1.The effect of hyperuricemia on nuclear factor-κB in myocardial tissue Mn superoxide dismutases activity in mitochondria of rats
Chunyan HAO ; Hubin DUAN ; Junfeng ZHANG
Chinese Journal of Rheumatology 2017;21(3):162-166,封3
Objective To investigate the effect of hyperuricemia (HUA) on nuclear factor-κB (NF-κB) in myocardial tissue,Mn superoxide dismutases (Mn-SOD) activity in mitochondria,cardiac function of rats with HUA.Methods Sixty SD male rats,including 30 young rats and 30 aged rats,were randomly divided into four groups:the young control group,the young HUA group,the aged control group and the aged HUA group.Fifteen rats were in each group.The HUA rat models were set up by lavage method with yeast extract and ethambutol.Left ventricular systolic pressure (LVSP),left ventricular end-diastolic pressure (LVEDP) and left ventricular pressure at the end of the rising and falling maximum velocity (+dp/dtmax) were determined by intubation in the left ventricle with cardiac catheter.Mn-SOD activity in the mitochondria of rat myocardial tissue was detected by chemical colorimetry.The expressions of NF-κB in rats myocardial tissue were measured by immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR).Differences between groups were analyzed with One way analysis of variance,Student-Newman-Keuls q test was used to compare the mean of multiple samples.Results Hyperuricemia had no significant effect on the cardiac function of HUA groups (P>0.05).But the gene and protein expression of NF-κB in myocardial tissue and Mn-SOD activity of mitochondria were impacted by HUA.Both the gene and protein expression of NF-κB and the activity of Mn-SOD in HUA groups were increased (F=85.428 4,120.683 0 and 398.228 3,P<0.01).Furthermore,the increase of gene and protein expression of NF-κB and the Mn-SOD activity in rats myocardial tissue of young HUA group were higher than those in aged HUA group (q=6.818 6,10.693 6 and 18.877 9,P<0.05).Conclusion Hyperuricemia may cause inflammation and increase oxygen free radicals in myocardial tissue on one hand,and it may reduce inflammation and protect the myocardial tissue by increasing the activity of Mn-SOD in myocardial mitochondria of HUA rats,and removing the oxygen free radicals on the other hand.The response to uric acid in young HUA group is stronger than that in the aged HUA group.
2.Expression level of nuclear factor-kappa,substance P and aquaporin 4 gene in the joint synovium and the change of discharge frequency of pain sensitive neuron in the ventral posteriolateral thalamic nuclens of acute gout rats
Chunyan HAO ; Hubin DUAN ; Junfeng ZHANG
Chinese Journal of Rheumatology 2010;14(2):102-106
Objective To investigate the expression level of nuclear factor-kappa(NF-κB),substance P(SP)and aquaporin 4(AQP4)gene and the change of discharge frequency of pain sensitive neurons (PSN)in the ventral posteriolateral thalamic nucleus(VPL)of acute gout(AG)rats.The neuro-genic inflammation of AG was explored.Methods Forty-eight rats were randomly divided into two groups:the control group and the AG group. According to the time interval after injection of monosodiumurate(MSU)into the the unilateral ankle joint,the AG group was subdivided into seven groups,ie.0.5 hours group,2 hours group,6 hours group,12 hours group,24 hours group,48 hours group and 72 hours group.There were 6 rats in each group.After recording of the discharge frequency of PSN in rats VPL nucleus,the expression level of NF-κB,SP and AQP4 gene in the rats joint synovium were evaluated at mRNA level by PCR in above mentioned time.Results In the 0.5 hours group,the discharge frequency of PSN in the rats VPL nueleus and the expression level of SP gene in the rats joint synovium had increased immediately after the injection of MSU(P<0.05).In the 6 hours group,they reached the peak level(P<0.05),and in the 12 hours group,they began to decrease gradually(P<0.05).In the 0.5 hours group,the expression level of NF-κB and AQP4 gene increased after the injection of MSU(P<0.05).However,their peak level presented at the 12 hours(P<0.05),and they decreaged after 24 hours(P<0.05).The statistical analysis of correlation had shown that there were positive correlation among the expression level of NF-κB,substance P.AQP4 gene and the change of discharge frequency of PSN in the rats VPL nucleus.Conclusion The discharge frequency of PSN in the rats VPL and the expression level of SP gene in the rats ioint synovium can be used to evaluate the Severity of pain in the AG rats.The expression level of NF-κB and SP gene can reflect the severity of neurogenic intlammation.We can know the severity of edema of the joint synovium by detecting the expression level of AQP4 gene.Pain and neurogenic inflammation play an important role in the pathogenesis of AG.Combingelectrophysiology and biochemical technique can shade light on the pathogenesis of AG from different aspects.In the meantime,it may provide a new method for developing new drugs and new approaches for clinical treatment.
