Objective To investigate the significance of expression of peroxisome proliferator activated receptor ?(PPAR?) and retinoid X receptor ?(RXR?) in chronic gastritis, gastric mucosal dysplasia and gastric carcinoma and to identify the correlation between PPAR? and RXR?. Methods Avidin biotin peroxidase complex immunohistochemical methods were adopted to examine the expression of PPAR? and RXR? in 53 patients with gastric carcinoma, and 18 with gastric mucosal dysplasia, 31 with chronic non atrophic gastritis and 30 with chronic atrophic gastritis were served as controls. Results The positive rates of PPAR? and RXR? were 41.5% and 54.7% in gastric carcinoma respectively, 27.8% and 38.9% in gastric mucosal dysplasia, 10.0% and 20.0% in chronic atrophic gastritis, 6.5% and 16.1% in chronic non atrophic gastritis. From chronic non atrophic gastritis, chronic atrophic gastritis to gastric carcinoma, expressions of PPAR? and RXR? showed an ascending tendency. Compared with those in chronic gastritis, expressions of PPAR? and RXR? in gastric mucosal dysplasia and gastric carcinoma were significantly enhanced ( P0.05). There was a significant correlation between expressions of PPAR? and RXR? in gastric carcinoma ( r =0.54). Conclusion PPAR? and RXR? protein overexpression is a relatively early event in gastric carcinogenesis, and it may play both an independent and synergetic role in progression of gastric carcionma.

Objective To compare the effect of seven commonly used nonsteroidal anti inflammatory drugs (NSAIDs) on proliferation, apoptosis, and neoplasmagenesis of gastric cancer cells in vivo. Methods Gastric cancer cell lines were treated with NSAIDs (aspirin 0-400 ?mol/L, indomethacin 0-25 ?mol/L, and ibuprofen, naproxen, sulindac, nimesulide, celecoxib 0-200 ?mol/L, respectively). Proliferation of the cells was detected by using MTT assay. Apoptosis of cells was measured by using fluorescence activated cell sorter (FACS). Nude mice bearing gastric cancer xenografts were administrated with NSAIDs (indomethacin 3 mg/kg, sulindac 8 mg/kg, nimesulide 6 mg/kg, celecoxib 15 mg/kg) for 30 days, and then the weight of implanted tumors was measured. Results There was a dose dependent inhibition of cell proliferation by majority of NSAIDs used, celecoxib the most, except for ibuprofen and naproxen. In nude mice, NSAIDs also showed a suppressive effect on tumor growth with inhibitory rate of celecoxib as (93.8?0.97)%, nimesulide (93.1?1.78)%, indomethacin (89.9?5.61)% and sulindac (89.3 ? 2.07)%. Once incubated with celecoxib and indomethacin, the gastric cancer cells went to apoptosis with an increase in percentage of apoptotic cells up to 30.4% and 23.9%, respectively. Conclusions Many NSAIDs, celecoxib in particular, appeared to be suppressive to gastric cancer cells with exception for ibuprofen and naproxen. Induction of apoptosis might be one of the mechanisms that NSAIDs inhibit gastric cancer.

The aims of this study were to compare the inhibitory effect of nimesulide and indomethacin on gastric cancer cells, to investigate the effect of nimesulide on SGC7901 and 7901-AS, and to evaluate its probable mechanism. After incubated with nimesulide (0～200?mol/L) or indomethacin (0～25?mol/L), the proliferation of gastric cancer cells was measured by MTT assay. The cell cycle of SGC7901, 7901-P, 7901-AS was respectively observed after being incubated with nimesulide and vehicle control, by using fluorescence activated cell sorter (FACS). Ultramicrostructure of gastric cancer cells (SGC7901,7901-AS,SGC7901+nimesulide) was observed by electronmicroscopy. The results showed that by MTT assay nimesulide, as indomethacin, had dose-dependent inhibitory effects on proliferation of gastric cancer cells. Compared to the inhibitory effect of nimesulide on SGC7901, it was less on 7901-AS (P

In this series, 24 cases of ulcerative colitis and 23 cases of chronic colitis were treated with drug partition -moxibustion with total effective rates of 95. 83% and 95. 55% respectively, markedly excelled that of control group treated with western medicine. Comparison made on the results of the 15 cases of ulcerative colitis before and after treatment showed that drug partition - moxibustion inhibits humoral immunity and strengthens cellular immunity. It is suggested that the mechanism might lie on the adjustment on the abnormal ratio between T - lymphocytes subgroups, hence the self - immune state in the patient can be checked and the mucosal lesion in the intestine corrected.

