The glucose transporter(GLUT) is an energy-related carrier protein located on the cell membrane.Most researches have shown that changes of energy metabolism play an important role in the development of heart failure.This review summarizes recent advances in the understanding of the relationship between GLUT and heart failure.

Objective:To study the electrical safety of multi-parameter monitor and defibrillation monitor, and guarantee the safe and effective operation by through the medical equipment electrical safety performance in our hospital.Methods: To test 101 different models of multi-parameter monitor and defibrillation monitor electrical safety.Results: The one-time pass rate of 96%, and the two-time rate was 98%, also one multi-parameter monitor and defibrillation monitor hadn’t meet electrical safety performance after repairing.Conclusion: The equipments that don’t meet electrical safety performance prohibit using for clinical purposes; To ensure the safety of medical equipment and life safety of medical workers and patients by through developing the testing of the medical equipment electrical safety performance in our hospital.

Objective : To investigate the dynamic changes of the expression of GLUT1 mRNA and GLUT4 mRNA in rats myocardium with transient ischemia and reperfusion, and the relationship between the dynamic changes and the time during reperfusion. Methods : In rats, the left anterior descending coronary artery was occluded for 20 min followed by reperfusion for 4 hours, 1, 3 or 7 days as ischemia reperfusion model. The relative content of GLUT1 mRNA and GLUT4 mRNA in myocardium was detected by RT-PCR and gel electrophoresis imaging. Results: During myocardial post-ischemia and reperfusion, the levels of GLUT1 mRNA got up to the peak at 4th hour[ (0.666?0. 003 ) vs (0. 509?0.002) controls , P 0.05). Conclusion; Transient ischemia and reperfusion induce the expression of glucose transporters GLUT1 and GLUT4 genes in rat myocardi- um, which contribute to promote glucose utilization during ischemia, protect ischemic myocardium and improve functional recovery on reperfusion.

Objective:Preparations level by hospital accreditation and discussion hospital medical equipment quality and safety management indicators. Methods:Ministry of health, three general hospital accreditation standards implementing rules (2011 edition)(thejudging criteria) on hospital quality and safety of medical equipment management requirements research. Results:Establish five indicators hospital medical equipment quality and safety management, include adverse event monitoring indicators, strong inspection of measuring instruments management indicators, the use of personnel training management indicators, emergency life support equipment management class indicators, single-use sterile medical supplies and equipment management indicators. Conclusion:Proposed hospital quality and safety of medical equipment management indicators help to ensure the quality and safety of medical equipment, to provide a reference by exploring management indicators for the quality and safety management of medical equipment.

Objective: To study the effect of GLP-Ⅱ on residual bowel absorption of glucose in a rat model of short bowel syndrome. Methods: Rats undertaken 75% small bowel resection were randomly assigned to SB group and SB/GLP-Ⅱ group. Another group of weight-matched normal rats(Normal) received normal diet ad libitum was used as control group for SB group. All rats were allowed normal chow on the 1st postoperative day(POD). On the POD6,the intestinal glucose absorption data per unit length as well as per unit weight of ileum were measured by in vivo circulatory perfusion experiment. Results: Length of perfused ileum in 3 groups of rats were not significantly different. Wet weight to length ratio of perfused ileum in SB group was significantly higher than that of normal group,but significantly lower than that of SB/GLP-Ⅱ group. 45min glucose absorption of per cm ileum in SB/GLP-Ⅱ group was significantly higher than that of SB group(P0.05). Conclusions: In a rat model of SBS, GLP-Ⅱ can enhance the intestinal glucose absorption measured as per unit length of resudial ileum and GLP-Ⅱ manifests a trend to increase the glucose absorption measured as per unit wet weight of resudial ileum.

Objective To investigate the preventive effect of the artificial nerve canal on irritating neuralgia after neurilemmoma resection.Methods Thirty patients diagnosed as neurilemmoma were divided into artificial nerve group and control group at randomly.There were 15 patients in each group.The nerves in artificial nerve group were enclosed using the artificial nerve besides neurilemmoma resection.The nerves in control group only had neurilemmoma resection.All patients were assessed with VAS for the degree of irritating neuralgia at 3 time points after operation.Calculate the incidence of irritating neuralgia and analyze the data using statistical methods.Results In control group,the VAS scores were 7.5,5.1,2.8,and the incidences of irritating neuralgia were 80％,67％,40％,respectively.In artificial nerve group,the VAS scores were 3.2,1.5,0,and the incidences of irritating neuralgia were 33％,13％,0.The VAS scores and the incidences of irritating neuralgia of two groups had significant difference by statistical analysis (P ＜ 0.05).Conclusion The artificial nerve canal can relieve the degree and decrease the incidence of irritating neuralgia after neurilemmoma resection,which is a useful method to prevent irritating neuralgia in clinic.

