Object To search for more bioactive alkaloids from Aconitum coreanurn through this fur-ther study. Methods The ethanolic extract from the root of A. coreanum was isolated by aluminium oxidecolumn chromatography, the constituent structures were elucidated mainly by their physicochemical prop-erties and spectroscopic (IR, ESI-MS,1 H-NMR, 13 C-NMR,1 H-1 HCOSY, HMQC, and HMBC)analysis.Results Seven alkaloids were obtained from the root of A. coreanum, they were identified as: 11, 13-di-acetyl-14-hydroxy-2-isobutyryl hetisine ( Ⅰ ), guan-fu base G ( Ⅱ ), guan-fu base F ( Ⅲ ), guan-fu base Z( Ⅳ ), guan-fu base I ( Ⅴ ), guan-fu base K ( Ⅵ ), and guan-fu aminealcohol (Ⅶ). Conclusion Compound I is a new natural product, named as guan-fu base P, its 1 H-NMR and 13 C-NMR data are reported first inthis paper.

Objective To investigate the current application of teaching cadavers and attitudes of medical students towards body donation and anatomy practicum and to make proposals on teaching and learning of human anatomy.Methods A on the spot questionnaire survey among 300 students studying in Hebei Medical University was conducted and the acquired data were contrasted analyzed.Results The teaching cadavers were insufficient and the quality of anatomy education was directly affected.Medical students took unfavorable attitudes towards anatomy practice and knew little about body donation.The main factors affected body donations were traditional concepts,lack of humanistic concern and complicated procedures of donation.Conclusions The short of teaching cadavers would be relieved by enhancing propaganda and legislation,emphasizing humanistic concern,simplifying procedures of donation.By means of anatomy teaching reformations,the learning interest and humanistic literacy of medical students are supposed to be improved.

Objective To compare the traditional anterior transposition and anterior subcutaneous with blood supply of the ulnar nerve for the treatment of cubital tunnel syndrome.Methods Eighty cases with cubital tunnel syndrome were enrolled in the retrospective study from January 2009 to March 2013,40 cases (control group) were treated by traditional anterior transposition while the others (observation group) were treated by anterior subcutaneous with blood supply.According to the evaluation criteria of ulnar nerve,evaluated the function of patients and compared the scores of two groups and the improved rate of different level patients.Results The scores of two groups before surgery were (3.6 ± 0.4),(3.7 ± 0.5) scores,after surgery were (7.5 ± 0.9),(7.4 ± 0.6) scores respectively,there was significant difference between two groups before and after surgery (P ＜ 0.05),but there was no significant difference between two groups (P ＞0.05).The improved rate of observation group was 87.5％ (35/40),which was higher than that of control group [85.0％ (34/40)],but without statistical difference (P ＞ 0.05).In two groups the patients with middle scores showed no statistical significance in improved rate [90.0％ (27/30) vs.96.4％ (27/28)] (P ＞ 0.05).Two treatments showed a significant difference for the low scores patients 8/10 vs.7/12 (P ＜0.05).Conclusion Anterior subcutaneous with blood supply of the ulnar nerve is better than traditional anterior transposition for the gross cubital tunnel syndrome patients.

Objective To study the application effectiveness of healthcare failure modes and effects analysis before the secure transportation of post anesthesia care unit(PACU) patients. Methods A total of 689 general anesthesia post-operative patients who had been recovered in PACU and transported between January to December in 2015 by convenience sampling were divided into 2 groups to receive nursed by traditional method before transportation (contrast group, 346 cases) or nursed both with traditional way and healthcare failure modes and effects analysis method to analyze (observation group, 343 cases). The Medical Risk Priority Number (RPN), Status of Failure modes and satisfaction value of physicians and nurses were compared. Results The RPN value of observation group had been cut down from (229.00 ± 52.91) points to (57.14 ± 16.04) points, there was significant difference (t=7.58, P=0.01). The occurrence rate of failure mode of observation group was 2.62%(9/343), which was obviously lower than 19.36%(67/346) of contrast group, there was significant difference (χ2=49.19, P<0.01). The satisfaction rate of observation has improved significantly from 74.36%(58/78) to 93.59%(73/78), there was significant difference (χ2=10.72, P<0.01).Conclusions Healthcare failure modes and effects analysis management method could find out the failure mode of PACU patient before the secure transportation in time, could decrease the effect of failure mode and would continuously improve the quality of PACU nursing service .

No abstract available.
Cervical Intraepithelial Neoplasia

AIM:To establish the chromatographic fingerprint of Carthamus Tinctorius L.by high performance liquid chromatography(HPLC).METHODS:The chromatographic fingerprint of Carthamus Tinctorius L.was established by HPLC with a RP-C_ 18 column,acetonitrile-water(0.5 phosphoric acid)gradient elution,a DAD diode array detector at 275 nm and the flow rate of 1.0 mL/min,column temperature of 25 ℃。 RESULTS:34 co-peaks of Carthamus Tiuctorius L.were gained from 10 producing provinces.Its basic features were rough consistent each other.Hydrosafflor yellow A,was selected as marker,among samples the content of Xinjiang was the highest in the all samples.CONCLUSION:This method provides useful information for identification of traditional Chinese medicines,the fingerprints of samples of Carthamus Tinctorius L.can be used to control the quality combined with content determination.

