Objective To investigated the efficacy of minimally invasive technique combined with anterolateral thigh flap in repairing skin and soft tissue defects of leg and foot.Methods Anterior thigh flap was used to repair 20 cases of skin and soft tissue defects of leg and foot,the lesions defect area was 3.9 cm × 7.4cm-11.4 cm × 20.6 cm.Given the appropriate minimally invasive techniquesurgical treatment,the effect of follow-up was observed after operation.Results The supply valve area healed.The postoperative flaps of 20cases of patients all survived,no vascular crisis occurred.The color of skin flap was normal,close to normal skin color,and the elasticity of the skin was good,no crack,no fat,no pigmentation and other complications occurred,and no effect on shoes and ankle joint activities.Excellent and good rate of overall assessment flap was 90％(excellent in 12 cases,good in 6 cases);excellent and good rate of Mazur ankle function evaluation was 95％(excellent in 12 cases,good in 7 cases).Conclusion The minimally invasive technique combined with anterolateral thigh flap is a more ideal method to repair the leg and foot soft tissue defects.

Objective:To observe the influence of ischemia/reperfusion(simulated by anoxia) on the characteristics of K~(+) channels in renal tubular epithelial cells.Methods: Healthy renal tubule epithelial cells were divided into normal control group(cultured in serum-free RPMI 1640 medium),anoxic group(treated with antimycin A + serum-free RPMI 1640 medium),and oxidative stress group(H_(2)O_(2)+ serum-free RPMI 1640 medium)(n=15).The whole-cell patch clamp technique was used to examine the characteristics of K~(+) channels in renal tubular epithelial cells.Results: The transmembrane K~(+) current in tubular epithelial cells was voltage-dependent and could be blocked by K~(+) channel blocker Ba~(2+).The transmembrane potential levels in anoxic group and oxidative stress group were remarkably higher than that in normal control group(P

Objective To discuss the clinical manifestation and diagnosis of lymphomatous infiltration of the lung.Methods Clinical informations of 17 cases of lymphomatous infiltration of the lung were assessed.Results of 78 patients with malignant lymphoma,17 patients were infiltrated in lung.3 cases were Hodgkin's disease,14 cases were non -Hodgkin's lymphoma.All cases were advanced patients.Survival time of 1 patient was more than 5 years.Survival time of 1 patient which was the shortest was 2 months.Conclusion Patients with lymphomatous infiltration of the lung are not rare.The clinical symptoms and signs are not typical,image diagnosis is the main part in diagnosis of lymphomatous infil- tration of the lung.

As the most popular simplified model of the optical imaging system, the acquisition of the Gaussian point spread function (PSF) parameter is one of the hotspots and key points on which people do research in the field of image restoration. Based on the idea by which there exists deterministic mathematical relationship between Gaussian OTF feature points as well as its parameter and the frequency representation of the image in an existed literature, we proposed an automatic, accurate, stable, and improved approach. This method is able to give prominence to the related calculation feature by a Gaussian convolution and degeneration operation and finally realize the automatic esti mation of PSF parameter of a microscopic image. Experiments have proved that a good restoration result can be achieved utilizing the estimated PSF by the present method, which is of considerable application and reference value in restoration of other sorts with Gaussian approximate PSF model or 3D microscopic image restoration.
Algorithms ; Humans ; Image Interpretation, Computer-Assisted ; Imaging, Three-Dimensional ; Microscopy ; Models, Theoretical ; Normal Distribution

Stromal cell-derived factor-1(SDF-1)is an early cell growth factor which belongs to a subfamily of chemokines.The CXCR4 is SDF-1 receptor.The SDF-1 interacting with CXCR4 plays important roles in physiological and pathological processes such as hematopiesis, stem cell homing,angiogenesis and leukemic cells infiltrating.To study the expression of CXCR4 in leukemia and the relationship between CXCR4 and leukemic cells infiltration can add indicators for leukemic MIC diagnosis of immunology indexes and have great significance to take molecule targeted therapy for preventing leukemia from infiltration or relapse as well.

