To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F2a and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.
Anti-Bacterial Agents/*pharmacology ; Dysentery, Bacillary/*microbiology ; Microbial Sensitivity Tests ; Oils, Volatile/*pharmacology ; Shigella flexneri/*drug effects ; Shigella flexneri/isolation & purification ; Shigella sonnei/*drug effects ; Shigella sonnei/isolation & purification

Objective To explore the clinical significance of combined detection of C‐reactive protein (CRP)and white blood cells (WBC)in children with anemopyretic tonsillitis .Methods A total of 284 patients with anemopyretic tonsillitis were divided into ex‐cessive pattern group (131 cases) ,wind‐heat invasion group (153 cases) ,other 120 healthy children were recruited into control group .The WBC ,percentage of neutrophils (NEU % ) ,percentage of lymphocytes (LYM % ) and CRP were analyzed among the three groups .Results WBC and NEU% in excessive pattern group and wind‐heat invasion groups were higher than those in the control group (P< 0 .05) ,CRP in wind‐heat invasion group was higher than that in excessive pattern group and the control group (P< 0 .05) .CRP in the excessive pattern group were higher than that in the control group (P< 0 .05) .When CRP cut‐off value in wind‐heat invasion group was 12 .65 mg/L ,the sensitivity was 90 .6% ,specificity was 86 .3% .Conclusion The combined detection of WBC and CRP have clinical value in differential diagnosis of children with anemopyretic tonsillitis .

To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F2a and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.
Animals ; Anti-Bacterial Agents ; pharmacology ; Dysentery, Bacillary ; microbiology ; Female ; Male ; Mice ; Microbial Sensitivity Tests ; Oils, Volatile ; pharmacology ; Shigella flexneri ; drug effects ; isolation & purification ; Shigella sonnei ; drug effects ; isolation & purification