3.Mulit personal computer storage system: the solution of PACS storage
Fude HAO ; Xinlin DUAN ; Qiyong GUO
Chinese Journal of Radiology 1994;0(06):-
Objective According to the characteristics of digital medicine and demand of digitized management in hospital, we establish a storage system which is cheap, highly expansible, and reliable. Methods The multi personal computer storage system (MPCSS) was constructed by assembling the hardwares and softwares. The image data were archived from major server to storage PC by using NEUSOFT PACS archiving manage system and backuped on storage PC. We simulated the situation that the data on storage PC was lost and restored the data. We also expanded the storage system to enlarge its capacity. Results Average transfer rate from MPCSS was 27 7 Mbit/s (1 byte=8 bit); average cost for this system was 74 RMB/G; six cases in the 187 repeated reading of 100 patients failed. MPCSS can store backup and restore the image data, and can expand the storage size. Conclusion MPCSS is very cheap compared with other high capacity systems or devices. It is feasible and suitable for digital image storage.
4.Effects of Spasfon on course of labor
Yan HAO ; Guirong ZHAI ; Aihong DUAN
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
0.05). Conclusion Spasfon can effectively improve cervical dilatation during labor and it is well tolerated by both mother and newborn.
5.Construction of cDNA library from adults of Anopheles dirus
Hongxing HAO ; Wenyue XU ; Jianhua DUAN ; Mingshu KUANG ; Fusheng HUANG ;
Journal of Third Military Medical University 2003;0(11):-
Objective To construct cDNA library from adults of Anopheles dirus for cloning the immune genes or related genes for malaria parasites development. Methods The mRNA of adult Anopheles dirus was isolated. The library was constructed by using the Zap Express vector(Stratagene) and the quality was evaluated. Results The efficiency of the library was 2.1?10 6 pfu/ml with 98% clones positive. The average length of the insert fragment was over 1 kb. Conclusion cDNA library of adult Anopheles dirus with high efficiency can be constructed by using the Zap Express library construction Kit (Stratagene).
6.The value of MR perfusion weighted imaging in normal and abnormal kidneys
Hao SHI ; Ruiping DUAN ; Yongping SUN ; Yiyong XING ; Hongyu DING
Chinese Journal of Radiology 2008;42(10):1064-1068
Objective To explore the characteristics and the clinical application of MR perfusion weighted imaging(PWI)in the normal kidneys and the renal diseases.Methods Thirty-one subjects including 9 cases without urinary diseases,14 cases with renal carcinoma,6 cases with renal cyst and 2 cases witll renal tuberculosis who had been examined with T1WI,T2WI and PWI were analyzed retrospectively.All the data were processed by a workstation to obtain time-signal intensity curves,color perfusion maps and relative perfusion value.The relative renal blood volume(RBV),relative renal blood flow(RBF),mean transition time(MTT)and the time to peak(TTP)in the normal renal cortex and medulla and the renal lesions were calculated.Comparisons between the right and the left normal kidneys,and between the cortex and the medulla of the normal kidneys were performed using t test,and comparisons between the normal and the abnormal kidneys were performed using q test.Results Relative RBV and relative RBF of the cortex were 1.33±0.08 and 1.44±0.09 respectively,and for medulla were 0.58±0.05 and 0.78±0.13 respectively(t=9.2241 and 5.0336,P<0.01);MTT(1.11±0.08)and TTP (1.04±0.06)of the cortex has no difference with that of the medulla(0.97±0.04 and 0.94±0.03)in the normal kidneys(t=2.2551 and 2.2613.P>0.05).The values of relative RBF of the renal carcinoma (1.35±0.34)were significantly higher than that of the normal tissues(1.02±0.06)(q=3.0882,P<0.01).Conclusion PWI is able to demonstrate the hemodynamic change of the normal renal tissues and the renal lesions,and it maybe an ideal method for showing the functional changes of the kidney and for differentiating the renal diseases.