0.05)and the treatment costs were 1 104.60 yuan,2 049.24 yuan and 3 247.99 yuan,respectively(P

Image interpolation is often required during medical image processing and analysis. Although interpolation method based on Gaussian radial basis function (GRBF) has high precision, the long calculation time still limits its application in field of image interpolation. To overcome this problem, a method of two-dimensional and three-dimensional medical image GRBF interpolation based on computing unified device architecture (CUDA) is proposed in this paper. According to single instruction multiple threads (SIMT) executive model of CUDA, various optimizing measures such as coalesced access and shared memory are adopted in this study. To eliminate the edge distortion of image interpolation, natural suture algorithm is utilized in overlapping regions while adopting data space strategy of separating 2D images into blocks or dividing 3D images into sub-volumes. Keeping a high interpolation precision, the 2D and 3D medical image GRBF interpolation achieved great acceleration in each basic computing step. The experiments showed that the operative efficiency of image GRBF interpolation based on CUDA platform was obviously improved compared with CPU calculation. The present method is of a considerable reference value in the application field of image interpolation.
Algorithms ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Normal Distribution ; Software

BACKGROUND:Bionic structure is one of the most important goals of scaffold design in tissue engineering. However, the majority of the bionic scaffolds do not have the capacity of sustained release of growth factors.
OBJECTIVE:To fabricate a novel biomimetic nanofibrous heparin/gelatin scaffold and investigate the characterization of the scaffolds including the microstructure, the mechanical strength andin vitro recombinant human bone morphogenetic protein-2 (rhBMP-2) release.
METHODS:Heparin-conjugated gelatin was prepared by chemical crosslinking and mixed with gelatin at different ratios. Nanofibrous composite scaffolds with different ratios of heparin-conjugated gelatin (0, 20% and 40%) were fabricated by using a thermaly induced phase separation combined with particle leaching technique. The amount of conjugated heparin in the scaffolds, scaffold microstructures and mechanical strength were detected and observed. The release kinetics of rhBMP-2 from the scaffolds with different concentrations of heparin conjugated gelatin was detected using ELISA method.
RESULTS AND CONCLUSION:The composite scaffolds possessed abundant porosities, good mechanical properties, and biomimetic nano-structures. The macroporous size was 250-420 μm. When the ratio of heparin-conjugated gelatin was 0, 20% and 40%, the content of heparin in the scaffolds was 0, (12.82±2.49) and (25.08±3.69) mg/g, respectively, with significant differences; the elastic modulus of the composite scaffolds was (299±38), (286±40) and (236±33) kPa, respectively, with insignificant differences (P > 0.05). With the increase in the content of heparin-conjugated gelatin, the rhBMP-2 adsorption capacity of the nanofibrous heparin/gelatin scaffold is improved, thereby significantly reducing the burst release of rhBMP-2 and prolonging the releasing time.

OBJECTIVE:To investigate the characteristics and trends of illegal circulation of counterfeit drugs in China,and to put forward monitoring countermeasures. METHODS:In empirical study,3 typical cases of counterfeit drugs manufacturing and selling in China were analyzed (including Jiangsu Wuxi Internet transnational counterfeit drugs manufacturing and selling case, Shanghai Avastin eye drug case,Sichuan Dazhou counterfeit drugs case),to compare the sources of counterfeit drug and illegal cir-culation pathway,thus find out the monitoring problems. RESULTS & CONCLUSIONS:Main reasons for illegal circulation of counterfeit drugs in China are as follows as drug circulation regulation absence of foreign-funded medical institutions,the existence of hidden drug circulation monitoring holes in urban medical institutions,under-developed and weak ability of distinguishing talse drug information internet counterfeit drugs sales regulatory means. It is suggested to establish drug management norms for for-eign-funded medical institutions,improve drug circulation monitoring in primary medical institutions,establish pharmaceutical logis-tics(transportation)licensing system and strengthen online fake drug sale information monitoring and information publication.

OBJECTIVE:To explore consumers’perceptions for pharmaceutical care by community pharmacist when purchas-ing OTC drugs. METHODS:Semi-structured interview was conducted for 100 consumers who were recruited in Xingqing District, Jinfeng District and Xixia District of Yinchuan,Ningxia Hui Autonomous Region,their expection for pharmaceutical care by com-munity pharmacists was summarized. RESULTS:Totally 100 residents were interriewed,and 97 completed a full interview with ef-fective interview rate of 97%. Consumers still did not clearly on distinguishing the OTC drugs. The majority of consumers chosen OTC drugs were based on experience and brand,a handful of consumers were under the guidance of community pharmacists. Con-sumers were most concerned about the efficacy,adverse reactions and drug quality in using OTC drugs. Most consumers were not satisfied with the pharmaceutical care in purchasing OTC drugs. Nevertheless,due to a lack of medicine knowledge,consumer had a great demand for pharmaceutical care and willingness to accept it. Low professional level of community pharmacist and staff in current community pharmacy and lack of effective communication between pharmacist and consumer were the obstacles of develop-ing pharmaceutical care. CONCLUSIONS:Because of their distrust on competence of community pharmacist,consumers seek less pharmaceutical care of OTC drugs at community pharmacy though they had expectations on pharmaceutical care of OTC drugs by community pharmacist. Consumers’rely on self-choice of drugs make them exposed to more drug risks.