We synthesized the superparamagnetic paclitaxel nanoparticles from modified chitosan tangling around Fe3O4 ferrofluid and taxol, and observed the nanoparticles with transmission electronic microscopy (TEM). Then we evaluated the paramagnetism of the particles by vibration specimen magnetometer (VSM) and tested their cytotoxicity with flow cytometry (FCM). The prepared nanoparticle solution was black without any floccule or sediment and appeared transparent after diluted. The nanoparticles were spherical and dispersed in water with mean diameter of 15 nm under TEM and showed superparamagnetic character. FCM test showed the nanoparticles had significant toxic effects against malignant astrocytoma U251 cell lines, equal to taxol alone. These results showed that the superparamagnetic nanoparticle not only enhanced the solubility of paclitaxel in water, but also was superparamagnetic and cytotoxic, which make suitable tools for magnetic targeting chemotherapy of brain gliomas.
Antineoplastic Agents, Phytogenic ; pharmacology ; Apoptosis ; drug effects ; Brain Neoplasms ; pathology ; Cell Line, Tumor ; Chitosan ; pharmacology ; Drug Carriers ; chemistry ; Drug Compounding ; methods ; Ferric Compounds ; Glioma ; pathology ; Humans ; Magnetics ; Metal Nanoparticles ; chemistry ; Nanoparticles ; Paclitaxel ; pharmacology

Objective To grasp the general situation of patent application and authorization of 77 medical and health institutions in Zhejaing,to provide reference and basis for promoting the patent application and conversion.Methods Patent data were searched through Bai Teng net patent database,and SPSS 19.0 was used for statistical analysis.Results The total number of patent application showed a trend of generally rise.There were 1430 valid invention patents in total,among which 264 were invention patents and 1166 utility model patents.The total number of patens as well as invention patents were positive correlated to province GDP GDP and doctors per thousand population (r=0.824,0.812,0.784,0.771 respectively,P＜0.001).The rate of collaborative patent application was 6.28％,and there was a significant difference (α=0.05/3,P＜0.001) in the collaborative patent applications around Hangzhou,provincial and other cities medical and health institutions.Conclusions The total number of patents in 77 medical and health institutions in Zhejaing has reached a certain scale,and the number of patents increase with the economic development and the number of doctors per thousand population.The effective patents present in concentrating in regions,and the proportion of invention patents was low.

Objective To investigate the radioactivity distribution of 131 I-bovine serum albumin ( BSA )-mesoporous silica nanoparticles ( MSNs )-anti-vascular endothelial growth factor receptor 2 (VEGFR2) in anaplastic thyroid carcinoma (ATC) and to explore its antitumor efficacy in ATC-bearing nude mouse models. Methods 131 I-BSA-MSNs-anti-VEGFR2 and 131 I-BSA-MSNs were constructed. FRO tumor xenografts were established and the SPECT/CT images of tumor-bearing mice were acquired at differ-ent time points after intratumoral injection with 131 I-BSA-MSNs-anti-VEGFR2 ( targeting group) , 131 I-BSA-MSNs ( non-targeting group) , Na131 I ( Na131 I group) and saline ( control group) , respectively. The changes of body mass and tumor volume in each group were recorded. Two-sample t test and log-rank test were used to analyze the data. Results After incubation for 3 h, the fluorescence intensity in targeting group was higher than that in non-targeting group (345.26±16.35 vs 280.61±9.65;t=5.90, P<0.05). After injection for 1-3 weeks, the radioactivity detected by SPECT/CT in targeting group was obviously stronger than that in non-targeting group ( t values:7.060-12.780, all P<0.05) . At the end of the observation, the tumor vol-ume of Na131I group, control group, non-targeting group and targeting group increased to (278.3±19.3)％, (296.6±24.2)％, (198.7±13.2)％ and (103.7±6.2)％ of the original volume, respectively. The body mass of the first 2 groups decreased to (88.6±3.0)％ and (86.2±3.1)％ of the original body mass respec-tively, while that of the latter 2 groups increased to (102.1±3.1)％ and (116.2±3.4)％ of the original body mass respectively. Survival analysis showed that the median survival time in targeting group ( 38 d) was sig-nificantly longer than that in non-targeting group (34 d;χ2=8.05, P<0.05). Conclusion 131I-BSA-MSNs-anti-VEGFR2 can effectively inhibit the tumor growth of ATC and prolong the survival of tumor-bearing nude mice, which gives a good suggestion for the treatment and prognosis evaluation of ATC.