Humans ; Medicine, Chinese Traditional ; Psoriasis ; classification ; diagnosis ; therapy ; Syndrome

Objective To determine the value of soluble triggering receptor expressed on myeloid cells 1 (sTREM-1) in patients with stable chronic obstructive pulmonary disease (sCOPD). Methods From 2015 January to 2015 August, 101 patients with stable chronic obstructive pulmonary disease and 81 healthy controls were en-rolled. All subjects underwent pulmonary function test to record FEV1%pred and FEV1%FVC and their serum sTREM-1 levels were determined. Arterial blood gas analyses and COPD assessment tests were also conducted in stable COPD patients. Results Serum sTREM-1 levels were significantly higher in stable COPD patients than healthy controls (113.2 ± 31.5 pg/mL and 83.8 ± 17.9 pg/mL respectively, P=0.000). sTREM-1 levels were posi-tively correlated with CAT score (r=0.507, P=0.000), whereas negatively correlated with FEV1%pred and PaO2 (r = 0.507, P = 0.000; r = 0.439, P = 0.000). Conclusion sTREM-1 is a promising biomarker to evaluate sCOPD in the future.

To improve the quality of full English teaching of urology for foreign students,our experiences are: choose the excellent teaching staff,choose and prepare teaching material carefully,make CAI courseware accurately and popularly,enrich teaching component,pay attention to the background of the foreign students,and apply new teaching model with communications.

AIM:To observe the effect of rosiglitazone (RGZ) pretreatment on the expression of peroxisome proliferator-activated receptor γ( PPARγ) , nuclear factor E2-related factor 2 ( Nrf2 ) and heme oxygenase-1 ( HO-1 ) in the microglia cells activated by thrombin.METHODS:Microglia cells were obtained from the brain tissues of the newborn rats and were primarily cultured in vitro.After cultured for 14 d, the microglia cells were used in the experiment.The iso-lated microglia cells were randomly divided into normal control group, thrombin stimulation group ( TH group) , rosiglita-zone intervention group ( RGZ +TH group ) and retinoic acid intervention group ( RA +TH group ) .The expression of PPARγ, Nrf2 and HO-1 was observed by immunocytochemistry, real-time PCR and Western blot.RESULTS:The number of positive staining cells of PPARγ, Nrf2 and HO-1 in TH group, RGZ+TH group and RA+TH group were increased re-markably as compared with control group.The significant increases in PPARγ, Nrf2 and HO-1 were observed in RGZ+TH group compared with other groups.The mRNA expression of PPARγ, Nrf2 and HO-1 in RGZ+TH group was increased significantly as compared with TH group, control group or RA+TH group (P<0.01), Besides, the mRNA expression of Nrf2 and HO-1 in RA+TH group was decreased as compared with TH group or RGZ+TH group (P<0.01).The protein levels of PPARγ, Nrf2 and HO-1 in RGZ+TH group were significantly increased as compared with TH group, control group or RA+TH group (P<0.01).The protein expression of Nrf2 and HO-1 in RA+TH group was decreased as com-pared with TH group or RGZ+TH group (P<0.01).CONCLUSION:Rosiglitazone pretreatment might increase the ex-pression of PPARγ, Nrf2 and HO-1 in the microglia cells activated by thrombin.By inhibiting the expression of Nrf2 after RA pretreatment, the expression of the downstream gene HO-1 is also influenced.The anti-oxidative stress effects of rosigli-tazone might be achieved partly by modulating Nrf2 to control the downstream gene HO-1.

