This study was aimed to investigate potential biomarkers in different traditional Chinese medicine (TCM) syndromes of angina pectoris of coronary heart disease (CHD) by using metabolomic technology,and to explore the objective law of different TCM syndromes of CHD.Endogenous metabolites in serum and urine from the healthy group,and patients with DHD angina pectoris patients of the syndrome of Qi deficiency and blood stasis,as well as the syndrome of Qi deficiency,blood stasis and phlegm turbidity were detected by the liquid chromatography-mass spectrometry (LC-MS).Metabolic profiles were analyzed by the principal component analysis (PCA) and the orthogonal partial least squares discriminant analysis (OPLS-DA).The results showed that in PCA,the healthy group,Qi deficiency and blood stasis group,as well as the Qi deficiency,blood stasis and phlegm turbidity group can be obviously distinguished.Potential biomarkers in the Qi deficiency and blood stasis syndrome contained aspartyl methionine and cysteine sulfinic acid.Potential biomarkers in the Qi deficiency,blood stasis and phlegm turbidity syndrome contained hippuric acid,amino glucose,fructosamine and triglyceride.The objective performance of Qi deficiency syndrome was the absence of biotin,lysyl tyrosine,phosphatidylglycerol and glycocholic acid.It was concluded that through the new metabolomic technology,different endogenous metabolites in the syndrome of Qi deficiency and blood stasis,as well as the syndrome of Qi deficiency,blood stasis and phlegm turbidity of patients with DHD angina pectoris were detected.It provided ideas for the clinical practice of prevention,diagnosis and treatment of different TCM syndromes.

Objective There are many interferences such as power interference, electrode polarization interference, myoelectricity interference, baseline drift, apparatus buzz and so on in electrocardiosignal acquisition system. It is very difficult to effectively remove these interferences in the process of actual measurement. Methods Power harmonic interference, electrode polarization interference and myoelectricity interference were removed by low-pass filter. Baseline drift was removed by high-pass filter. Power basic frequency interference was removed by 50 Hz trap filter. Results These three design proposals had such advantages as good effect, rapid speed and easy realization. Conclusion They can be well applied in ECG monitor with embedded CPU and real time character. Besides, they can also be used in medical apparatus measuring other biological electric signals and industrial observation and control system.

Objective To explore the expression of SLAM gene in the CD4+ and CD8+ T cells from SLE patients.Method The CD4+ and CD8+ T cells from peripheral blood of 20 SLE patients and 10 healthy blood donors were separated using magnetic cell sorting system(MACS).We detected the expression of SLAM mRNA in CD4+ and CD8+ T cells by RT-PCR.Results The CD4+ and CD8+ T cells from SLE patients showed significantly higher SLAM expression than those from healthy blood donors.Conclusion The SLAM signal pathway may play an important role in contributing to the abnormal activation of T cells in SLE patients.

Objective: To prepare silibinin solid dispersion and measure its dissolution in vitro .Methods: Silibinin solid dispersions were obtained with urea, PVP and poloxamer188 as carriers by melting and coevaporation methods. Differential thermal analysis and powder X ray diffraction were used to determine the status of drug in carriers, and the dissolution characteristics in vitro were studied in simulated gastric juice. Results: In PVP silibinin solid dispersions drug was amorphous; in poloxamer188 silibinin solid dispersions, drug existed as fine crystal, while in urea silibinin solid dispersions most of silibinin existed as crystal, only a little as molecule. Poloxamer188 was the better carrier in improving the solution and dissolution rate of the drug. Conclusion: Poloxamer188 is a very useful carrier in improving the solubility and dissolution of silibinin. [

Object To study the ultrastructure destruction of plants in the process of microwave assisted solvent extracion and its mechanism.Methods Cell structures of fresh leaves of Ginkgo biloba L. were observed by transmission electron microscpoe.Results The cell structure changed when the leaves were under microwave radiation or soaked by solvents or heated, such as plasmoysis, destruction of cell organellaes, disappearance of starch grain and so on, while the cell wall was not broken both in microwave assisted solvent extraction and in traditional heating extraction.Conclusion Microwave radiation could lead to the relaxation of the cell structures, but not be enough to make the cell wall broken.