Objective To identify and quantitate the conjunctival aerobic bacterial flora from healthy people of 3 age groups in Chongqing city.Methods Samples taken with moist swabs from the lower fornix of both eyes of 33 children(9.85?0.57 years old),31 youths(21.23?0.88 years old)and 30 elders(72.97?4.94 years old)were given bacterial cultures.Positive ones were identified and colony forming units(CFUs)were counted.Bacterial quantity was expressed as the number of CFUs divided by the weight of each sample measured before and after collection.Results The elders showed a higher isolation frequency(93.33%)than children(45.45%)and youths(50.00%)which had statistically difference(P

The purpose was to investigate the research achievements over the past two decades on moxa smoke produced in the process of moxibustion, in terms of its clinical studies, chemical compositions, safety assessment as well as the mild smoke or smoke-free moxibustion, for revealing the recent dynamic and developing orientation, and promoting its further application, succession and innovation.

Objective To observe the therapeutic effects and action mechanism of moxibustion in the treatment of intestinal fibrosis in ulcerative colitis. Methods Tianshu (ST 25) and Qihai ( CV 6) were the main acupoint; other points were used according to syndrome differentiation. Sixty-five patients were randomized into two groups: Group A in which 32 cases were treated by herbpartitioned moxibustion and group B in which 33 cases were treated by bran-partitioned moxibustion. The therapeutic effects and TGF-β1, Ⅰ and Ⅲ collagen expressions were measured before and after treatment. Results In group A, 17 cases were cured, 12 cases improved and 3 failed; in group B, 1 1 cases were cured, 16 cases improved and 6 cases failed. Ⅰ , Ⅲ collagen expressions were obviously inhibited in the two groups, in group A in particular. Conclusion In the prevention and treatment of intestinal fibrosis, moxibustion may reduce the expression of TGF-β1, and hence to block or inhibit the synthesis of Ⅰ、Ⅲ collagens, and improve the structure and function.

Objective: To investigate the effect of moxibustion on telomerase activity and genes expression in tissues of senescent rats. Methods: Subacute aging rats model were established by injection with D-gal solution. Points Shenshu (BL 23) were treated with moxibustion in treatment group, contrasting with a model group and a normal group. Enzyme-Linked Immunosorbent Assays(ELISA) was used for the level oftelomerase activity in liver tissues, and In Situ Hybridization(ISH) was used for the condition of expression of telomerase genes in liver tissues. Results: The level of telomerase activity in the aging model group was obviously lower than that in normal control group (P＜0.01), the level in moxibustion group was obviously higher than that in model group(P＜0.05). In comparison with normal rats, the positive-expressed areas and photodensity of telomerase genes in aging model group were all significantly lower (P＜0.01,P＜0.01), and the positive-expressed areas in moxibustion group was significantly higher than that in model group (P＜0.01). Conclusions: Moxibustion could regulate telomerase activity of senile rats, hence delaying aging.

Objective To evaluate the feasibility and safety of vaginal enlarged amputation of cervix to treat patients with cervical cancer of stage Ⅰ a1 and cervical intraepithelial neoplasia grade Ⅲ(CIN Ⅲ)who were unfit for conization surgery.Methods From July 2002 to May 2007,patients with cervical cancer at stage Ⅰ a1,diagnosed by pathology after loop electrosurgical excision procedure(LEEP),large area CIN Ⅲ(the area of lesion≥3/4 on colposcopy),CIN Ⅲ coexisted with vaginal intraepithelial neoplasia (VAIN)in the superior segment of vagina,CIN Ⅱ-Ⅲ recurrence or with residual lesion,positive margin after conization of cervix,who wanted to preserve fertility and(or)corpus uteri were selected to receive vaginal enlarged amputation of cervix.Results Forty-eight eases including 5 with cervical cancer in stage Ⅰ a1,38 with large area CIN Ⅲ(9 with gland involvement),2 with residual lesion and 2 with positive margin after LEEP,1 recurrence after cold knife conization,received the procedure successfully.The median age was 34 years(range 27-40),median operation time was 60 minutes(range 30-100),median blood loss was 40 ml(range 5-300),and median hospital stay was 10 days(range 7-17).After follow-up 1-39 months,no patient had postoperative complications and recurrence,and all patients resumed normal menstrual cycle and sexual life.Condusion Vaginal enlarged amputation of cervix appears to be a safe and feasible procedure for patients with cervical cancer at stage Ⅰ a1 and CIN Ⅲ who are unfit for conization surgery.

Asthma is a chronic airway inflammatory disease, and airway remodeling is one of its typical pathological features.Airway remodeling can result in hyperactivity, irreversible airflow limitation, and even decreased lung function.Studies have shown that the Wnt/β-catenin signaling pathway plays an important role in post-injury repair of the lung.Abnormal activation of the Wnt/β-catenin signaling pathway leads to airway remodeling in asthma.This article will review the progress of Wnt/β-catenin signaling pathway and asthma-related airway remodeling, and explore its possible therapeutic targets in asthma.