Objective To evaluate CT manifestations and pathologic findings of thyroglossal cyst.Methods CT manifestations,clinical materials and pathological findings of 12 cases with thyroglossal defined by pathology were analysed.Results Of 12 cases,the the lesions were located at the center line of the neck in 8 cases and at left of the neck in 4 cases.The cysts were round or oblate in shape on CT scans.In 5 cases the walls of cyst were smooth and no enhanced;while in other 7 cases the cystic walls were coarse and enhanced evidently which there were infiltration of inflammatory cell microscopic examination.The nodules at cystic wall which were composed by blood vessel,striated muscle and connective tissue on pathology could be seen on CT scans while if these nodules were minor,they could hardly to be showed by CT.Conclusion CT can make a proper diagnosis of thyroglossal cyst.

Objective To investigate a method for the purification of the N?terminal peptide fragment(NT)of the myocardial calcium channel Cav1.2,and characterize its interaction with calmodulin(CaM). Methods EscherichiacoliBL?21 cells were transformed with plasmid pGEX?6p?3/NT harboring the NT?GST fusion gene. The cells harboring pGEX?6p?3/NT were cultured and protein expression was induced with isopropyl?β?D?thiogalactoside(IPTG). Then,the GST?NT fusion protein was purified by using glutathione Sepharose 4B(GS?4B)beads. GST was cleaved off with the PreScission protease,and SDS?PAGE was performed to detect the purity and relative molecular weight of the purified peptide. Further, GST pull?down assay was performed to characterize the interaction of the NT peptide with CaM. Results SDS?PAGE analysis showed that the NT peptide was successfully purified,with high purity. Results of the GST pull?down assay showed that the NT peptide could interact with CaM. Conclusion This study establishes a method for the purification of the NT peptide and lays the foundation for further research on the interaction partners and biological functions of NT.