Objective To investigate the effects of methamphetamine (Meth) on the outward K+ currents and elucidate the role of outward K+ channels in Meth induced hippocampal neuron damage.Methods Hippocampal neurons were harvest from 18-day-old embryonic rats and were divided into two groups:the control group and the Meth treated group.Both of 4-AP and TEA sensitive K+ currents were recorded after the treatment of Meth by performing the whole cell patch clamp.Furthermore,the MTT and TUNEL assays were performed to evaluate the effects of K+ channel on hippocampal neuron damage mediated by Meth.For statistical comparison,One-way ANOVA and LSD multiple comparison test or t-test was used.P-value ＜ 0.05 was considered to be statistically significant.Results The density of 4-AP sensitive K+ channel currents in Meth treated group [(120.1 ± 19.6) pA/pF,n =7] were significantly increased when compared with control group [(87.4 ± 12.5) pA/pF,n =10,P ＜0.01] and the increments of the currents induced by Meth was dose dependent.The MTT data showed that the cell viability was obviously decreased in Meth treated group (48.72 ± 4.38) ％ relative to the control group (100.07 ± 3.36) ％.Moreover,application of K+ channel antagonist,4-AP (61.39 ± 3.15)％,and the high K+ solution (78.25 ± 9.42) ％ substantially enhanced the cell viability.The TUNEL assay showed there were protective effects of 4-AP and the high K+ solution against neuron damage observed during cells exposed to Meth.Conclusions The increments of 4-AP sensitive K+ channel currents induced by Meth might be involved in hippocampal neuron damage.

Objective To investigate the role of Wnt/β-catenin pathway in the glucocorticoids (GC)-mediated Alzheimer' s disease-like pathological changes in vitro.Methods Human embryonic kidney 293 (HEK293/wt) cells stably transfected with the longest human tau (tau441,HEK293/tau) and wild-type HEK293 cells were employed to study the role of GC.Cell viabilities of the two cell lines were examined by cell counting kit-8 ( CCK-8 ).Levels of phosphorylated tau ( p-T205 ) and dephosphorylated tau (Tau-1),β-catenin,phosphorylated β-catenin (p-β-catenin),glycogen synthase kinase-3β (GSK-3β),phosphorylated GSK-3β at Ser9 (ps9-GSK-3β) and Bcl-2 were determined by Western blotting.Results Treatment with 1 μmol/L GC for 48 h decreased the viability of HEK293/wt and HEK293/tau cells to 95.5％ ±3.2％ and 77.8％ ± 4.4％ (t =6.60,P ＜ 0.05 ).Moreover,GC treatment decreased the levels of ps9-GSK-3β,Tau-1,β-catenin and Bcl-2 to 47.8％ ± 10.4％,53.9％ ± 11.7％,50.9％ ±7.6％,48.4％ ±6.5％ of control groups ( t =7.01,3.86,7.09,7.30,all P ＜ 0.05 ),and increased the relative levels of pT205,p-β-catenin to 180.5％ ± 22.2％,201.3 ％ ± 27.6％ of control groups (t =5.51,5.27,both P ＜0.05) only in HEK293/tau cells.Finally,LiCI efficiently prevented the above effects of GC in HEK293/tau cells.Conclusion GC may trigger Alzheimer' s disease-like pathological changes by inhibiting the Wnt/β-catenin pathway and these pathological processes seem to specifically depend on the presence of human tau.

Objective To evaluate the treatment methods of endophthalmitis after IOL implantation. Design Retrospective case series. Participants 15 patients with endophthalmitis after IOL implantation treated by Beijing Xuanwu Hospital in 2002-2008. Meth-ods Vancomycin was injected into vitreous cavity in all patients. Vitrectomy was performed on the patient whose infective bacteria could not be controlled by intravitreal injection. Main outcome measures Infective status, visual function, results of microbiological exami-nation. Result Endophthalmitis occured in 13 cases (86.6%) after 72 hours of cartaract surgery. Microbiological examination was per-formed on 15 cases (aqueous or vitreous sample), 6 cases showed positive results, in which 4 cases were staphylococcus epidermidis. The infection of 12 patients (80%) were controlled with intravitreal injection and 10 patients gained final visual acuity better than 0.1. Three patients received vitrectomy because infective bacteria can not be controlled. Conclusion Staphylococcus epi. may be the major cause of subacute endophthalmitis after cartaract surgery. Antibiotics injected immediately into vitreous cavity can control the infections in most cases.(Ophthalmol CHN, 2009, 18: 270-272)

Adolescent ; Adult ; Cytarabine ; administration & dosage ; blood ; pharmacology ; Drug Interactions ; Female ; Humans ; Instillation, Drug ; Leukemia ; blood ; drug therapy ; Male ; Methotrexate ; administration & dosage ; blood ; pharmacology ; Middle Aged ; Young Adult