Surgery in combination with chemotherapy and radiotherapy is the standard of lung cancer treatment,but postoperative recurrence is very common which usually leads to higher mortality and lower life quality. Immunotherapy on post-operative patients fully mobilizes the body's defense mechanisms,activates the immune cells,and kills residual cancer cells. Current research on lung cancer immunotherapy mainly includes four categories:adoptive immunotherapy,dendritic cell vaccine,non-specific antigen immune therapy and anti-gen-specific vaccine. These studies show lung cancer immunotherapy intervention can effectively reduce postop-erative residual cancer cells,reduce postoperative recurrence rate,prolong survival,significantly improve the prognosis,and is worth spreading in clinical practice.

Bariatric surgery began in 1950s.The rapid increase of obese patients and development of laparoseopic techniques lead to popularity of bariatric surgery all over the world in 1990s.Current mainstream of bariatric surgeries include laparoscopic adjustable gastric banding,sleeve gastrectomy,Roux-en-Y gastric bypass and biliopancreatic diversion.In this review,the efficacy of different surgical procedures was compared in the aspects of metabolic disorder remission and incidence of operation complications and mortality.Efficacy of bariatric surgery on the alleviation of metabolic disorders (including hypergycemia,hyperlipidemia,hypercholesterolemia),individual treatment and interdisciplinary cooperation should be emphasized.

Insulin-like growth factor binding proteins (IGFBPs) are crucial to cell growth, development, proliferation, and apoptosis in humans. Among IGFBPs, IGFBP2 is recognized as a regulator of insulin-like growth factor (IGF). Besides binding with IGF, IGFBP2 also interacts with extracellular matrix through its specific structure. IGFBP2 promotes the malignant phenotype of tumor by activating several important intracellular signal pathways. IGFBP2 is specifically overexpressed in several malignant tumors, and this overexpression is correlated with patient prognosis. IGFBP2 is regarded as a potential biomarker and a therapeutic target. This review briefly summarizes the latest progress of research on the role of IGFBP2 in tumor malignant biological behavior and its clinical application.

Objective To discuss the therapentic efficacy of hyperbaric oxygen combined with PC program in newly diagnosed epithelial ovarian cancer patients. Methods Fifty-eight patients with epithelial ovarian cancer were divided into two groups by random digits table: HBO group(30 cases) and PC group(28cases). HBO group were exposed to hyperbaric oxygen of 2 standard atmospheric pressure 60 min, then given chemotherapy 25-30 min after extravehicular: cyclophosphamide 1000 mg/m2 + cisplatin 75 mg/m2.PC group with the same regimen without hyperbaric oxygen therapy were analyzed. The two groups were compared in the efficacy and 3-year survival rate, progression-free survival and adverse reactions. Results The total effective rate, not controlled rate, recurrence rate,recurrence time, 3-year survival rate in HBO group [83.3%(25/30),6.7%(2/30),33.3%(10/30), (21.0 ± 0.8) months,43.3%(13/30)] were better than those in PC group [67.9% (19/28), 17.9% (5/28), 46.4% (13/28), (18.0 ± 0.6) months, 17.9% (5/28)] (P ＜0.05), progression-free survival and overall survival time in HBO group were longer than those in PC group (P ＜0.05) and adverse reactions rate in HBO group was lower than that in PC group (P ＜0.05).Conclusions The hyperbaric oxygen combined with PC programs are better than PC programs in advanced epithelial ovarian cancer chemotherapy response rate, progression-free survival time and 3-year survival rates in ovarian cancer adjuvant chemotherapy. HBO can significantly reduce the PC's hematological toxicity and toxicity of the digestive system.

Objective To explore the risk factors of malignant glaucoma of primary angle-closure glaucoma post operation.Methods The clinical data of 500 patients (705 eyes) with primary angle-closure glaucoma who received glaucoma surgery were retrospectivly analyzed.25 patients (30 eyes) with malignant glaucoma after surgery were selected as observation group,475 patients (675 eyes) with no malignant glaucoma after surgery were selected as the control group.The gender,age,type of glaucoma,preoperative intraocular pressure,anterior chamber depth,relative position of the lens , lens thickness , length of optic axis , corner structure and operation method of the two groups were recorded.Single factor analysis was conducted by chi-square test and t test,multiple factors analysis was conducted by Logistic regression analysis.Results Single factor analysis showed that there were significant differences in gender,age,glaucoma types,intraocular pressure and ocular axis length in the two groups (χ2 =5.394,4.785,6.475, 4.907,4.890,all P<0.05).Multivariable Logistic regression analysis results showed that age (OR=3.774,95%CI 1.765-3.774,P=0.004),and length of optic axis (OR=2.684,95% CI 1.363 to 6.953,P=0.005) were independent risk factors of primary angle -closure glaucoma post operation.Conclusion Women, younger age, chronic angle -closure glaucoma, continuous high intraocular pressure, corner completely closed, shallow anterior chamber,crystals thinner and shorter optic axis of primary angle -closure glaucoma patients prone to malignant glaucoma postoperation,especially younger age and optic axis short patients,should be given enough attention.

