Background: Bacteria are among the common pathogens in Vietnam and other developing countries. Antibiotics are effective in the treatment, but the antibiotics gradually become ineffective due to drug resistance of bacteria, especially in children..\r\n', u'Objectives: Determine the root cause bacterial infections and often determine the extent of antibiotic resistance of common pathogenic bacteria isolated in Hai Phong pediatric Hospital.\r\n', u'Subjects and method: At Microorganism Department of Hai Phong pediatric Hospital from January 2003 to December 2004, 558 trains of common pathogenic microorganisms were isolated from 0 -15 year old children clinical samples such as: blood, pus, cerebrospinal fluid, urine, stool...These samples of isolated microorganisms were also tested on their sensitiveness to several antibiotics.\r\n', u'Results:Findings showed that the 3 most frequently recognized bacterier out of 11 antibiotic sensitive tested species were: streptococcus pneumoniae (20.61%), neisseria (18.1%), E.coli (15.95%); and the most resisted antibiotics included: Co-trimoxazole, Chloramphenicol and Ampiciline. The staph was resistant to general Chlora, phenicol, Co-trimoxazol, and also sensitive to cefotacim, Amikacin. In the bacillus bacteria tested were resistant blue latex all, only works with Amikacin.\r\n', u'Conclusion: Its also found that every kind of tested bacterium had got various levels of sensitivity to different of antibiotics.\r\n', u'
Anti-Bacterial Agents ; Bacteria/ microbiology

Background: The multiplex polymerase chain reaction can rapidly differentiate taenia spp adults and cysticercose cysts.\r\n', u'Objective: The study aimed to identify taenia spp adults and cysticercose cysts in human by using multiplex PCR\r\n', u'Subjects and methods: The multiplex PCR was applied by using the 4 forward and one reverse primer to amplify the target gene cytochrome c oxydase subunit I (COXI) of the Taenia spp in human. T\r\n', u'Results:4 molecular sizes of PCR products were appeared: 269 bp,720 bp, 827 bp, 984 bp. Seventy six samples including 65 flat worms and 11 cysticercose cysts which collected from the patients who are living in 19 different provinces and cities of Northern part of Viet Nam and treated in the clinic of NIMPE were examined. Of 65 flat worms analyzed 35 samples were T.asiatica (58.46%), 27 were T.saginata (41.54%). All 11 cysticercose cysts were T.solium (100%). \r\n', u'Conclusion: The result also indicated that some time 3 Taenia species were found at the same area. A remarkable difference of infection rate was found between men and women, also adults and children. \r\n', u'\r\n', u'
Taenia spp adults ; cysticercose cysts ; multiplex PCR

Background: Human albumin was produced and used in many countries. Cohn's technique had been used to precipitate albumin from human plasma. This technique was easy and cheap and the quality of the product was good. In Vietnam, human albumin had to import, but the prices was very expensive. Vietnam was having good plasma in large quantity and high quality. That\u2019s why research on production plasma albumin was essential.\r\n", u"Objectives: This study aimed at using Cohn's technique improved by Drohan and Van - Aken to produce standard albumin from human plasma. Subjects and method: Human plasma detected VIII-factor was used for present study. Plasma \ufffd?albumin was precipitated by ethanol at low temperature and pH. The collected albumins have been liophilizated and storage at 40C. The quality and quantity of Albumin was evaluated by quantitative analysis and protein \ufffd?electrophoresis. Results: The 418g of albumin powder was produced from 16 liters of plasma detected F \ufffd?VIII. The quality of this albumin come up to standard (>95%) and quantity of albumin collected from one liter of this plasma was 26g. Conclusion: In the Vietnamese condition, the technique of Cohn can be used to produce standard albumin for treatment.\r\n", u'
Albumins/ standards ; Plasma ;

Introduction: The need for gama \u2013 globulin, especially gama - globulin - anti \u2013 HBs, is huge in Vietnam. A number of patients cannot to afford use them due to the high price as they are imported. Meanwhile, Vietnam has high quality input sources for producing gama \u2013 globulin. \r\n', u'Objectives: To study the production process of gama - Globulin from Human Plasma and Standard gama - Globulin with Rich of Anti \u2013 HBs. \r\n', u'Subjects and method: 168 samples of human plasma from voluntary blood donors, which had been screened with for transfusion transmittable infections (TTLs), were chosen as plasma with rich of anti - HBs. The plasma with anti - HBsAg was precipitated with ethanol 25%, pH 6,9 to gain gama - globulin with rich anti - HBs, which was dried by Dutch Ly - 3 - TTE machine. Activation of anti - HBs gama - globulin was identified by a standard method of the degree of antibody specific for anti - HBsAg kit of BIORAD, \r\n', u'Results: The purity of the gama - globulin achieved was 93%, which was almost equal with the results of some foreign researchers (7.8), the activation of anti - HBs was 1:128 dilution degree. The productivity of gama - globulin gaining from 1 litter plasma was 6.0 gram. This new issue was first demonstrated in Vietnam. \r\n', u'Conclusion: We can domestically produce anti-Bs gama - globulin with high degree of activeness (1:128) from human plasma by the precipitating method with ethanol, pH and low temperature. \r\n', u'
Human plasma ; gama - globulin - anti - HBs ; Precipitation of ethanol ; pH and low temperature

