Objective To determine the change in expression of detrusor myosin heavy chain isoforms,SM1 and SM2,following bladder outlet obstruction (BOO) in rabbits. Methods Fourteen New Zealand white male rabbits were divided into 2 groups:control group (n=7) and obstruction group (n=7).The bladder necks were partially ligated to form BOO animal model in obstruction group.Then 5 weeks after operation the bladders were resected in both groups, and the weight and volume of the bladders were measured. After the detrusor tissue protein was extracted,SM1,SM2 and their mRNA were tested by western blotting and RT-PCR methods. Results The bladder weights of obstruction and control groups were (13.8?4.4) g and (3.7?0.5) g, respectively (P

Objective The purpose of this study was to evaluate computed tomography urography (CTU) and compared with conventional intravenous urography (IVU) in diagnosis of urological disease. Methods One hundred and sixty-five patients with 111 males and 54 females underwent CTU urography including 78 cases underwent IVU were reviewed in this study. There were 12 cases underwent retrograde pyelography. There were 75 cases of nephrolithiasis and ureterolithiasis, 30 cases of ureteropelvic junction obstruction (UPJO), 30 cases of bladder cancer, 15 cases of pyelogenic cyst and parapelvic cyst, 9 cases of megaloureter and 6 cases of cancer in pelvis and ureter. Results The accuracy of diagnosis was 94.5% (156/165) in CTU group and 46.7% (42/90) in IVU group.The accuracy of diagnosis was 100.0% (75/75)of nephrolithiasis and ureterolith in CTU group and 78. 6%(33/42)in IVU group, 90.0% (27/30) of UPJO in CTU group, 100.0% (30/30) of bladder cancer in CTU group and 75.0%(9/12)in IVU group, 80.0%(12/15)of pyelogenic cyst and parapelvic cyst in CTU group, 100.0% (9/9)of megaloureter in CTU group, 100.0% (6/6)of cancer in pelvis and ureter in CT group. The displayed rate of the distal end of stenosis and obstruction was 79.4%(81/102)in CTU group and 25.0%(15/60)in IVU group. The examination time was (18.9±8.4)min in CTU group and (67.1±26.7)min in IVU group. Conclusion The CTU can provide more information than conventional IVU and can replace the IVU as routine examination in most cases.

Objective To explore the diagnostic and treatment method of inflammatory bowel obstruction after surgery.Methods The clinical data of 35 patinets with inflammatory bowel obstruction after surgery were retrospectively analyzed.Results Through the conservative treatment,28 cases were cured,accounting for 80.0％,and 4cases were markedly effective,accounting for 11.4％,and 3 cases were effective,accounting for 8.6％,and total effective rate was 100.0％ ;The anal exhaust time was(3.1 ± 0.8)d,and the average defecation time was(3.3 ±0.8)d,and the bowel sounds return time was(2.4 ± 0.5 ) d,and the average completion time of disappearance of symptomswas ( 5.6 ± 0.8 ) d,and the time body temperature returned to normal was (3.0 ± 0.5 ) d,and the mean treatment time was (9.4 ± 1.2)d.Conclusion The treatment of postoperative intestinal obstruction should be used with caution in surgical treatment,and non-surgical conservative treatment was safe and feasible and effective.

