1.Antibiotic resistance of common pathogenic bacteria isolated from Hai Phong pediatric Hospital in 2003 \ufffd?2004 year period
Sang Ngoc Nguyen ; Hanh Thi Doan ; Thanh Thi Hoang
Journal of Medical and Pharmaceutical Information 2001;6(6):22-27
Background: Bacteria are among the common pathogens in Vietnam and other developing countries. Antibiotics are effective in the treatment, but the antibiotics gradually become ineffective due to drug resistance of bacteria, especially in children..\r\n', u'Objectives: Determine the root cause bacterial infections and often determine the extent of antibiotic resistance of common pathogenic bacteria isolated in Hai Phong pediatric Hospital.\r\n', u'Subjects and method: At Microorganism Department of Hai Phong pediatric Hospital from January 2003 to December 2004, 558 trains of common pathogenic microorganisms were isolated from 0 -15 year old children clinical samples such as: blood, pus, cerebrospinal fluid, urine, stool...These samples of isolated microorganisms were also tested on their sensitiveness to several antibiotics.\r\n', u'Results:Findings showed that the 3 most frequently recognized bacterier out of 11 antibiotic sensitive tested species were: streptococcus pneumoniae (20.61%), neisseria (18.1%), E.coli (15.95%); and the most resisted antibiotics included: Co-trimoxazole, Chloramphenicol and Ampiciline. The staph was resistant to general Chlora, phenicol, Co-trimoxazol, and also sensitive to cefotacim, Amikacin. In the bacillus bacteria tested were resistant blue latex all, only works with Amikacin.\r\n', u'Conclusion: Its also found that every kind of tested bacterium had got various levels of sensitivity to different of antibiotics.\r\n', u'
Anti-Bacterial Agents
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Bacteria/ microbiology
2.Identification of taenia spp adults and cysticercose cysts in human by using multiplex PCR
Vien Viet Ha ; Dao Due Le ; Manh Due Nguyen ; Tan Van Hoang ; Nguyen Hanh Doan ; Nhung Thi Vu
Journal of Malaria and parasite diseases Control 2003;0(1):62-69
Background: The multiplex polymerase chain reaction can rapidly differentiate taenia spp adults and cysticercose cysts.\r\n', u'Objective: The study aimed to identify taenia spp adults and cysticercose cysts in human by using multiplex PCR\r\n', u'Subjects and methods: The multiplex PCR was applied by using the 4 forward and one reverse primer to amplify the target gene cytochrome c oxydase subunit I (COXI) of the Taenia spp in human. T\r\n', u'Results:4 molecular sizes of PCR products were appeared: 269 bp,720 bp, 827 bp, 984 bp. Seventy six samples including 65 flat worms and 11 cysticercose cysts which collected from the patients who are living in 19 different provinces and cities of Northern part of Viet Nam and treated in the clinic of NIMPE were examined. Of 65 flat worms analyzed 35 samples were T.asiatica (58.46%), 27 were T.saginata (41.54%). All 11 cysticercose cysts were T.solium (100%). \r\n', u'Conclusion: The result also indicated that some time 3 Taenia species were found at the same area. A remarkable difference of infection rate was found between men and women, also adults and children. \r\n', u'\r\n', u'
Taenia spp adults
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cysticercose cysts
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multiplex PCR