7.The analysis of correlation between the performance of HBV markers and AFU,ALT,GGT
Chaowei OU ; Yunlin DUAN ; Yaojuan LIU ; Yanqiang HAO
Chinese Journal of Primary Medicine and Pharmacy 2009;16(11):1987-1988
Objective To investigate the correlation between hepatitis B virus(HBV)markers and AFU,ALT,GGT.Methods 570 eases of HBV-infected people were divided into 4 groups according to the different performance modes.150 healthy people were considered as control group.rate method was used to detect AFU,ALT and GGT.Estimate the differences between the groups were compared.Results The comparison between the AFU,ALT,GGT in different performance mode of HBV and the control group showed:the differences of group 1,group 2 and group 3 were statistically significant(P<0.01),the difference of group 4 was not significant(P>0.01).The correlation between AFU,ALT,GGT of different performance mode showed:the changes of AFU and ALT,GGT in group 1,group 2 and group 3 were positively change-related.But the changes of AFU,ALT and GGT were not clearly related.The distribution of the rise of serum enzyme activity in different performance mode showed:the AFU,ALT and GGT rised in different proportion in group 1,group 2 and group 3.Conclusions The AFU,ALT and GGT activity of HBVinfected persons should be detected at the same time,and evaluate the damage of liver cells.This will do help to predict the progression of hepatitis.
8.High glucose stimulates the expression of transforming growth factor-β1 and fibronectin via SREBP-1 in HKC cells
Jun HAO ; Jinping ZHANG ; Jiangbo GU ; Shuxia LIU ; Huijun DUAN
Chinese Journal of Endocrinology and Metabolism 2009;25(4):442-444
significantly increased (all P < 0.01). Following SREBP-1 was down-regulated by siRNA, high glucose-stimulated TGF-β1 and FN protein expressions were decreased by 17.9% and 24.6% ,respectively(all P<0.01).
9.Research of real-time shear wave elastography in liver stiffness of normal children
Qingrong XIA ; Xingxing DUAN ; Hao LI ; Jingbo HE
Chinese Journal of Medical Imaging Technology 2017;33(9):1353-1356
Objective To explore the clinical value of real-time shear wave elastography (SWE) in liver tissue stiffness of normal children.Methods Totally 243 normal children were examined by SWE.The data of liver stiffness measurement (LSM) were recorded and analyzed.The differences of LSM between different genders and hepatic segments were compared.According to the age,243 normal children were divided into pre-school group,school age group and adolescent group;and the differences of LSM between 3 groups were compared.The consistency was analyzed in different operators.Results The mean value of LSM in normal children was (4.21±0.70)kPa,and the 95% confidence interval was (4.11,4.31)kPa.The success rate of segment S5 was higher than that of segment S4 (100% [243/ 243] vs 79.83% [194/243]),and the mean value of LSM in segment S5 was lower than that in segment S4 (P<0.05).The mean value of LSM increased with age,and the boys' was a slightly higher than the girls',but there were no statistical differences between them (all P> 0.05).LSM had good consistency in different operators,the intraclass correlation coefficient was 0.92.Conclusion SWE could be an objective and quantitative assessment in liver stiffness of children.
10.Rapamycin inhibits activation of STAT3 in renal tissue of IgA nephropathy rats
Zanhua RONG ; Huijun DUAN ; Jun HAO ; Chen WANG ; Yonghong SHI
Basic & Clinical Medicine 2006;0(05):-
Objective To investigate the effects of rapamycin on p-JAK2,STAT3,p-STAT3 and PCNA in ranal tissue of IgAN rats.Methods The animal models of IgA nephropathy were devided into control group,IgAN model group,losartan group and rapamycin group.The clinical efficacy of rapamycin,and its influences on the protein and mRNA expressions of STAT3,p-STAT3,p-JAK2 and PCNA were determined by western blot,RT-PCR and immunohistochemical techniques respectively.Results The protein and mRNA expressions of p-STAT3,STAT3,p-JAK2 and PCNA were significantly increased in kidney of IgAN rats(P