical lesions .

Objective To observe the effect of several antihistamines,including epinastine,on the release of leukotriene B4(LTB4),interleukin 4(IL-4)and interleukin 5(IL-5)from spleen lymphocytes of allergic mice challenged by ovalbumin(OVA).Methods OVA-sensitized BALB/c mice were used as animal model of allergy.Spleen lymphocytes isolated from these mice were treated with epinastine(0.1,1.0,10 μmol/L),ebastine(10 μmol/L),cetirizine(10 μmol/L),and dexamethasone(1.0 μmol/L),respectively,followed by incubation with OVA.Those cells without pretreatment with antihistamines served as the control.One hour after the challenge,enzyme-linked immunosorbent assay(ELISA)was applied to measure the level of LTB4,IL-4 and IL-5 in the cell culture supernates.Results Following the incubation with OVA,the levels of LTB4,IL-4 and IL-5 increased significantly in culture supernates of control spleen lymphocytes.Epinastine significantly inhibited the release of LTB4,IL-4 and IL-5 in a dose-dependent manner.At the concentration of 10 μmol/L,the inhibitory effect of epinastine was more potent than that of ebastine and cetirizine for IL-5 and LTB4 release(all P＜0.05):for IL-4,the efrect of epinastine was similar to that of ebastine (P＞0.05)but more potent than that of cetirizine(P＜0.05).Conclusion Epinastine has a potent anti-inflammatory effect.

Antipsychotic Agents ; therapeutic use ; Brain ; physiopathology ; Cognition Disorders ; genetics ; Gene-Environment Interaction ; Genetic Variation ; genetics ; Humans ; Schizophrenia ; genetics ; physiopathology ; Schizophrenic Psychology ; Social Behavior ; Thinking ; physiology

Acute Disease ; Adult ; Aged ; Dengue ; diagnosis ; Diagnosis, Differential ; HIV Infections ; diagnosis ; HIV-1 ; immunology ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Singapore ; Young Adult

Dust ; prevention & control ; Germany ; Humans ; Metallurgy ; Occupational Exposure ; prevention & control ; Occupational Health ; Population Surveillance ; Silicon ; adverse effects

Objective To evaluate the therapeutic effeet of transurethral non-contact laser in the bladder neck stricture. Methods Q-9000 laser generator was used.The Nd-YAG laser was located to the bladder neck by laser fiber(USA).The bladder neck was cut on the point of 12 and 6 in all 39 cases. Results The maximum urinary flow was increased from (8.2?2.4)ml/s to (16.5?3.2)ml/s after operation. Conclusions Transurethral non-contact laser is effective for the treatment of bladder neck stricture.

The morbidity and nortality of esophageal carcinoma showed an upward trend in recent years.Neoadjuvant radiochemotherapy is the nain mode of treatment for advanced esophageal carcinoma,including neoadjuvant chemotherapy,neoadjuvant radiotherapy and neoadjuvant radiochemotherapy.However,there are still many controversies on efficacies and advantages or disadvantages of treatment mode.In this article,authors will investigate the present situations and future direction of neoadjuvant therapy for locally advanced esophageal carcinoma.

Objective To investigate the association between serum non-organ-specific autoantibodies and polycystic ovary syn-drome(PCOS).Methods A total of 69 patients with PCOS in child-bearing period treated in our hospital were selected as the study group and contemporaneous 69 healthy child-bearing year women were selected as the control group.Serum level of antinuclear anti-bodies (ANA)was measured by ELISA,serum level of anti-dsDNA was measured by colloidal gold spot infiltration assay and the extractable nuclear antigen (ENA)auto-antibodies profiles were measured by Western blot.Results Serum levels of ANA and an-ti-dsDNA antibodies in the study group were significantly elevated compared with the control group with statistical difference.Con-clusion Serum autoantibody positive exists in the patients with PCOS.

Objective To understand the city residents' iodine nutrition level after implementation of a new iodine concentration standard.Methods Using random sampling,four counties were selected to monitor iodine nutrition level;in every county 50 children aged 8-l0 and 20 pregnant women were selected as monitoring subjects;B ultrasound scan was used to detect goiter and children urinary iodine and salt iodine levels were tested;urinary iodine concentration of pregnant women and nursing mothers was determined.Results Thyroid of 200 children aged 8-10 years were detected,the enlargement rate of thyroid gland was 1％ (2/200);200 salt samples were tested,qualified iodized salt consumption rate was 96％ (192/200);200 copies of children urine samples were tested,the median of urinary iodine was 229.3 μg/L;80 copies urine samples of pregnant women were tested,urinary iodine median was 183.7 μg/L.Conclusions After implementation of a new standard of salt iodine content,the iodine nutrition level of children in the four counties (districts) of Zunyi is still high,but the level of iodine nutrition of pregnant women appears imbalanced.We suggest to supply different concentrations of iodized salt for different groups of people,to strengthen the monitoring,and to prevent iodine deficiency disorders.

Objective To investigate the transfection and expression of pcDNA3 1-VEGF 165 plasmid in mammalian cells. Methods pcDNA3 1-VEGF 165 plasmid was transfected into CHO cells by electroporation and the positive clones were screened by G418. The expression of VEGF 165 gene in the transfected CHO cells were detected by RT-PCR, ELISA and western blotting analysis. The biologic activities of VEGF 165 secreted by the transfected CHO cells were measured by Mile's assay and MTT method. Results G418-resistant CHO cell clones were obtained. RT-PCR, ELISA and western blotting analysis showed that there were the transcription and translation of VEGF 165 gene in anti-G418 CHO cells. The mile's assay showed that the expressed VEGF 165 could increase the vessel permeability, and MTT assay proved that it could promote the proliferation of endothelial cells. Conclusion pcDNA3 1-VEGF 165 plasmid could express VEGF 165 with biological activities in mammalian cells.