An adolescent’s most common mental condition is depression. Adolescent depression is rising. This study examined characteristics that could affect adolescent depressive symptoms and if they would have a substantial impact on depressive disorders. 1,336 secondary and high school students from Thua Thien Hue province, Vietnam, were convenience sampled. This study examined how academic stress, self-rating life experiences, rejection, (over) protection, emotional warmth, social support, life satisfaction, and resilience can lead to depressive disorder. Academic stress, self-rating life events, rejection, and (over) protection were positively associated with depression symptoms among teenagers in Thua Thien Hue province, Vietnam. Depressive illnesses are also linked to life satisfaction, social support, and resilience. Academic stress dominated work contentment.

Background: Acute gastroenterophathy usually caused by the Rota virus for children under 5 years old. Objectives: To present various types of data on epidemiology of ROTA virus diarrhea in Ho Chi Minh city from 12/2006-11/2007. Material and method: The data were collected from 500 stool specimens of diarrhea diagnosed chilren hosptalised at Thuy Dien Pediatric hospital 1, Ho Chi Minh city from December/2006 to November /2007. Results:There were 322 rotavirus-positive specimens, representing 64.4%. The proportions of monthly distribution of cases with diarrhea due to rotavirus were 90.1%, 54.39%, 85.37%, 74.51%, 72.92%, 41.67%, 26.67%, 58.33%, 79.31%, 52.63%, 69.05% and 57.78%, respectively. The numbers of rotavirus-positive cases in male and female were 216 (65.26%) and 106 (62.72%), respectively. The proportions of Rota virus positive children compared to total number of diarrheal cases with age 0-3, 3-6, 6-12, 12-24, 24-36 and over 36 months were 2.80%, 7.76%, 40.06%, 40.68%, 5.28% and 3.42%, respectively.\r\n', u'The results of typing identification indicated that the phenotypes of 98 among 100 specimens were identified (98%) in which there were sixty-one specimens of G1P8 (61%), one specimen of G2P8 (1%), fourteen specimens of G3P8 (14%), four of specimens of G4P8 (4%), eighteen specimens of GmixedP8 (18%). There were only two specimens of GnontypeableP8 (2%). Conclusion: Further studies should be carried out to clear this issue.\r\n', u'
Rotavirus ; gel type.

Little is known about antibiotic-resistant Gram-negative bacteria (GNB) intestinal carriage among healthcare workers (HCWs) in Vietnam. All HCWs at a tertiary intensive care units were asked to undertake weekly rectal swabs. Among 40 participants, 65% (26/40) carried extended spectrum β-lactamases (ESBL)/AmpC β-lactamase-producing Escherichia coli. Two HCWs colonized with ESBL/AmpC β-lactamase-producing Klebsiella pneumoniae. One HCW colonized with Acinetobacter baumannii. No one carried Pseudomonas spp.. A quarter (10/40) of HCWs were identified as persistent and frequent carriers. There is an urgent need to screen antibiotic-resistant GNB among HCWs and improve HCWs' hand hygiene compliance to reduce the transmission of antibiotic-resistant GNB in the hospital.

Little is known about antibiotic-resistant Gram-negative bacteria (GNB) intestinal carriage among healthcare workers (HCWs) in Vietnam. All HCWs at a tertiary intensive care units were asked to undertake weekly rectal swabs. Among 40 participants, 65% (26/40) carried extended spectrum β-lactamases (ESBL)/AmpC β-lactamase-producing Escherichia coli. Two HCWs colonized with ESBL/AmpC β-lactamase-producing Klebsiella pneumoniae. One HCW colonized with Acinetobacter baumannii. No one carried Pseudomonas spp.. A quarter (10/40) of HCWs were identified as persistent and frequent carriers. There is an urgent need to screen antibiotic-resistant GNB among HCWs and improve HCWs' hand hygiene compliance to reduce the transmission of antibiotic-resistant GNB in the hospital.