Objective To establish a noninvasive method for prenatal genetic analysis by using maternal serum and apply the method in fetal sex determination,paternity testing. Methods Samples of maternal serum from 53 pregnant women (11 to 36 weeks of gestation) were collected. The DNA extracted from each sample was amplified by using"Y-PLEX 6" amplification kit .which enabled the simultaneous analysis of six Y-STR loci including DYS393.DYS19.DYS389 II, DYS390, DYS391 and DYS385. The PCR products were detected by using ABI PrismTM 377 Sequencer and genotyped by related analysis software. Results (1) Y-STR specific alleles were detected in the maternal sera of all 29 mothers bearing male babies. Among the six Y-STR loci,specific alleles were detected in 29/29 at DYS393 locus,in 18/29 at DYS19 locus and in 10/29 at DYS390 locus. (2) Y-STR specific alleles were not detected in maternal sera of 24 pregnant women bearing female babies. (3) According to the presence of specific alleles at DYS393 locus and the value of allelic peak height and peak area, the accuracy of fetal sex determination was 100% . (4)The observed Y-STR alleles of each prenatal specimen from pregnant women with male fetuses were the same as the results of their husbands. Conclusion The assay of highly polymorphic Y-STR genotyping system developed by the authors provided a sensitive, accurate and non-invasive method to prenatal diagnosis. Our results demonstrate that fetal sex can be accurately determined and imply that paternity testing could be performed for pregnant women carrying male fetuses.

Objective To investigate the abnormality of CD4 and CD8 subsets of T lymphocytes in the peripheral blood of systemic lupus erythematosus patients. Methods The CD4 + and CD8 + T lymphocytes from 20 SLE patients and 10 healthy controls were separated using magnetic activated cell sorting (MACS). Results The numbers of CD4 + T cells in the peripheral blood and CD4 +/CD8 + ratio of SLE patients were significantly lower than those in healthy volunteers(CD4,P=0.001;CD4/CD8 ratio,P=0.046). Conclusion There exist abnormalities in the CD4 and CD8 subsets of T lymphocytes in the peripheral blood of SLE,especially the decreased numbers of CD4 + T cells.

OBJECTIVE:To establish a HPLC method for the content determination of testosterone propionate in testosterone propionate paints.METHODS:The assaying was conducted on a Kromasil C 18 column with methanol-water solution(80∶20)as mobile phase,the detection wavelength was254nm and the flow rate was1.0ml/min.RESULTS:The linear concentration range of the testosterone propionate was100～400mg/ml(r=0.9997)with average recovery rate at98.5%(RSD=0.55%).CONCLUSION:The method is simple,accurate and reproducible,and it can be used for the quality control of testosterone propionate paints.

OBJECTIVE: To investigate the effect of poloxamer 188 as co-emulsifier on the pharmaceutical property of emulsion.METHODS: Diclofenac sodium was used as model drug and poloxamer 188 as co-emulsifier,the emulsion was prepared by high pressure homogen-colostrum pH adjusting method,with its entrapment efficiency,particle size,?-electric potential etc determined.RESULTS: The emulsion which was added with poloxamer 188 showed decreased entrapment efficiency and ?-electric potential but increased particle size as comapred with the one without poloxamer 188.CONCLUSION: The pharmaceutical property of emulsion wouldn't necessarily be enhanced by adding poloxamer 188 as co-emulsifier.

Objective To investigate the effect of photodynamic therapy with chlorin e6 (Ce6) and 5-aminolevulinic acid (5-ALA) on mouse model of malignant melanoma. Methods The mouse model of malignant melanoma was established by injecting 0.1 ml A-375 cells (about 2?10 6 cells) under the right hind leg of BALB/c-6 mice,and that the yellowish white node appears at injection site proves the successful model. Twenty-four of 27 successful mouse models were irradiated at the tumor site with semiconductor laser (wavelength 652 nm) with a total dose of 100 J/cm 2 . Before laser exposure,the mice were treated with 10% 5-ALA by topical compress for 2 h or 7.5 mg/kg Ce6 by intraperitoneal injection for 1 hour or 5-ALA topical application combined with intraperitoneal injection of Ce6 (n=6 in each group). Another six mice as control only underwent PDT. One week after PDT,the mice were killed,the tumor mass was peeled off and weighed,whether the metastasis occurred or not was detected,and the tumor,liver,spleen,lung,kidney were sent to histopathological examination. Results The tumor weight in 5-ALA group,Ce6 group,and the combined group had significant difference as compared with control group (P0.05). The dehydration and scab formation and necrosis could be seen in tumor sites at 1 week after PDT. The cell collapse and necrosis,subdermal thrombosis and cell outline clouding could be observed by histopathological examination. Metastasis of melanoma were found in 5-ALA group,Ce6 group,and the combined group. Conclusion PDT with Ce6 and 5-ALA could kill the malignant melanoma effectively in animal experiment but could not affect the metastasis of melanoma.