Objective To compare the curative effect and safety between ziprasidone injection and haloperidol injection in the treatment of acute agitation of schizophrenia .Methods Totally 86 patients with acute agitation of schizophrenia were divided into observational group(n=43)and control group(n=43) ,the study used a random ,single‐blinded and clinical controlled experiment . the observational group was given ziprasidone mesylate 10-20 mg every time by intramuscular injection and the amount was less than 40 mg every day ;control group was given haloperidol injection 5-10 mg every time by intramuscular injection and the amount was less than 30 mg every day .Drugs in the two groups could be repeated according to the state of illness after 4-6 hours ,and the daily injections were no more than 3 times and the course of treatment was 3 days .The Positive and Negative Symptoms Scale Ex‐cited Factor(PANSS‐EC)was used to evaluate the agitated symptoms before treatment and 2 ,6 ,24 ,48 ,72 hours after treatment ,the Positive and Negative Symptoms Scale(PANSS)and Clinical Global Impression Scale‐Severity of Illness(CGI‐SI)was used to evalu‐ate the curative effect ;extrapyramidal side adverse reaction(SAS) ,Treatment Emergent Symptoms Scale(TESS)and the related lab tests were employed to assess the adverse reaction .Results Comparing with the baseline ,PANSS‐EC score of observe group de‐creased significantly at 2 h after the treatment ;at other observation time‐points ,PANSS scores ,PANSS‐EC scores and CGI‐SI scores in the two groups both decreased significantly(P<0 .01);but with no significant difference between the two groups(P>0 .05) .There were no significant difference in the total effective rate between the two groups(P>0 .05) .There were no serious ad‐verse events in the two groups .incidences of adverse reactions of observe group were 37 .21% ,which were significantly lower than that of control group 53 .49% (P<0 .05) .Conclusion Effect of ziprasidone injection and haloperidol injection was comparable in the treating acute agitation of schizophrenia ,which could treat acute agitation of schizophrenia with low incidences and excellent se‐curity .

Objective To investigate the anti-tumor effects of combined recombinant murine interleukin-12 and GM-CSF gene adenovirus vector(AdCMVIL-12,AdCMVGMCSF) on B16 melanoma.Methods B16 melanoma cells were injected subcutaneous at right back of C57/BL6 mice,48 tumor bearing mice were divided into 4 groups at random,when tumor diameter attained to 5 mm,AdCMVIL-12,AdCMVGM-CSF and AdCMVIL-12+AdCMVGM-CSF and AdCMVLacZ(control) were injected in different groups,tumor growth and immune responses of each group were tested,and the IL-12 and GMCSF levels in serum were observed.Results In combined group,necrosis, lymphocytes and macrophages were found in tumor tissues.30 d after treatment,the average volume in combined group was(60.73?11.36)mm~3, compared with AdCMVLacZ(control) group,and AdCMVIL-12 group,AdCMVGM-CSF group [(675.18?80.59),(186.91?19.15),(223.45?23.11)mm~3],there were significant differences(P

Objective To investigate the state of glucocorticoid(GC) - induced osteoporosis (GIOP) and the current prevention of GIOP in patients with primary glomerulonephritis. Methods Primary glomerulonephritis patients receiving GC therapy were observed and bone mineral density (BMD) in the lumbar spine and the femoral neck were measured. Age, sex, body - mass - index, smoking history, the time and accumulative dose of GC treatment, and the state of osteoporosis prevention were investigated, the factors that influence the BMD were analyzed. Results A total of one hundred and twenty- three patients were included in this study. Among them, osteoporosis and os-teopenia were found in 82 patients (66.7%). Lumbar spine BMD decrease gradually with the increase of the accumulative dose of GC. There were statistical differences in the BMD of lumbar spine in patients with receving GC at the period of less than 1 month compared with other groups( 1-12 months) (P

