The purpose of this work was to reestablish the respiratory abduction of the paralyzed vocal cord through reinnervation of the posterior cricoarytenoid (PCA) muscle by partial phrenic fibres.In fifteen adult cats the adductor branch of the recurrent laryngeal nerve (RLN) of the right side was cut and its distal end ligated, while its proximal end was implanted into the PCA muscle belly.The whole RLN was then transected in the tracheo-esophageal groove and its distal stump anastomosed to the upper branch of the phrenic nerve.Direct laryngoscopy showed that the inspiratory abduction of the paralyzed vocal cord recovered within 40 d in all cats.80 d later, a larger abducent motion of the glottis was observed on the reinnervated side.Abduction was caused by reinnervation of the PCA muscle from phrenic motoneurons, as demonstrated by laryngeal electromyography and histological testings.The function of the diaphragm was preserved as revealed by monitoring of the intrathoracic pressure.

Objective To observe the clinical effect of interferon -1 b (Yundesu)in the treatment of verru-ca planae.Methods 1 1 0 patients with verruca pianae were randomly divided into observation group and control group,55 cases in each group.The observation group was given Yundesu injection 30 g once every day in first week, and once every other day in another three weeks.The control group was given orally mannatide 1 0ml two times every day.The leision changes of the two groups were observed after 4 weeks therapy.Results The total effective rate of the observation group was 92.7%(51 /55),which of the control group was 72.78%(40 /55),the difference between the two groups was statistically significant (χ2 =7.70,P <0.01 ).No adverse reactions in the two groups were observed.Conclusion The clinical effect of Yundesu is more prominently beneficial to patients with verruca planae.

Humans ; Skull Base ; anatomy & histology ; surgery

Recently imageless navigation systems have been developed rapidly, and have become major navigation system in clinic. It comprises computer workstation, infrared optical localizing system, foot control pedal and navigation software. Currently, imageless navigation systems have been used successfully in total knee arthroplasty, uni-compartment arthroplasty, total hip arthroplasty, hip surfacing arthroplasty, total shoulder arthroplasty, anterior cruciate ligament reconstruction and peri-articular osteotomy of knee, etc. The first OrthoPilot imageless navigation system was used by Saragaglia et al in total knee arthroplasty in January 1997, and many clinical studies have been conducted. The clinical results show that this kind of systems greatly improves operative accuracy, and reduce cases beyond ideal range. It shows accuracy as CT-based navigation systems, and they can be commonly used as standard navigation systems in clinic.

Hearing ; Humans ; Neuroma, Acoustic ; surgery ; Neurosurgical Procedures ; methods

China ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Objective:To study the influence of dietary restriction on the expression of insulin signaling pathway genes in mouse adipose tissue. Method:Twenty four C57BL/6 male mice of 2 w age were subjected to either AL (ad libitum) or DR (dietary restriction, 40% from AL) randomly until sacrificed.Then the epididymis fat pads were weighed, and their insulin signaling gene expression was detected by Oligo Insulin Signaling Pathway Microarray (SuperArray), and two of the differentially expressed genes, NPY and AKT3 were validated by Real Time PCR. Results:Dietary restriction significantly reduced the weight of epididymis fat pads and 31 (20 up, 11 down regulation) of 96 insulin signaling pathway genes were differentially expressed. The results of Microarray coincided with that of Real Time PCR. Conclusion:The differential regulation on the expressions of insulin signaling pathway gene may be the important molecular basis of dietary restriction.

Objective: In this study,we evaluatedthe influence of different nutritional support routes and nutrients on the intestinal barrier function and bacterial translocation in rats with gut I/Rinjury. Methods: Eighty rats were divided randomly into: I/R group,control group,and nutrition-supportgroups. Bacterial culture, D-lactate and endotoxin levels was assayedafter 7 days of nutrition support. Results: There was serious damagein intestinal tissue of I/R group. Remarkably higher value of bacterial translocation occurred in PN group (P0.05).The endotoxin level was the highest in PN group. Conclusions: TPN alone could impair gut immune barrier function and thereforefacilitate bacterial translocation.The use of immunonutritionindicates limited efficacyin preventing bacterial translocation compared with common enteral nutrition.

Cochlear Implants ; Magnetic Resonance Imaging ; Positron-Emission Tomography

Objective To evaluate the effect of anisodamine on expression of Toll-like receptor 4 (TLR4) in the kidneys of exhausted rats.Methods Forty-eight male SD rats weighing 200-220 g were randomly divided into 3 groups:control group (group Ⅰ ) (.n =8) ; exhaustion group (group Ⅱ ) ( n =24) and anisodamine group (gronp Ⅲ) (n =16).In groups Ⅱ and Ⅲ exhaustion was induced by exhausting swimming and defined by (1) the animal sank to the bottom and stayed for＞ 10 s during swimming,(2) uncoordinated swimming,and (3) the animal lost escape/righting reflex when placed on the table In group Ⅲ intraperitoneal anisodamine 10 mg/kg was administered at 20 min before swimming.The animals were sacrificed immediately (T1 ),6 h (T2 ) and 24 h (T3 ) after exhaustion respectively.Their kidneys were obtained for detection of apoptosis and determination of TLR4 protein (by immuno-histochemical staining) and mRNA (by RT-PCR).Results Exhaustion significantly increased the number of apoptotic cells and up-regulated TLR4 protein and mRNA expression in group Ⅱ as compared with group Ⅰ,and anisodamine significantly attenuated the exhaustion-induced increase in the number of apoptotic cells and up-regulation of TLR4 protein and mRNA expression in group Ⅲ as compared with group Ⅱ.Conclusion Anisodamine can decrease apoptosis in renal cells in exhausted rats bydown-regulating TLR4 expression.