Objective To investigate the etiology, clinical features, diagnosis and treatment of regional portal hypertension (RPH).Methods The clinical data of 26 patients with RPH treated in Beijing Chaoyang Hospital Affiliated to Capital Medical University between January 2005 and June 2010 were analyzed with retrospective analysis.The first symptom, routine analysis of blood, liver function test, hepatitis B and C markers, tumor markers, abdominal ultrasound, abdominal enhanced CT, endoscopy findings of 26 patients and the results of abdominal CT angiography (CTA) of 16cases were analyzed.Results Pancreatic disease (18 cases) was the leading cause of RPH.The main clinical manifestations of splenomegaly in 26 cases, irregularly abdominal pain in 14 cases, and upper gastrointestinal bleeding in 10 cases.Isolated gastric varices were revealed by endoscopy in 25 cases,complicated with lower esophageal varices in 1 case.4 cases with endoscopic tissue glue injection in gastric variceal bleeding, splenectomy in 4 cases, 2 cases with splenectomy and pericardialdevascularization, 2 cases with splenectomy, pancreatic tail resection and spleno-renal shunt, 3 cases with splenic embolization treatment.Conclusions RPH often accompanied by pancreatic disease,manifested as splenomegaly, hypersplenism, but normal liver function, absence of liver cirrhosis.Isolated gastric varices is the characteristic features of RPH.RPH caused by benign diseases is curable.Splenectomy is more effective than simple endoscopic hemostasis in RPH associated with gastrointestinal bleeding.

Objective To explore the influence of anti-syphilis treatment to pregnant women with lower titer seroresistance on infantile serum,so as to provide the guidance for clinical diagnosis and treatment.Methods Totally 134 cases of pregnant women with lower titer syphilis serofast reaction were divided into treatment group (75 cases) and untreated group(59 cases) according to whether received anti-syphilis treatment during their pregnancy.The change of syphilis serology toluidine red unheated serum test (TRUST) and Treponema pallidum particle assay (TPPA) for the two groups mothers and babies were compared.Results (1) The first detection of TRUST titers between the two groups of pregnant women did not show statistically significant difference (x2 =0.520,P ＞ 0.05).(2) Among the 134 neonates,20 cases(14.9％) were negetive for both TRUST and TPPA,23 cases (17.2％) were Treponema pallidum particle assay(TPPA) positive only,91 cases (67.9％) were positive for both TRUST and TPPA and showed lower or equivalent TRUST titers compared to their mothers,without significant differences between the two groups(x2 =0.892,P ＞ 0.05).(3)In the two groups of babies with the same TRUST titers,the seroreversion time of TRUST showed no significant differences(P =0.229,0.309,1.000).The negative time of TRUST in infants with neonatal higher titer was later than those with neonatal lower titer in the two groups (all P ＜ 0.05).The infant with TRUST + showed longer duration than those with neonatal TRUST-in TPPA seroreversion(all P ＜ 0.05).The seroreversion time of TPPA in infants with neonatal TRUST titer of 1:4 in untreated group was later than that in treatment group[(14.1 ±1.4)months vs.(12.5 ±1.1)months,t =2.900,P =0.010].Conclusion The treatment for mothers with lower titer seroresistance in pregnant period had no influence in the positive rate of TRUST and TPPA in the neonates and seroreversion time of TRUST in infant.It may have certain effect to shorten the seroreversion time of TPPA in infant with high TRUST titer by the treatment.

Leukemia is a kind of cloning disease from malignant hematopoietic stem cells. Its patho-genesis may have relationship with proliferation out of control,dysdiffreentiation and inhibition of apoptosis about leukemia cells. The study confirms that CD44 is expressed in leukemia cells,promoting the occurrence and development of leukemia through participation in cell adhension,proliferation,migration and invasion behavior. In vitro experiment shows that CD44 targeted therapies can effectively kill leukemia cells,and reduce the toxicity to normal tissue cells. Therefore,aiming at the design of the anticancer drugs targeted for CD44 is expected to be used in clinical treatment of leukemia.