Objective To activate the microglia cells by using thrombin,and then to observe the effect of precondition of rosiglitazone (RGZ)-pretreated on the expression change of peroxisome proliferator-activated receptor-γ (PPARγ),nicotinamide adenine dinucleotide phosphate:quinone oxidoreductase (NQO1) and γ-glutamylcysteine synthetase (γ-GCS).Methods Microglia cells were obtained from the brain tissues of the newborn rats and were primary cultured in vitro.The microglia cells were isolated in 14 days.The isolated microglia cells were randomly devided into normal control group (control group),thrombin stimulation group (stimulation group) and rosiglitazone intervention group (RGZ + TH group).The PPARγ,NQO1 and γ-GCS were observed by immunocytochemistry and real-time polymerase chain reaction (RT-PCR) methods.Results The immunocytochemistry showed that the number of stained cells of PPARγ,NQO1 and γ-GCS in stimulation group and RGZ + TH group were increased remarkably as compared with the control group.A significant increase of the PPARγ,NQO1 and γ-GCS was observed in the RGZ + TH group compared to the others.The RT-PCR method demonstrated that the expressions of PPARγ mRNA(211.88 ± 58.75),NQO1 mRNA(182.67 ± 62.09) and γ-GCS mRNA (188.17 ± 57.06) in RGZ + TH group were increased significantly as compared with the stimulation group (119.19 ± 44.58,101.73±32.19,108.81 ±19.71) or the control group (0.34±0.21,0.73±0.46,0.30±0.13;F=181.50,286.63,614.43,all P ＜ 0.01).Conclusion Medium-dose rosiglitazone-pretreated might increase the expression of PPARγ,NQO1 and γ-GCS in microglia cells activated by thrombin.Rosiglitazone might activate the PPARγso that increase its downstream gene to achieve its anti-oxidative stress effects.

AIM:To observe the effect of rosiglitazone (RGZ) pretreatment on the expression of peroxisome proliferator-activated receptor γ( PPARγ) , nuclear factor E2-related factor 2 ( Nrf2 ) and heme oxygenase-1 ( HO-1 ) in the microglia cells activated by thrombin.METHODS:Microglia cells were obtained from the brain tissues of the newborn rats and were primarily cultured in vitro.After cultured for 14 d, the microglia cells were used in the experiment.The iso-lated microglia cells were randomly divided into normal control group, thrombin stimulation group ( TH group) , rosiglita-zone intervention group ( RGZ +TH group ) and retinoic acid intervention group ( RA +TH group ) .The expression of PPARγ, Nrf2 and HO-1 was observed by immunocytochemistry, real-time PCR and Western blot.RESULTS:The number of positive staining cells of PPARγ, Nrf2 and HO-1 in TH group, RGZ+TH group and RA+TH group were increased re-markably as compared with control group.The significant increases in PPARγ, Nrf2 and HO-1 were observed in RGZ+TH group compared with other groups.The mRNA expression of PPARγ, Nrf2 and HO-1 in RGZ+TH group was increased significantly as compared with TH group, control group or RA+TH group (P<0.01), Besides, the mRNA expression of Nrf2 and HO-1 in RA+TH group was decreased as compared with TH group or RGZ+TH group (P<0.01).The protein levels of PPARγ, Nrf2 and HO-1 in RGZ+TH group were significantly increased as compared with TH group, control group or RA+TH group (P<0.01).The protein expression of Nrf2 and HO-1 in RA+TH group was decreased as com-pared with TH group or RGZ+TH group (P<0.01).CONCLUSION:Rosiglitazone pretreatment might increase the ex-pression of PPARγ, Nrf2 and HO-1 in the microglia cells activated by thrombin.By inhibiting the expression of Nrf2 after RA pretreatment, the expression of the downstream gene HO-1 is also influenced.The anti-oxidative stress effects of rosigli-tazone might be achieved partly by modulating Nrf2 to control the downstream gene HO-1.

Female ; Gestational Age ; Globins ; Humans ; Infant, Newborn ; Infant, Premature ; Male ; Nerve Tissue Proteins ; blood

Objective To evaluate the long term efficacy of radical cystectomy and ileal conduit in the treatment of bladder cancer. Methods 196 cases of bladder tumor treated from Jan. 1985 to Jan. 2000 with radical cystectomy and ileal conduit for urinary diversion were studied retrospectively. Results Bladder neck or trigone was involved by the neogrowth in 126(64.3%).The growth has been invasive in 145(74.0%). 93.4%(183/196) of the tumor were transitional cell carcinoma. Early operative complication occurred in 19(9.7%) and late complication in 10(7.4%).On following up for 1 to 15 years with a mean of 6.6 years,the 5 year survival rate has been 66.2%. Conclusions Radical cystectomy is the optimal in treating invasive bladder tumor and ileal conduit is a good means of urinary diversion which is simple with minimal complications.