Aim In order to understand infective conditions of Clonorchis sinensis in different populations in Nantong.Methods The antibodies of Clonorchis sinensis in fishermen area and cooks and shopkeepers et al. were investigated with PVC-Fast-Dot-ELISA. Results The average detective rate of Clonorchis sinensis antibodies was 3.92％ (17/434),the detective rate of Clonorchis sinensis in residents of fishermen area, cooks and shopkeepers (6.45％ ,2/31,5.59％ ,9/161、4.29％ ,3/70)was obviously higher than that of in the normal control group(0. 86％ ,1/115). There was a significant difference in the statistics (X2 = 3.52,X2 = 4.01,X2 = 2.28,P＜0. 01 ). Conclusions ①There is human infection of Clonorchis sinensis in Nantong area. ②The data emphasizes that the infection of Clonorchis sinensis has a strong association to do with occupation and contacting with raw fishes and opportunity of eating raw fishes and shrimps. ③It is recommended that the people of common eating raw fishes and shrimps must be examined and treated regularly.