Objective To investigate new methods of osteonecrosis of femoral head (ONFH) with retrospective analysis of the clinical cases with technique of rotary cutting pressure-relief,impacted graft,and tantalum rod supporting treatment of early-stage femoral head necrosis.Methods From June 2010 to June 2014,32 cases (38 hips,23 males and 9 females) with ONFH were treated with technique of rotary cutting pressure-relief,impacted graft,and tantalum rod supporting,with the mean age of 24 ～ 52 (36.2 ± 3.8) years old.According to the Association Research Circulation Osseous (ARCO) classification,there were 11 hips on Ⅱ A stage,12 hips Ⅱ B,8 hips Ⅱ c,7 hips Ⅲ A.The averaged Harris score of all cases was 65 ～ 81 (72.5 ± 5.8) points.All cases were cured with minimally invasive technique,rotary cutter pressure-relief in femoral head,thoroughly remove sequestrum and implant allogeneic bone.Postoperative patients laid in bed for 1 month,after 1 month hold crutch gradually began to functional training.Postoperative 1 month,X ray film was reviewed every 3 months.According to the clinical symptoms,imaging findings,Harris scoring system was graded to evaluate the effectiveness.Results All the cases were followed up for average of 12 ～32 (20.2 ± 2.6) months,31 hips Kept hips succeed,hips pain; lame line symptoms were reduced or disappeared; x-ray showed femoral head necrosis area formed new bone,Harris scoring was upgraded to 75 ～ 95 (87.7 ± 6.4) points ; and seven hips failed with operation of total hip arthroplasty.The overall good rate was promoted from 26.3％ to 84.2％.Conclusions The technique treating for early-stagefemoral head necrosis with minimally invasive,rotary cutting pressure-relief,impacted graft,and tantalum rod supporting had a good short-term effects.

Objective To investigate the functions of triple point-mutants of hypoxia-inducible factor 1α(HIF1α)in angiogenesis in bone defect region under normoxic conditions.Methods Triple point-mutations (the 402,564 and 803 amino acids)in HIF1α coding sequence (CDS)were induced.The triple mutant of HIF1α(402/564/803)was inserted into the adenovirus pAdEasy-1 system to complete viral packaging and titer measurements. The wild-type HIF1α gene and the empty adenovirus vector were packaged in the same way.For the in vitro experiment,the rabbit bone marrow mesenchymal stem cells (MSCs)were divided into four experimental groups:A,MSCs infected with viral solution containing mutant HIF1α;B,MSCs infected with viral solution containing wild-type HIF1α;C,MSCs infected with viral solution without any HIF1α;and D,MSCs without viral infection. The efficiency of infection was observed by the expression of human renilla reniformis green fluorescent protein (hrGFP).The expression levels of HIF1α mRNA and protein in infected cells in each experimental group were measured.For the in vivo experiment,the MSCs were divided into the same four groups and infected with the virus solutions from each group and cultured under normoxic conditions.At 72h after the infection,the MSCs were used as seed cells and transplanted into Apatite-wollastonite magnetic bioactive glass-ceramic (AW MGC)vector to construct artificial tissue-engineering scaffolds,and then the scaffolds were implanted into the in vivo rabbit radial bone defect model.The animals from each group were sacrificed 8 weeks after the surgery and the tissues from the implantation region were harvested for evaluation of angiogenesis.Results The 402,564 and 803 amino acids in CDS area were point mutated into alanine;three types of recombinant adenovirus were successfully constructed, packaged and characterized.The expression levels of HIF1αmRNA were significantly higher in Group A and Group B than in Group C and Group D (P <0.05 ).There was no significant difference between Group A and Group B group or between Group C and Group D (P >0.05 ).The HIF1α protein expression in Group A was significantly higher than that in the other three groups (P <0.05);however,there was no significant difference among groups B,C and D (P >0.05).There was prominent angiogenesis in bone defect region in Group A,but not in the other three groups.Conclusion ① Triple point-mutants of HIF1αefficiently expressed functional proteins under normoxic conditions.② Triple point-mutants of HIF1αeffectively promoted in vivo angiogenesis in bone defect region.