The ghrelin is an endogenous ligand of the growth hormone (GH) secretagogue receptor,which can regulate the secretion of GH,stimulate the appetite,adjust the energy metabolism,and improve the cardiovascular function.Children with congenital heart disease (CHD) usually suffered from malnutrition and growth retardation,which were connected with insufficiency of energy intake,poor absorption,and increase of nutritional requirement,anatomy and the changes of hemodynamics.Many infants with CHD gradually present ventricular dysfunction and growth restriction in their early childhood,which consequently lead to severe complications.Ghrelin is valuable in the early detection of potential CHD,plays an important role in improving the state of malnutrition and protecting the cardiac function,and provides an important reference to explore the new drug therapy for CHD.This review summarized the effects and significances of ghrelin on growth and cardiac function in infants with CHD.

Objective] To study the transmission of the Ishinpo versions ,understand the prevailing version of Ishinpo. [Method] With the research methods of the Chinese traditional history and philology,collect and sort historical cited references of the Ishinpo, by paying attention to three main emendation in the transmission history of Ishinpo. [Result] Ishinpo of Nakarai is the golden version for document research. The prevailing Ansei Ishinpo version of contemporary China is based on Nakarai version,which consists of twenty seven scrolls of Hei era and three scrolls of Edo Era. Ishinpo Versions have been divided into Emperor Nakarai versions,Uji versions and Family tradition versions. There are big differences in utilization and completeness of the three version system. The Emperor Nakarai versions are relatively good, of which the Nakarai version preserves best. The Uji versions are widespread, of which the RenHe Temple version is better preserved but lacuna. There are lots of lacunas in the family tradition versions, owing to circulateed copies. [Conclusion] In the three main emendation, of Ishinpo, mostly, the Nakarai version is the source edition, the Uji version is primary edition, and the family tradition version is the secondary edition. The paper, which is useful in the transmission of the Ishinpo and thus historical process of medical exchange between China and Japan, has established the system of Ishinpo versions, defined the golden version and the prevailing version.

What the medical book reviews focus are the academic contents and update information in medical books, and the popularization of update scientific achievements. The reviewed medical books must have the value for handing down scientific achievements and face the new problems and challenges encountered in the development of medical sciences. Medical book reviews are the booster for the accumulation and development of medical knowledge in medical college and university library. The book reviews written by medical experts were summarized,the experiences with the development of medical book review resources were described, and the advertising of network platform for book reviews was elaborated in our library.

Autophagy is a powerful process for removing such proteins and for maintaining homeostasis.However,autophagy dysfunction has also been implicated in the pathogenesis of vari-ous neurodegenerative diseases,including Parkinson 's disease (PD).Recent studies have shown that TFEB could regulate au-tophagy and lysosome function through regulating the expressionof the relatedgenes.Thus,TFEB plays a key role in the occur-rence of Parkinson's disease.Therefore,this article will make a review of the regulatory mechanism of TFEB and its role in Par-kinson's disease.

Objective To analysis the clinical efifcacy of blue-ray combined with Yinzhihuang Keli for newborn pathological jaundice. Method 120 newborns with neonatal jaundice were randomly divided into control group(traditional treatment group, took enzyme inducers phenobarbital and continuous blue-ray radiation for 8 h everyday)60 cases and treatment group(Yinzhihuang Keli combined with traditional treatment group)60 cases,7 days for a course of treatment , then the efifcacy of two groups were compared. Results After 7 days treatment, we found compared with control group, the speed of decline of serum bilirubin of treatment group was much faster, the degree of decline was much bigger and the average time of jaundice subside was much shorter, all P<0.05.The total efifciency of treatment group was 98.33%which was signiifcantly higher than the control group which was 91.67%, P<0.05.Conclusion Compared with conventional treatment methods, blue-ray combined with Yinzhihuang Keli for neonatal jaundice would get a better efifcacy, faster speed of jaundice subside and smaller side effects.

Objective:The present research aimed to explore the involvement of ERK signaling pathway in apoptosis of hepatic carcinoma induced by decitabine combined sodium valproate.Methods: HepG2 cell line was incubated with decitabine combined sodium valproate and the inhibition rate was detected by MTT method.The apoptotic related protein and ERK signal pathway proteins were assayed by Real-time PCR or Western blot.Results: The HepG2 cell line was inhibited greatly by decitabine combined sodium valproate medication with an increased expression of Caspase3,Caspase9 and Bax (P<0.05).The expression of Ras,Raf,MEK and ERK1/2 was increased dramatically after incubation with decitabine combined sodium valproate when compared with control group ( P<0.05).Conclusion:Decitabine combined sodium valproate exert a significant inhibition on the proliferation of HepG2 cell line by nor-malizing the abnormal MEK/ERK signaling pathway.