Objective To activate the microglia cells by using thrombin,and then to observe the effect of precondition of rosiglitazone (RGZ)-pretreated on the expression change of peroxisome proliferator-activated receptor-γ (PPARγ),nicotinamide adenine dinucleotide phosphate:quinone oxidoreductase (NQO1) and γ-glutamylcysteine synthetase (γ-GCS).Methods Microglia cells were obtained from the brain tissues of the newborn rats and were primary cultured in vitro.The microglia cells were isolated in 14 days.The isolated microglia cells were randomly devided into normal control group (control group),thrombin stimulation group (stimulation group) and rosiglitazone intervention group (RGZ + TH group).The PPARγ,NQO1 and γ-GCS were observed by immunocytochemistry and real-time polymerase chain reaction (RT-PCR) methods.Results The immunocytochemistry showed that the number of stained cells of PPARγ,NQO1 and γ-GCS in stimulation group and RGZ + TH group were increased remarkably as compared with the control group.A significant increase of the PPARγ,NQO1 and γ-GCS was observed in the RGZ + TH group compared to the others.The RT-PCR method demonstrated that the expressions of PPARγ mRNA(211.88 ± 58.75),NQO1 mRNA(182.67 ± 62.09) and γ-GCS mRNA (188.17 ± 57.06) in RGZ + TH group were increased significantly as compared with the stimulation group (119.19 ± 44.58,101.73±32.19,108.81 ±19.71) or the control group (0.34±0.21,0.73±0.46,0.30±0.13;F=181.50,286.63,614.43,all P ＜ 0.01).Conclusion Medium-dose rosiglitazone-pretreated might increase the expression of PPARγ,NQO1 and γ-GCS in microglia cells activated by thrombin.Rosiglitazone might activate the PPARγso that increase its downstream gene to achieve its anti-oxidative stress effects.

Acupuncture Points ; Acupuncture Therapy ; Chronic Disease ; therapy ; Edema ; therapy ; Heart Failure ; therapy ; Humans ; Male ; Middle Aged

Objective To observe the changes of macular choroidal thickness before and after laser treatment for diabetic retinopathy patients.Methods For patients with diabetes by fundus fluorescein angiography (FFA) examination,diagnosed as severe non-proliferative and proliferative diabetic retinopathy and in accordance with the laser photocoagulation treatment indications of 23 patients (45 eyes) included in the study.There were 10 cases of male,13 cases of female; the average age was (55.48±5.43) years old.All patients underwent macular grid laser photocoagulation and pan-retinal photocoagulation.The macular choroidal thickness before and after laser treatment was measured by enhanced depth imaging technique of optical coherence tomography during follow-up at 1,2,3 weeks and one month at specific sites of choroidal.The specific sites included subfoveal choroidal thickness (SFCT),from the foveal 1mm,3mm,6mm distance of nasal choroidal thickness (NCT) and temporal choroidal thickness (TCT).Results One week after the treatment,SFCT,NCT1 mm,NCT3 mm,TCT1 mm,TCT3 mm,TCT6 mm were obviously thickening (t=4.728,4.422,3.759,3.743,5.713,2.502; P＜0.05).Two weeks after the treatment the SFCT,NCT1 mm,NCT3 mm,TCT1 mm,TCT3 mm were decreased gradually (t =3.189,2.122,2.742,2.196,2.076 ; P＜0.05).The choroidal thickness returned to pretreatment level from 2 weeks to 4 weeks after treatment,the NCT6mm had no obvious change in the whole treatment period(P＞0.05).Conclusion The choroidal thickness was significantly thicker after laser photocoagulation treatment within 1 week,with the time prolonging the choroidal thickness gradually decreases.

OBJECTIVE:To observe the clinical efficacy of prostaglandin E1 used early in patients after renal transplantation. METHODS:93 renal transplant recipients (treatment group) were treated with PGE1(20 ?g?d-1) within 2 weeks after operation,and another 85 renal transplant recipients (control group) were not given PGE1 therapy in the same period. Urine volume,serum creatinine (Scr),blood flow resistance-indexes (RI) under Doppler color ultrasound,incidences of acute rejection (AR) and delayed graft function (DGF) and the 1 year patient/graft survival rates were compared between the two groups. RESULTS:The 24h urine volume in the treatment group on day 1 after operation was significantly higher than in the control group (9.40?1.9 L vs. 8.11?1.8L) (P

Objective: To explore the protections of GLP-Ⅱ pretreatment on mouse intestinal mucosal immunity after ischemia/reperfusion insult. Methods: Thirty ICR mice were randomly divided into three groups: namely normal control (N), control (C) and GLP-Ⅱ pretreatment (P). Group C and group P were inflicted with ligation of superior mesenteric artery for twenty minutes. The morphology of distal ileum mucosa, the rate of intestinal bacteria translocation, the level of plasma endotoxin and intestinal IgA were determined. Results: After ischemia/reperfusion insult, there were obvious damage at distal ileum mucosa in group C and obvious proliferation in group P.The rate of intestinal bacteria translocation and the level of plasma endotoxin were significantly increased (P