Objective: To detect the correlation of lymphatic vessel density (LVD) with a high mobility group box-1 Protein (HMGB1) and tumor-associated macrophages (TAMs) in cervical carcinoma and the effect on prognosis. Methods:Immunohistochem-istry was applied to detect HMGB1, CD68, and D2-40 expressions in cervical squamous cell carcinoma in 93 cases. t test,χ2 test, Spear-man rank correlation analysis, Kaplan-Meier method, and Cox regression were performed to analyze the expression levels, correlation, and prognosis. Results: HMGB1 protein, CD68, and D2-40 were highly expressed in CSCC. As HMGB1 and TAM expressions in-creased, lymphatic vessel density increased. As HMGB1 and TAM expressions decreased, lymphatic vessel density decreased. Positive correlations were also found between the HMGB1 protein, TAM content, and LVD. In the group with low HMGB1 and TAM expres-sions, the survival time of the group with a high LVD expression was significantly lower than that of the group with a low LVD expres-sion. A multivariate Cox regression model showed that HMGB1 and lymph node metastasis were independent prognostic factors. TAMs and LVD were not independent prognostic factors. Conclusion:HMGB1 proteins and TAMs were highly expressed in CSCC. Patients who exhibit increased HMGB1 expression or increased TAM count consequently show enhanced LVD expressions, increased lymph node metastasis, and poor prognosis.

Objective To explore the changes of T-lymphocyte subsets,D-Dimer and immunoglobulin in mycoplasma pneumonia children and its significance.Methods The levels of T lymphocyte subsets (CD3 +、CD4 +、CD8 +、CD4 +/CD8 +)in 50 children with mycoplasma pneumonia (MPP Group)and 50 healthy children were detected by flow cytometry;The levels of D-Dimer were detected by immunoturbidimetry;The levels of serum immunoglobulin were detected by immune turbidimetric method.Results The results of T lymphocyte subsets:Compared with normal control group, CD3 +and CD4 +peripheral blood cells in MPP group were significantly declined(P<0.001)、CD8 +cells were increased slightly,but with no significant difference、CD4 +/CD8 +ratios were decreased slightly.The results of D-Dimer:Compared with normal control group,the levels of D-Dimer in MPP group were significantly escalated(P<0.001).The results of immunoglobulin:IgA and IgMin MPP group were both significantly higher than those of normal group,but IgG was not different from that in normal group.Conclusion Children with mycoplasma pneumonia have immune disorder,which may generate abnormal clotting.Therefore,besides the conventional anti-mycoplasma pneumonia treatment,we need to detect the levels of D-Dimer according to the clinical symptoms and disease progression and strength the regulation and treatment of the immune system.

Objective To study the T Lymphocyte Subsets and the cytokines in the newborns with infectious diseases. Methods Twenty cases of neonatal bacterial pneumonia (bacterial group), 15 cases of rotavirus enteritis (virus group) and 20 newborns with jaundice (control group) were recruited in this study. The peripheral CD4+T Cells and lymphocyte subsets were assessed by flow cytometry. Results The IL-4 level was significantly different among different groups (F=3.39, P=0.041). The levels of IL-17 and IFN-γdid not differ signiifcantly among different groups (F=0.28 and 1.24 respectively, P>0.05). The IL-4 level was higher in bacterial group than that in virus group and control group (P<0.05). The percentages of CD3+, CD4+, CD19+and NK cells and the ratio of CD4+/CD8+ had significant difference among different groups (F=3.30-26.69, P<0.05). The percentages of CD3+and CD4+cells and the ratio of CD4+/CD8+were lower in bacterial group and virus group than those in control group (P<0.05). The percentage of CD19+cells was higher in bacterial group and virus group than that in control group (P<0.05). The percentage of NK cells was lower in virus group than that in bacterial group and control group (P<0.05). Conclusions The pattern of cytokines level is different in newborns with infection caused by different pathogens. Newborns with infectious diseases have immune dysfunction and Th2-dominated imbalance. The low percentages of T lymphocyte subsets indicate the depressed cellular immunity after infection, which may result in atypical symptom and prolonged disease course.

Objective To establish the L929 cell line with high expression of asporin ,and to study the effect of asporin on the expressions of TGF- β1 and Col2α1 in mouse fibroblast L929. Methods Asporin cDNA was amplified from mouse mandible tissue by RT-PCR,then was inserted into the eukaryotic expression plasmid pcDNA3.1(-). The recombinant plasmid pcDNA3.1(-)-ASPNIII was transfected into the mouse fibroblast cell line L929, followed by G418 treatment. RT-PCR and Western blot assays were used to screen the stable, asporin high expressing L929 cell line.The effect of overexpression of asporin on the regulations of TGF-β1 and Col2α1 was further studied. Results The stable, aspori high expressing n L929 cell linewas successfully established. Results of RT-PCR and Western blot assays showed that overexpression of asporin could effectively inhibit the expressions of TGF-β1 and Col2α1. Conclusion Enforced expression of asporin can inhibit the expression of TGF-β1 and Col2α1 in L929 cells.